名称: | pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE |
---|---|
别称: | Plasmid#60910 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 蓝色 |
启动子: | dSV40 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pHR |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGCA
TCTAGAATTA
ATTCCGTGTA
TTCTATAGTG
TCACCTAAAT
CGTATGTGTA
TGATACATAA
GGTTATGTAT
TAATTGTAGC
CGCGTTCTAA
CGACAATATG
TACAAGCCTA
ATTGTGTAGC
ATCTGGCTTA
CTGAAGCAGA
CCCTATCATC
TCTCTCGTAA
ACTGCCGTCA
GAGTCGGTTT
GGTTGGACGA
ACCTTCTGAG
TTTCTGGTAA
CGCCGTCCCG
CACCCGGAAA
TGGTCAGCGA
ACCAATCAGC
AGGGTCATCG
CTAGCCAGAT
CCTCTACGCC
GGACGCATCG
TGGCCGGCAT
CACCGGCGCC
ACAGGTGCGG
TTGCTGGCGC
CTATATCGCC
GACATCACCG
ATGGGGAAGA
TCGGGCTCGC
CACTTCGGGC
TCATGAGCGC
TTGTTTCGGC
GTGGGTATGG
TGGCAGGCCC
CGTGGCCGGG
GGACTGTTGG
GCGCCATCTC
CTTGCATGCA
CCATTCCTTG
CGGCGGCGGT
GCTCAACGGC
CTCAACCTAC
TACTGGGCTG
CTTCCTAATG
CAGGAGTCGC
ATAAGGGAGA
GCGTCGAATG
GTGCACTCTC
AGTACAATCT
AGCTCTGATG
CCGCATAGTT
AAGCCAGCCC
CGACACCCGC
CAACACCCGC
TGACGCGCCC
TGACGGGCTT
GTCTGCTCCC
GGCATCCGCT
TACAGACAAG
CTGTGACCGT
CTCCGGGAGC
TGCATGTGTC
AGAGGTTTTC
ACCGTCATCA
CCGAAACGCG
CGAGACGAAA
GGGCCTCGTG
ATACGCCTAT
TTTTATAGGT
TAATGTCATG
ATAATAATGG
TTTCTTAGAC
GTCAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGG
TCTCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTATTACCGC
CTTTGAGTGA
GCTGATACCG
CTCGCCGCAG
CCGAACGACC
GAGCGCAGCG
AGTCAGTGAG
CGAGGAAGCG
GAAGAGCGCC
CAATACGCAA
ACCGCCTCTC
CCCGCGCGTT
GGCCGATTCA
TTAATGCAGC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAAA
GCTTGGACAC
AAGACAGGCT
TGCGAGATAT
GTTTGAGAAT
ACCACTTTAT
CCCGCGTCAG
GGAGAGGCAG
TGCGTAAAAA
GACGCGGACT
CATGTGAAAT
ACTGGTTTTT
AGTGCGCCAG
ATCTCTATAA
TCTCGCGCAA
CCTATTTTCC
CCTCGAACAC
TTTTTAAGCC
GTAGATAAAC
AGGCTGGGAC
ACTTCACATG
AGCGAAAAAT
ACATCGTCAC
CTGGGACATG
TTGCAGATCC
ATGCACGTAA
ACTCGCAAGC
CGACTGATGC
CTTCTGAACA
ATGGAAAGGC
ATTATTGCCG
TAAGCCGTGG
CGGTCTGTAC
CGGGTGCGTT
ACTGGCGCGT
GAACTGGGTA
TTCGTCATGT
CGATACCGTT
TGTATTTCCA
GCTACGATCA
CGACAACCAG
CGCGAGCTTA
AAGTGCTGAA
ACGCGCAGAA
GGCGATGGCG
AAGGCTTCAT
CGTTATTGAT
GACCTGGTGG
ATACCGGTGG
TACTGCGGTT
GCGATTCGTG
AAATGTATCC
AAAAGCGCAC
TTTGTCACCA
TCTTCGCAAA
ACCGGCTGGT
CGTCCGCTGG
TTGATGACTA
TGTTGTTGAT
ATCCCGCAAG
ATACCTGGAT
TGAACAGCCG
TGGGATATGG
GCGTCGTATT
CGTCCCGCCA
ATCTCCGGTC
GCTAATCTTT
TCAACGCCTG
GCACTGCCGG
GCGTTGTTCT
TTTTAACTTC
AGGCGGGTTA
CAATAGTTTC
CAGTAAGTAT
TCTGGAGGCT
GCATCCATGA
CACAGGCAAA
CCTGAGCGAA
ACCCTGTTCA
AACCCCGCTT
TAAACATCCT
GAAACCTCGA
CGCTAGTCCG
CCGCTTTAAT
CACGGCGCAC
AACCGCCTGT
GCAGTCGGCC
CTTGATGGTA
AAACCATCCC
TCACTGGTAT
CGCATGATTA
ACCGTCTGAT
GTGGATCTGG
CGCGGCATTG
ACCCACGCGA
AATCCTCGAC
GTCCAGGCAC
GTATTGTGAT
GAGCGATGCC
GAACGTACCG
ACGATGATTT
ATACGATACG
GTGATTGGCT
ACCGTGGCGG
CAACTGGATT
TATGAGTGGG
CCCCGGATCT
TTGTGAAGGA
ACCTTACTTC
TGTGGTGTGA
CATAATTGGA
CAAACTACCT
ACAGAGATTT
AAAGCTCTAA
GGTAAATATA
AAATTTTTAA
GTGTATAATG
TGTTAAACTA
CTGATTCTAA
TTGTTTGTGT
ATTTTAGATT
CCAACCTATG
GAACTGATGA
ATGGGAGCAG
TGGTGGAATG
CCTTTAATGA
GGAAAACCTG
TTTTGCTCAG
AAGAAATGCC
ATCTAGTGAT
GATGAGGCTA
CTGCTGACTC
TCAACATTCT
ACTCCTCCAA
AAAAGAAGAG
AAAGGTAGAA
GACCCCAAGG
ACTTTCCTTC
AGAATTGCTA
AGTTTTTTGA
GTCATGCTGT
GTTTAGTAAT
AGAACTCTTG
CTTGCTTTGC
TATTTACACC
ACAAAGGAAA
AAGCTGCACT
GCTATACAAG
AAAATTATGG
AAAAATATTC
TGTAACCTTT
ATAAGTAGGC
ATAACAGTTA
TAATCATAAC
ATACTGTTTT
TTCTTACTCC
ACACAGGCAT
AGAGTGTCTG
CTATTAATAA
CTATGCTCAA
AAATTGTGTA
CCTTTAGCTT
TTTAATTTGT
AAAGGGGTTA
ATAAGGAATA
TTTGATGTAT
AGTGCCTTGA
CTAGAGATCA
TAATCAGCCA
TACCACATTT
GTAGAGGTTT
TACTTGCTTT
AAAAAACCTC
CCACACCTCC
CCCTGAACCT
GAAACATAAA
ATGAATGCAA
TTGTTGTTGT
TAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGGATCAACT
GGATAACTCA
AGCTAACCAA
AATCATCCCA
AACTTCCCAC
CCCATACCCT
ATTACCACTG
CCAATTACCT
AGTGGTTTCA
TTTACTCTAA
ACCTGTGATT
CCTCTGAATT
ATTTTCATTT
TAAAGAAATT
GTATTTGTTA
AATATGTACT
ACAAACTTAG
TAGTTGGAAG
GGCTAATTCA
CTCCCAAAGA
AGACAAGATA
TCCTTGATCT
GTGGATCTAC
CACACACAAG
GCTACTTCCC
TGATTAGCAG
AACTACACAC
CAGGGCCAGG
GGTCAGATAT
CCACTGACCT
TTGGATGGTG
CTACAAGCTA
GTACCAGTTG
AGCCAGATAA
GGTAGAAGAG
GCCAATAAAG
GAGAGAACAC
CAGCTTGTTA
CACCCTGTGA
GCCTGCATGG
GATGGATGAC
CCGGAGAGAG
AAGTGTTAGA
GTGGAGGTTT
GACAGCCGCC
TAGCATTTCA
TCACGTGGCC
CGAGAGCTGC
ATCCGGAGTA
CTTCAAGAAC
TGCTGATATC
GAGCTTGCTA
CAAGGGACTT
TCCGCTGGGG
ACTTTCCAGG
GAGGCGTGGC
CTGGGCGGGA
CTGGGGAGTG
GCGAGCCCTC
AGATCCTGCA
TATAAGCAGC
TGCTTTTTGC
CTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
GGCGCCCGAA
CAGGGACTTG
AAAGCGAAAG
GGAAACCAGA
GGAGCTCTCT
CGACGCAGGA
CTCGGCTTGC
TGAAGCGCGC
ACGGCAAGAG
GCGAGGGGCG
GCGACTGGTG
AGTACGCCAA
AAATTTTGAC
TAGCGGAGGC
TAGAAGGAGA
GAGATGGGTG
CGAGAGCGTC
AGTATTAAGC
GGGGGAGAAT
TAGATCGCGA
TGGGAAAAAA
TTCGGTTAAG
GCCAGGGGGA
AAGAAAAAAT
ATAAATTAAA
ACATATAGTA
TGGGCAAGCA
GGGAGCTAGA
ACGATTCGCA
GTTAATCCTG
GCCTGTTAGA
AACATCAGAA
GGCTGTAGAC
AAATACTGGG
ACAGCTACAA
CCATCCCTTC
AGACAGGATC
AGAAGAACTT
AGATCATTAT
ATAATACAGT
AGCAACCCTC
TATTGTGTGC
ATCAAAGGAT
AGAGATAAAA
GACACCAAGG
AAGCTTTAGA
CAAGATAGAG
GAAGAGCAAA
ACAAAAGTAA
GACCACCGCA
CAGCAAGCGG
CCGGTGATCT
TCAGACCTGG
ACGATATATA
TGAGGGACAA
TTGGAGAAGT
GAATTATATA
AATATAAAGT
AGTAAAAATT
GAACCATTAG
GAGTAGCACC
CACCAAGGCA
AAGAGAAGAG
TGGTGCAGAG
AGAAAAAAGA
GCAGTGGGAA
TAGGAGCTTT
GTTCCTTGGG
TTCTTGGGAG
CAGCAGGAAG
CACTATGGGC
GCAGCGTCAA
TGACGCTGAC
GGTACAGGCC
AGACAATTAT
TGTCTGGTAT
AGTGCAGCAG
CAGAACAATT
TGCTGAGGGC
TATTGAGGCG
CAACAGCATC
TGTTGCAACT
CACAGTCTGG
GGCATCAAGC
AGCTCCAGGC
AAGAATCCTG
GCTGTGGAAA
GATACCTAAA
GGATCAACAG
CTCCTGGGGA
TTTGGGGTTG
CTCTGGAAAA
CTCATTTGCA
CCACTGCTGT
GCCTTGGAAT
GCTAGTTGGA
GTAATAAATC
TCTGGAACAG
ATTTGGAATC
ACACGACCTG
GATGGAGTGG
GACAGAGAAA
TTAACAATTA
CACAAGCTTA
ATACACTCCT
TAATTGAAGA
ATCGCAAAAC
CAGCAAGAAA
AGAATGAACA
AGAATTATTG
GAATTAGATA
AATGGGCAAG
TTTGTGGAAT
TGGTTTAACA
TAACAAATTG
GCTGTGGTAT
ATAAAATTAT
TCATAATGAT
AGTAGGAGGC
TTGGTAGGTT
TAAGAATAGT
TTTTGCTGTA
CTTTCTATAG
TGAATAGAGT
TAGGCAGGGA
TATTCACCAT
TATCGTTTCA
GACCCACCTC
CCAACCCCGA
GGGGACCCGA
CAGGCCCGAA
GGAATAGAAG
AAGAAGGTGG
AGAGAGAGAC
AGAGACAGAT
CCATTCGATT
AGTGAACGGA
TCTCGACGGT
ATCGCCAAAT
GGCAGTATTC
ATCCACAATT
TTAAAAGAAA
AGGGGGGATT
GGGGGGTACA
GTGCAGGGGA
AAGAATAGTA
GACATAATAG
CAACAGACAT
ACAAACTAAA
GAATTACAAA
AACAAATTAC
AAAAATTCAA
AATTTTCGGG
TTTATTACAG
GGACAGCAGA
GATCCAGTTT
GGATCGATAA
GCTTGATATC
GAATTCAGGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCATAGTCC
CGCCCCTAAC
TCCGCCCATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCTGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGGCTA
CGCGCGCCAC
CATGCCCAAG
AAGAAGCGCA
AGGTGGGACG
CGTCTGCAGG
ATATCAAGCT
TGCGGTACCG
CGGGCCCGGG
ATCGCCACCA
TGGACAAGAA
GTACAGCATC
GGCCTGGCCA
TCGGCACCAA
CTCTGTGGGC
TGGGCCGTGA
TCACCGACGA
GTACAAGGTG
CCCAGCAAGA
AATTCAAGGT
GCTGGGCAAC
ACCGACCGGC
ACAGCATCAA
GAAGAACCTG
ATCGGCGCCC
TGCTGTTCGA
CAGCGGAGAA
ACAGCCGAGG
CCACCCGGCT
GAAGAGAACC
GCCAGAAGAA
GATACACCAG
ACGGAAGAAC
CGGATCTGCT
ATCTGCAAGA
GATCTTCAGC
AACGAGATGG
CCAAGGTGGA
CGACAGCTTC
TTCCACAGAC
TGGAAGAGTC
CTTCCTGGTG
GAAGAGGATA
AGAAGCACGA
GCGGCACCCC
ATCTTCGGCA
ACATCGTGGA
CGAGGTGGCC
TACCACGAGA
AGTACCCCAC
CATCTACCAC
CTGAGAAAGA
AACTGGTGGA
CAGCACCGAC
AAGGCCGACC
TGCGGCTGAT
CTATCTGGCC
CTGGCCCACA
TGATCAAGTT
CCGGGGCCAC
TTCCTGATCG
AGGGCGACCT
GAACCCCGAC
AACAGCGACG
TGGACAAGCT
GTTCATCCAG
CTGGTGCAGA
CCTACAACCA
GCTGTTCGAG
GAAAACCCCA
TCAACGCCAG
CGGCGTGGAC
GCCAAGGCCA
TCCTGTCTGC
CAGACTGAGC
AAGAGCAGAC
GGCTGGAAAA
TCTGATCGCC
CAGCTGCCCG
GCGAGAAGAA
GAATGGCCTG
TTCGGCAACC
TGATTGCCCT
GAGCCTGGGC
CTGACCCCCA
ACTTCAAGAG
CAACTTCGAC
CTGGCCGAGG
ATGCCAAACT
GCAGCTGAGC
AAGGACACCT
ACGACGACGA
CCTGGACAAC
CTGCTGGCCC
AGATCGGCGA
CCAGTACGCC
GACCTGTTTC
TGGCCGCCAA
GAACCTGTCC
GACGCCATCC
TGCTGAGCGA
CATCCTGAGA
GTGAACACCG
AGATCACCAA
GGCCCCCCTG
AGCGCCTCTA
TGATCAAGAG
ATACGACGAG
CACCACCAGG
ACCTGACCCT
GCTGAAAGCT
CTCGTGCGGC
AGCAGCTGCC
TGAGAAGTAC
AAAGAGATTT
TCTTCGACCA
GAGCAAGAAC
GGCTACGCCG
GCTACATCGA
TGGCGGAGCC
AGCCAGGAAG
AGTTCTACAA
GTTCATCAAG
CCCATCCTGG
AAAAGATGGA
CGGCACCGAG
GAACTGCTCG
TGAAGCTGAA
CAGAGAGGAC
CTGCTGCGGA
AGCAGCGGAC
CTTCGACAAC
GGCAGCATCC
CCCACCAGAT
CCACCTGGGA
GAGCTGCACG
CCATTCTGCG
GCGGCAGGAA
GATTTTTACC
CATTCCTGAA
GGACAACCGG
GAAAAGATCG
AGAAGATCCT
GACCTTCCGC
ATCCCCTACT
ACGTGGGCCC
TCTGGCCAGG
GGAAACAGCA
GATTCGCCTG
GATGACCAGA
AAGAGCGAGG
AAACCATCAC
CCCCTGGAAC
TTCGAGGAAG
TGGTGGACAA
GGGCGCCAGC
GCCCAGAGCT
TCATCGAGCG
GATGACCAAC
TTCGATAAGA
ACCTGCCCAA
CGAGAAGGTG
CTGCCCAAGC
ACAGCCTGCT
GTACGAGTAC
TTCACCGTGT
ACAACGAGCT
GACCAAAGTG
AAATACGTGA
CCGAGGGAAT
GAGAAAGCCC
GCCTTCCTGA
GCGGCGAGCA
GAAAAAAGCC
ATCGTGGACC
TGCTGTTCAA
GACCAACCGG
AAAGTGACCG
TGAAGCAGCT
GAAAGAGGAC
TACTTCAAGA
AAATCGAGTG
CTTCGACTCC
GTGGAAATCT
CCGGCGTGGA
AGATCGGTTC
AACGCCTCCC
TGGGCACATA
CCACGATCTG
CTGAAAATTA
TCAAGGACAA
GGACTTCCTG
GACAATGAGG
AAAACGAGGA
CATTCTGGAA
GATATCGTGC
TGACCCTGAC
ACTGTTTGAG
GACAGAGAGA
TGATCGAGGA
ACGGCTGAAA
ACCTATGCCC
ACCTGTTCGA
CGACAAAGTG
ATGAAGCAGC
TGAAGCGGCG
GAGATACACC
GGCTGGGGCA
GGCTGAGCCG
GAAGCTGATC
AACGGCATCC
GGGACAAGCA
GTCCGGCAAG
ACAATCCTGG
ATTTCCTGAA
GTCCGACGGC
TTCGCCAACA
GAAACTTCAT
GCAGCTGATC
CACGACGACA
GCCTGACCTT
TAAAGAGGAC
ATCCAGAAAG
CCCAGGTGTC
CGGCCAGGGC
GATAGCCTGC
ACGAGCACAT
TGCCAATCTG
GCCGGCAGCC
CCGCCATTAA
GAAGGGCATC
CTGCAGACAG
TGAAGGTGGT
GGACGAGCTC
GTGAAAGTGA
TGGGCCGGCA
CAAGCCCGAG
AACATCGTGA
TCGAAATGGC
CAGAGAGAAC
CAGACCACCC
AGAAGGGACA
GAAGAACAGC
CGCGAGAGAA
TGAAGCGGAT
CGAAGAGGGC
ATCAAAGAGC
TGGGCAGCCA
GATCCTGAAA
GAACACCCCG
TGGAAAACAC
CCAGCTGCAG
AACGAGAAGC
TGTACCTGTA
CTACCTGCAG
AATGGGCGGG
ATATGTACGT
GGACCAGGAA
CTGGACATCA
ACCGGCTGTC
CGACTACGAT
GTGGACGCTA
TCGTGCCTCA
GAGCTTTCTG
AAGGACGACT
CCATCGATAA
CAAAGTGCTG
ACTCGGAGCG
ACAAGAACCG
GGGCAAGAGC
GACAACGTGC
CCTCCGAAGA
GGTCGTGAAG
AAGATGAAGA
ACTACTGGCG
CCAGCTGCTG
AATGCCAAGC
TGATTACCCA
GAGGAAGTTC
GACAATCTGA
CCAAGGCCGA
GAGAGGCGGC
CTGAGCGAAC
TGGATAAGGC
CGGCTTCATC
AAGAGACAGC
TGGTGGAAAC
CCGGCAGATC
ACAAAGCACG
TGGCACAGAT
CCTGGACTCC
CGGATGAACA
CTAAGTACGA
CGAGAACGAC
AAACTGATCC
GGGAAGTGAA
AGTGATCACC
CTGAAGTCCA
AGCTGGTGTC
CGATTTCCGG
AAGGATTTCC
AGTTTTACAA
AGTGCGCGAG
ATCAACAACT
ACCACCACGC
CCACGACGCC
TACCTGAACG
CCGTCGTGGG
AACCGCCCTG
ATCAAAAAGT
ACCCTAAGCT
GGAAAGCGAG
TTCGTGTACG
GCGACTACAA
GGTGTACGAC
GTGCGGAAGA
TGATCGCCAA
GAGCGAGCAG
GAAATCGGCA
AGGCTACCGC
CAAGTACTTC
TTCTACAGCA
ACATCATGAA
CTTTTTCAAG
ACCGAGATTA
CCCTGGCCAA
CGGCGAGATC
CGGAAGCGGC
CTCTGATCGA
GACAAACGGC
GAAACAGGCG
AGATCGTGTG
GGATAAGGGC
CGGGACTTTG
CCACCGTGCG
GAAAGTGCTG
TCTATGCCCC
AAGTGAATAT
CGTGAAAAAG
ACCGAGGTGC
AGACAGGCGG
CTTCAGCAAA
GAGTCTATCC
TGCCCAAGAG
GAACAGCGAC
AAGCTGATCG
CCAGAAAGAA
GGACTGGGAC
CCTAAGAAGT
ACGGCGGCTT
CGACAGCCCC
ACCGTGGCCT
ATTCTGTGCT
GGTGGTGGCC
AAAGTGGAAA
AGGGCAAGTC
CAAGAAACTG
AAGAGTGTGA
AAGAGCTGCT
GGGGATCACC
ATCATGGAAA
GAAGCAGCTT
CGAGAAGAAT
CCCATCGACT
TTCTGGAAGC
CAAGGGCTAC
AAAGAAGTGA
AAAAGGACCT
GATCATCAAG
CTGCCTAAGT
ACTCCCTGTT
CGAGCTGGAA
AACGGCCGGA
AGAGAATGCT
GGCCTCTGCC
GGCGAACTGC
AGAAGGGAAA
CGAACTGGCC
CTGCCCTCCA
AATATGTGAA
CTTCCTGTAC
CTGGCCAGCC
ACTATGAGAA
GCTGAAGGGC
TCCCCCGAGG
ATAATGAGCA
GAAACAGCTG
TTTGTGGAAC
AGCACAAACA
CTACCTGGAC
GAGATCATCG
AGCAGATCAG
CGAGTTCTCC
AAGAGAGTGA
TCCTGGCCGA
CGCTAATCTG
GACAAGGTGC
TGAGCGCCTA
CAACAAGCAC
AGAGACAAGC
CTATCAGAGA
GCAGGCCGAG
AATATCATCC
ACCTGTTTAC
CCTGACCAAT
CTGGGAGCCC
CTGCCGCCTT
CAAGTACTTT
GACACCACCA
TCGACCGGAA
GAGGTACACC
AGCACCAAAG
AGGTGCTGGA
CGCCACCCTG
ATCCACCAGA
GCATCACCGG
CCTGTACGAG
ACACGGATCG
ACCTGTCTCA
GCTGGGAGGC
GACGCCTATC
CCTATGACGT
GCCCGATTAT
GCCAGCCTGG
GCAGCGGCTC
CCCCAAGAAA
AAACGCAAGG
TGGAAGATCC
TAAGAAAAAG
CGGAAAGTGG
ACGGCATTGG
TAGTGGGAGC
AACGGCAGCA
GCGGATCCAA
CGGTCCGGGT
GGATCTGGAG
GTGGAGGTTC
TGGAGGAGAA
GAACTTTTGA
GCAAGAATTA
TCATCTTGAG
AACGAAGTGG
CTCGTCTTAA
GAAAGGTTCT
GGCAGTGGAG
AAGAACTGCT
TTCAAAGAAT
TACCACCTGG
AAAATGAGGT
AGCTAGACTG
AAAAAGGGGA
GCGGAAGTGG
GGAGGAGTTG
CTGAGCAAAA
ATTATCATTT
GGAGAACGAA
GTAGCACGAC
TAAAGAAAGG
GTCCGGATCG
GGTGAGGAGT
TACTCTCGAA
AAATTATCAT
CTCGAAAACG
AAGTGGCTCG
GCTAAAAAAG
GGCAGTGGTT
CTGGAGAAGA
GCTATTATCT
AAAAACTACC
ACCTCGAAAA
TGAGGTGGCA
CGCTTAAAAA
AGGGAAGTGG
CAGTGGTGAA
GAGCTACTAT
CCAAGAATTA
TCATCTTGAG
AACGAGGTAG
CGCGTTTGAA
GAAGGGTTCC
GGCTCAGGAG
AGGAACTGCT
CTCGAAGAAC
TATCATCTTG
AAAATGAGGT
CGCTCGATTA
AAAAAGGGAT
CGGGCAGTGG
TGAGGAACTA
CTTTCAAAGA
ATTACCACCT
CGAAAACGAA
GTAGCTCGAT
TAAAGAAAGG
TTCAGGGTCG
GGTGAAGAAT
TACTGAGTAA
AAATTATCAT
CTGGAAAATG
AGGTAGCGAG
ACTAAAAAAG
GGGAGTGGTT
CTGGCGAAGA
GTTGCTATCG
AAAAATTATC
ATCTTGAGAA
CGAAGTTGCT
AGGCTCAAAA
AGGGCTCAGG
CTCAGGCGAG
GAGTTGCTCT
CGAAAAACTA
CCACTTGGAA
AATGAGGTCG
CGAGGTTGAA
AAAGGGGAGC
GGGTCGGGCG
AGGAGTTATT
GAGCAAAAAC
TATCATTTAG
AGAACGAAGT
CGCGCGCTTA
AAGAAAGGCT
CGGGCTCGGG
CGAAGAACTC
TTATCGAAGA
ACTACCACCT
CGAAAATGAG
GTCGCCAGGT
TGAAAAAGGG
CAGTGGCAGC
GGGGAGGAAC
TCTTGAGCAA
GAACTACCAC
TTGGAGAATG
AGGTCGCGAG
ATTGAAGAAA
GGGTCGGGGA
GCGGCGAGGA
ATTGCTCAGC
AAGAATTATC
ATTTGGAGAA
CGAAGTCGCC
AGGCTCAAGA
AAGGCTCGGG
GTCGGGGGAG
GAATTGTTGA
GTAAAAACTA
CCACTTGGAA
AATGAAGTCG
CCAGGCTCAA
AAAAGGGAGT
GGGAGCGGCG
AAGAGTTATT
GAGCAAAAAT
TACCACTTGG
AGAACGAAGT
GGCAAGGCTC
AAGAAAGGGA
GCGGCAGCGG
GGAGGAGCTC
TTATCGAAGA
ACTACCACTT
AGAGAATGAA
GTCGCCCGCT
TGAAGAAAGG
CTCGGGGAGC
GGGGAAGAGC
TCTTGAGCAA
GAACTACCAC
TTGGAAAATG
AGGTGGCGCG
CTTGAAGAAA
GGGAGCGGGA
GCGGGGAAGA
GTTACTATCT
AAGAATTATC
ATCTCGAGAA
CGAGGTGGCT
CGACTAAAGA
AGGGCTCCGG
CAGTGGGGAG
GAACTCCTGT
CGAAGAACTA
TCATCTTGAA
AATGAGGTTG
CAAGACTTAA
AAAGGGGTCC
GGATCAGGTG
AGGAACTACT
CAGTAAGAAT
TACCACCTGG
AAAACGAAGT
TGCACGTTTG
AAGAAAGGAT
CAGGATCAGG
CGAAGAACTG
CTCTCAAAAG
ATTATCATTT
GGAAAATGAG
GTTGCACGTT
TAAAAAAGGG
AAGTGGCAGT
GGTGAGGAAC
TTCTGTCGAA
AAATTATCAT
CTCGAGAATG
AAGTAGCCCG
ACTTAAAAAG
GGTTCTGGCT
CGGGTCAGCG
GCCGCAAGGT
GGAGGTGGAC
CCAAGAAGAA
GCGCAAGGTG
GGAAGCGGAG
CTACTAACTT
CAGCCTGCTG
AAGCAGGCTG
GAGACGTGGA
GGAGAACCCT
GGACCTGCAC
CAGGAAGTAT
GAGCGAGCTG
ATTAAGGAGA
ACATGCACAT
GAAGCTGTAC
ATGGAGGGCA
CCGTGGACAA
CCATCACTTC
AAGTGCACAT
CCGAGGGCGA
AGGCAAGCCC
TACGAGGGCA
CCCAGACCAT
GAGAATCAAG
GTGGTCGAGG
GCGGCCCTCT
CCCCTTCGCC
TTCGACATCC
TGGCTACTAG
CTTCCTCTAC
GGCAGCAAGA
CCTTCATCAA
CCACACCCAG
GGCATCCCCG
ACTTCTTCAA
GCAGTCCTTC
CCTGAGGGCT
TCACATGGGA
GAGAGTCACC
ACATACGAAG
ACGGGGGCGT
GCTGACCGCT
ACCCAGGACA
CCAGCCTCCA
GGACGGCTGC
CTCATCTACA
ACGTCAAGAT
CAGAGGGGTG
AACTTCACAT
CCAACGGCCC
TGTGATGCAG
AAGAAAACAC
TCGGCTGGGA
GGCCTTCACC
GAGACGCTGT
ACCCCGCTGA
CGGCGGCCTG
GAAGGCAGAA
ACGACATGGC
CCTGAAGCTC
GTGGGCGGGA
GCCATCTGAT
CGCAAACATC
AAGACCACAT
ATAGATCCAA
GAAACCCGCT
AAGAACCTCA
AGATGCCTGG
CGTCTACTAT
GTGGACTACA
GACTGGAAAG
AATCAAGGAG
GCCAACAACG
AGACCTACGT
CGAGCAGCAC
GAGGTGGCAG
TGGCCAGATA
CTGCGACCTC
CCTAGCAAAC
TGGGGCACAA
GCTTAATTAA
GCGGCCGCGA
CTCTAGAGTC
GACCTGCAGG
GATCGATCAC
TGAGGTACCT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGGGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AGACAAGATC
TGCTTTTTGC
TTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
T