名称: | pC003 - LshC2C2 locus into pACYC184 for spacer cloning |
---|---|
别称: | Plasmid#79152 |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 干扰沉默 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Chl |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | p15A |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pACYC184 |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGTATGGCAA
TGAAAGACGG
TGAGCTGGTG
ATATGGGATA
GTGTTCACCC
TTGTTACACC
GTTTTCCATG
AGCAAACTGA
AACGTTTTCA
TCGCTCTGGA
GTGAATACCA
CGACGATTTC
CGGCAGTTTC
TACACATATA
TTCGCAAGAT
GTGGCGTGTT
ACGGTGAAAA
CCTGGCCTAT
TTCCCTAAAG
GGTTTATTGA
GAATATGTTT
TTCGTCTCAG
CCAATCCCTG
GGTGAGTTTC
ACCAGTTTTG
ATTTAAACGT
GGCCAATATG
GACAACTTCT
TCGCCCCCGT
TTTCACCATG
GGCAAATATT
ATACGCAAGG
CGACAAGGTG
CTGATGCCGC
TGGCGATTCA
GGTTCATCAT
GCCGTCTGTG
ATGGCTTCCA
TGTCGGCAGA
ATGCTTAATG
AATTACAACA
GTACTGCGAT
GAGTGGCAGG
GCGGGGCGTA
ATTTTTTTAA
GGCAGTTATT
GGTGCCCTTA
AACGCCTGGT
GCTACGCCTG
AATAAGTGAT
AATAAGCGGA
TGAATGGCAG
AAATTCGAAA
GCAAATTCGA
CCCGGTCGTC
GGTTCAGGGC
AGGGTCGTTA
AATAGCCGCT
TATGTCTATT
GCTGGTTTAC
CGGTTTATTG
ACTACCGGAA
GCAGTGTGAC
CGTGTGCTTC
TCAAATGCCT
GAGGCCAGTT
TGCTCAGGCT
CTCCCCGTGG
AGGTAATAAT
TGACGATATG
ATCATTTATT
CTGCCTCCCA
GAGCCTGATA
AAAACGGTTA
GCGCTTCGTT
AATACAGATG
TAGGTGTTCC
ACAGGGTAGC
CAGCAGCATC
CTGCGATGCA
GATCCGGAAC
ATAATGGTGC
AGGGCGCTTG
TTTCGGCGTG
GGTATGGTGG
CAGGCCCCGT
GGCCGGGGGA
CTGTTGGGCG
CTGCCGGCAC
CTGTCCTACG
AGTTGCATGA
TAAAGAAGAC
AGTCATAAGT
GCGGCGACGA
TAGTCATGCC
CCGCGCCCAC
CGGAAGGAGC
TACCGGACAG
CGGTGCGGAC
TGTTGTAACT
CAGAATAAGA
AATGAGGCCG
CTCATGGCGT
TGACTCTCAG
TCATAGTATC
GTGGTATCAC
CGGTTGGTTC
CACTCTCTGT
TGCGGGCAAC
TTCAGCAGCA
CGTAGGGGAC
TTCCGCGTTT
CCAGACTTTA
CGAAACACGG
AAACCGAAGA
CCATTCATGT
TGTTGCTCAG
GTCGCAGACG
TTTTGCAGCA
GCAGTCGCTT
CACGTTCGCT
CGCGTATCGG
TGATTCATTC
TGCTAACCAG
TAAGGCAACC
CCGCCAGCCT
AGCCGGGTCC
TCAACGACAG
GAGCACGATC
ATGCGCACCC
GTGGCCAGGA
CCCAACGCTG
CCCGAGATGC
GCCGCGTGCG
GCTGCTGGAG
ATGGCGGACG
CGATGGATAT
GTTCTGCCAA
GGGTTGGTTT
GCGCATTCAC
AGTTCTCCGC
AAGAATTGAT
TGGCTCCAAT
TCTTGGAGTG
GTGAATCCGT
TAGCGAGGTG
CCGCCGGCTT
CCATTCAGGT
CGAGGTGGCC
CGGCTCCATG
CACCGCGACG
CAACGCGGGG
AGGCAGACAA
GGTATAGGGC
GGCGCCTACA
ATCCATGCCA
ACCCGTTCCA
TGTGCTCGCC
GAGGCGGCAT
AAATCGCCGT
GACGATCAGC
GGTCCAATGA
TCGAAGTTAG
GCTGGTAAGA
GCCGCGAGCG
ATCCTTGAAG
CTGTCCCTGA
TGGTCGTCAT
CTACCTGCCT
GGACAGCATG
GCCTGCAACG
CGGGCATCCC
GATGCCGCCG
GAAGCGAGAA
GAATCATAAT
GGGGAAGGCC
ATCCAGCCTC
GCGTCGCGAA
CGCCAGCAAG
ACGTAGCCCA
GCGCGTCGGC
CGCCATGCCG
GCGATAATGG
CCTGCTTCTC
GCCGAAACGT
TTGGTGGCGG
GACCAGTGAC
GAAGGCTTGA
GCGAGGGCGT
GCAAGATTCC
GAATACCGCA
AGCGACAGGC
CGATCATCGT
CGCGCTCCAG
CGAAAGCGGT
CCTCGCCGAA
AATGACCCAG
AGCGCTGCCG
GCACCTGTCC
TACGAGTTGC
ATGATAAAGA
AGACAGTCAT
AAGTGCGGCG
ACGATAGTCA
TGCCCCGCGC
CCACCGGAAG
GAGCTGACTG
GGTTGAAGGC
TCTCAAGGGC
ATCGGTCGAC
GCTCTCCCTT
ATGCGACTCC
TGCATTAGGA
AGCAGCCCAG
TAGTAGGTTG
AGGCCGTTGA
GCACCGCCGC
CGCAAGGAAT
GGTGCATGCA
AGGAGATGGC
GCCCAACAGT
CCCCCGGCCA
CGGGGCCTGC
CACCATACCC
ACGCCGAAAC
AAGCGCTCAT
GAGCCCGAAG
TGGCGAGCCC
GATCTTCCCC
ATCGGTGATG
TCGGCGATAT
AGGCGCCAGC
AACCGCACCT
GTGGCGCCGG
TGATGCCGGC
CACGATGCGT
CCGGCGTAGA
GGATCCACAG
GACGGGTGTG
GTCGCCATGA
TCGCGTAGTC
GATAGTGGCT
CCAAGTAGCG
AAGCGAGCAG
GACTGGGCGG
CGGCCAAAGC
GGTCGGACAG
TGCTCCGAGA
ACGGGTGCGC
ATAGAAATTG
CATCAACGCA
TATAGCGCTA
GCAGCACGCC
ATAGTGACTG
GCGATGCTGT
CGGAATGGAC
GATATCCCGC
AAGAGGCCCG
GCAGTACCGG
CATAACCAAG
CCTATGCCTA
CAGCATCCAG
GGTGACGGTG
CCGAGGATGA
CGATGAGCGC
ATTGTTAGAT
TTCATACACG
GTGCCTGACT
GCGTTAGCAA
TTTAACTGTG
ATAAACTACC
GCATTAAAGC
TCGTTTAACA
GCTGGGAAAA
TGTGGAATTT
GAAACAAATT
ATCAGGATGA
AAGATTTATT
GATTTGGTAA
CAGAAGGCAG
AACATTTAGA
ACAGGTTCAA
ATGGGAAAAT
TAAGTTGGAT
CTAAAGGCGA
AAGAAGGATT
GATTTTACGA
AAAGTAAGGA
TAGATGGGGA
TTATGAGTAA
ATAGAAGTAG
GGAGCTATGT
TTGACGGCTC
CTTATTTTTT
TGAGATTTAA
TATTGATGTG
GTATACTAAA
AATGGAAAAT
TGTATTTTTG
ATTAGAAAGA
TGTAAAATTG
ATTTAATTTA
AAAATATTTT
ATTAGATTAA
AGTAGAAACG
AGGAGATTGG
AATGGGAAAT
TTATTTGGAC
ATAAGAGATG
GTATGAAGTT
CGTGATAAAA
AAGATTTTAA
AATAAAACGG
AAAGTAAAAG
TTAAAAGGAA
CTATGATGGA
AACAAATACA
TTTTAAATAT
TAATGAAAAT
AATAATAAAG
AAAAAATTGA
TAATAATAAA
TTTATTAGAA
AATATATTAA
TTACAAAAAA
AATGATAATA
TTCTTAAAGA
ATTTACAAGA
AAATTTCATG
CAGGAAATAT
TCTATTTAAG
TTGAAAGGTA
AAGAAGGAAT
CATAAGAATA
GAAAATAATG
ATGATTTTTT
GGAAACAGAA
GAAGTTGTAT
TATATATAGA
AGCATATGGA
AAATCTGAGA
AATTGAAAGC
ATTGGGGATT
ACAAAAAAGA
AAATAATAGA
TGAAGCAATT
AGGCAAGGAA
TAACTAAAGA
TGATAAAAAA
ATAGAAATAA
AGAGACAAGA
GAATGAAGAA
GAAATAGAGA
TAGATATCAG
AGATGAGTAT
ACTAATAAAA
CACTAAATGA
TTGTTCAATA
ATATTAAGAA
TAATAGAAAA
TGATGAATTA
GAAACAAAAA
AATCAATTTA
CGAAATTTTT
AAAAATATTA
ACATGAGTTT
ATATAAGATT
ATAGAAAAAA
TAATTGAAAA
TGAAACAGAA
AAAGTATTTG
AAAATCGATA
TTATGAAGAA
CATTTAAGAG
AAAAATTATT
GAAAGATGAT
AAAATAGATG
TTATTTTGAC
TAATTTTATG
GAAATAAGAG
AAAAAATAAA
AAGTAATTTA
GAAATTCTGG
GTTTTGTAAA
ATTTTATCTT
AATGTTGGTG
GTGATAAAAA
GAAATCTAAA
AATAAAAAAA
TGCTTGTTGA
AAAAATTTTA
AATATAAATG
TAGATTTAAC
AGTAGAAGAT
ATAGCTGATT
TTGTTATAAA
AGAGTTAGAA
TTTTGGAATA
TTACTAAAAG
AATTGAAAAA
GTAAAAAAAG
TTAATAATGA
ATTTCTTGAA
AAGAGAAGAA
ATAGAACATA
TATAAAATCT
TATGTACTAT
TAGATAAGCA
CGAAAAATTT
AAAATAGAAA
GAGAAAATAA
AAAAGATAAA
ATTGTAAAAT
TTTTTGTAGA
AAATATAAAA
AATAATAGTA
TAAAAGAAAA
AATAGAAAAA
ATTTTAGCAG
AATTTAAAAT
AGATGAATTA
ATAAAAAAAT
TAGAAAAAGA
ACTAAAAAAA
GGAAATTGTG
ATACAGAAAT
TTTTGGAATA
TTTAAAAAAC
ATTATAAAGT
TAATTTTGAT
TCGAAAAAAT
TTTCAAAGAA
ATCTGATGAA
GAAAAAGAAC
TATATAAAAT
CATATATCGA
TATTTGAAAG
GAAGAATTGA
AAAAATATTG
GTAAATGAAC
AAAAAGTCAG
ATTAAAGAAA
ATGGAAAAAA
TAGAAATAGA
AAAAATTTTA
AATGAAAGTA
TATTATCTGA
AAAAATTTTA
AAAAGAGTTA
AGCAATATAC
GTTAGAGCAT
ATTATGTATT
TAGGGAAATT
AAGACATAAT
GATATAGATA
TGACAACAGT
AAATACTGAT
GATTTTTCTA
GACTTCACGC
TAAAGAGGAA
TTGGATTTAG
AATTGATTAC
TTTTTTTGCT
TCTACCAATA
TGGAATTAAA
TAAGATTTTT
TCTAGAGAAA
ATATAAATAA
TGATGAAAAT
ATAGATTTCT
TTGGAGGAGA
TCGAGAAAAA
AATTACGTAC
TTGATAAAAA
AATTTTAAAT
TCAAAAATAA
AAATAATAAG
AGACTTAGAT
TTTATAGATA
ATAAAAATAA
CATTACAAAT
AATTTTATTA
GGAAATTTAC
AAAGATAGGA
ACAAATGAAA
GAAACAGGAT
ATTACATGCG
ATTAGCAAGG
AAAGAGATTT
ACAAGGAACG
CAAGATGACT
ATAATAAAGT
CATAAATATA
ATTCAAAATT
TAAAAATATC
AGATGAAGAA
GTGTCAAAAG
CATTAAATTT
AGATGTAGTA
TTTAAAGATA
AAAAAAATAT
AATTACTAAA
ATAAATGATA
TTAAAATTTC
AGAAGAAAAT
AATAATGATA
TTAAATATTT
ACCATCCTTT
TCTAAAGTAC
TTCCTGAAAT
ATTAAATTTA
TATAGGAATA
ATCCTAAGAA
TGAACCATTT
GATACTATAG
AAACAGAAAA
AATAGTATTA
AATGCTTTAA
TATATGTGAA
TAAAGAATTG
TATAAGAAAT
TAATTTTAGA
AGATGATTTA
GAAGAAAATG
AAAGTAAGAA
TATATTTTTA
CAAGAATTAA
AGAAAACTTT
AGGAAACATT
GATGAAATAG
ATGAAAATAT
TATAGAAAAT
TATTATAAAA
ATGCTCAGAT
TTCTGCTTCA
AAAGGTAATA
ATAAAGCTAT
TAAAAAATAT
CAGAAAAAAG
TAATAGAATG
TTATATTGGA
TATTTGAGAA
AAAATTATGA
GGAACTTTTT
GATTTTTCAG
ATTTTAAAAT
GAATATTCAA
GAAATTAAGA
AACAAATAAA
AGACATTAAT
GATAATAAAA
CTTACGAAAG
AATAACTGTA
AAGACTAGTG
ATAAAACTAT
AGTAATAAAT
GATGATTTTG
AATATATAAT
TTCAATATTT
GCACTGTTAA
ATAGTAATGC
GGTTATAAAT
AAAATTAGAA
ATAGATTTTT
TGCAACATCA
GTTTGGTTAA
ATACTTCAGA
ATATCAAAAT
ATTATAGATA
TTTTAGATGA
AATAATGCAA
TTAAATACTT
TGAGAAATGA
ATGTATAACT
GAAAATTGGA
ATCTTAATTT
AGAAGAATTT
ATTCAAAAAA
TGAAAGAGAT
AGAAAAAGAC
TTTGATGATT
TTAAAATACA
AACTAAAAAA
GAAATTTTTA
ATAATTATTA
TGAGGATATA
AAAAATAATA
TATTAACTGA
ATTTAAAGAT
GATATTAATG
GTTGTGATGT
TTTAGAGAAA
AAATTAGAAA
AAATTGTAAT
TTTTGATGAT
GAAACTAAAT
TTGAGATAGA
TAAAAAATCG
AATATATTAC
AAGATGAACA
AAGAAAGTTA
TCAAACATAA
ATAAAAAGGA
TTTGAAAAAA
AAGGTTGATC
AGTATATAAA
AGATAAAGAT
CAGGAAATAA
AGAGTAAAAT
ATTGTGTAGA
ATAATATTTA
ATTCTGATTT
TTTAAAAAAA
TACAAAAAAG
AAATAGATAA
TTTAATAGAA
GATATGGAAT
CAGAGAATGA
AAATAAATTT
CAAGAAATAT
ATTATCCTAA
AGAACGTAAG
AATGAATTAT
ATATTTATAA
AAAGAATTTA
TTTTTAAATA
TTGGAAATCC
AAATTTTGAT
AAAATTTATG
GATTAATTTC
AAATGATATT
AAAATGGCTG
ATGCAAAATT
TTTATTTAAT
ATTGATGGTA
AAAATATTAG
AAAAAATAAA
ATATCTGAAA
TTGATGCAAT
ATTGAAAAAT
CTAAATGATA
AACTAAATGG
ATATTCTAAA
GAATACAAAG
AGAAGTATAT
AAAAAAATTG
AAAGAAAATG
ATGACTTTTT
TGCAAAAAAT
ATACAAAATA
AAAACTATAA
ATCATTTGAA
AAAGATTATA
ATAGAGTTTC
TGAATACAAA
AAAATTAGAG
ATTTGGTAGA
ATTTAATTAT
TTGAATAAAA
TAGAAAGTTA
TTTGATAGAT
ATAAATTGGA
AACTTGCTAT
TCAAATGGCT
AGATTTGAAA
GAGATATGCA
CTATATTGTA
AATGGGCTAA
GAGAATTAGG
AATTATTAAG
TTAAGTGGCT
ACAATACTGG
AATAAGTAGA
GCTTATCCTA
AGCGTAATGG
ATCTGATGGC
TTTTATACAA
CAACAGCATA
CTATAAATTT
TTTGATGAAG
AATCTTATAA
AAAATTTGAG
AAAATATGTT
ATGGATTTGG
AATTGATTTG
TCTGAAAATT
CTGAAATTAA
TAAGCCGGAA
AATGAAAGTA
TTAGAAACTA
TATTTCACAT
TTCTATATTG
TAAGAAATCC
GTTTGCTGAT
TACAGTATTG
CAGAACAAAT
TGATAGAGTT
TCTAATTTAT
TATCATATAG
TACACGTTAT
AATAATTCAA
CTTATGCAAG
TGTATTTGAA
GTATTTAAAA
AAGATGTGAA
TTTAGATTAT
GATGAATTAA
AGAAAAAATT
TAAACTTATC
GGTAATAATG
ATATACTTGA
AAGATTAATG
AAACCTAAAA
AAGTTTCTGT
TTTAGAATTA
GAAAGTTATA
ATTCTGATTA
TATTAAAAAC
TTGATAATAG
AACTTTTAAC
AAAAATAGAA
AATACGAATG
ATACGTTATA
AGAATGAAAT
TTATAAGGAG
TATGATTAAA
ATGCCCAATA
TAATTCACAT
AACAAGGGCT
GATGATTTGT
CAATATCGAA
TAATCAGCTT
ATAATGGTTG
ATGAAGACAA
TAATGATGAG
AAAAATAAGA
TTTCTTTGAA
TGATATATCT
GCGATAGTTA
TTGAAAACTG
CTATTGTAAG
ATTAGTGCGA
TTTTGCAGTT
GAGGCTGGTG
GAAAATAACA
TTCCGATTAT
TATTTGCAAC
GAGAAACATC
AGCCTGAAAT
TCATTCGCTG
GGATTATTTA
ATCATTTTCA
AGTGACTTTA
CGAATAAATG
AGCAGATTGA
GTGGGAAAAA
GAGAAAAAGG
AAAAATTGTG
GAGCAGGATA
GTGGAGAATA
AGATTGAAAA
TCAAAGAGCA
TTGTTAGAGT
ATCTTGAAAA
AAGTGATATT
TCCATTGAAA
GACTGAAAAC
ATATAAGGAA
AACTTGAAAA
AAGATGATGC
GAGTGCAGAA
CATCAGGAAG
CAATAGCTTC
AAGGATATAT
TTTCAGGAAT
TGTATAGTAA
TAGTTTTAAA
AGATTTGATG
AAGATGGAGT
GAATTCTGCA
CTTAATTATG
GATATATGAT
TTTAAGAGCT
ATAATATCAT
CAAAGATTGT
AGCAAAAGGA
TTTCATCCAA
GTTTAGGATT
ACATCATAAG
TCACAGTTTA
ATGCCTATAA
TTTTTCTGAT
GATATAATAG
AGGTTTTTCG
TCCTATGGTG
GATTATCTTG
TTTATATGTA
CAAGGATATT
TTGAATGAAG
TGAAATTAAG
TAAGGAAATA
AGGCAAAAAA
TACTTCTTGT
AGCACAGCAG
AAGGTATTAT
TTAACAATAA
GAAATATGAT
TTTTTCCAAG
CGGTAGACTA
TTATTTGGAT
AGTATACGAA
ATTATTTTGT
AAAAGATGAA
GAAGTGATAA
TTCCGACATT
GAGCGTGATG
GACTATGAGT
ATTGAGTATT
TTGGTCTGCT
TATGTATGAT
TTTCCTATGC
AGACAGATGT
TGAGAGATTT
GAATATAGGA
CTTTTAGGAA
AAAATTGATA
GAAAAGGGAT
ATTATCAGCT
TCAAAAATCG
GTATATATAA
TGAGGTCGAA
AACAAAAGAA
AAAATCGAAC
TGGCAGAAAA
ACAGTTATCA
ATGCTCGCTC
CAGAAAATTC
ATCAATCCGT
TCCATTATCC
TGACAGAAGA
TCAGTTTCAA
AAATGAAGGT
ACTTGCAGGA
GAAGTGACAA
TGGGAGAAAA
AATTTCACAA
AACAAAATAT
TGGAATTTTG
AAAAATCAAA
AAATATGGTA
TAATAGTATA
GGGTGGTTCT
ATATTATTAA
TAAAGAGAAA
AATAGGATTG
AAAGTATTGA
AAATAAAGAC
AAAATAGAAA
AATTAGAAAT
AAATATCTCA
TCAGCAAGAA
ATTAGGATGG
GATATAGACC
ACCCCAATAT
CGAAGGGGAC
TAAAACGGAG
ACCGAGATTG
GTCTCGGATA
TAGACCACCC
CAATATCGAA
GGGGACTAAA
ACTCTAAAGA
ATTATCTATT
CTGTCTTTTA
AATTGATATA
GACCACCCCA
ATATCGAAAA
GTGATATTTA
ATAAAAATAA
CTTCTGAGTG
AGAATAAAAT
TTCAATTCTT
GCTCATTTTT
TATTGTTTTT
TGAATATGGT
TGCTTGGTTG
TTCTGAAACG
AAAATTTTTG
GAGATGTTTT
AAATTTTTAG
GTTGAAAAAA
AATAAGAATT
ATACTATAAT
AAATAATTAT
GCGATTAGGC
TAACTAGAGC
CTAATTTAAA
TATTTTATCT
GATTAATAAG
ATGATCTTCT
TGAGATCGTT
TTGGTCTGCG
CGTAATCTCT
TGCTCTGAAA
ACGAAAAAAC
CGCCTTGCAG
GGCGGTTTTT
CGAAGGTTCT
CTGAGCTACC
AACTCTTTGA
ACCGAGGTAA
CTGGCTTGGA
GGAGCGCAGT
CACCAAAACT
TGTCCTTTCA
GTTTAGCCTT
AACCGGCGCA
TGACTTCAAG
ACTAACTCCT
CTAAATCAAT
TACCAGTGGC
TGCTGCCAGT
GGTGCTTTTG
CATGTCTTTC
CGGGTTGGAC
TCAAGACGAT
AGTTACCGGA
TAAGGCGCAG
CGGTCGGACT
GAACGGGGGG
TTCGTGCATA
CAGTCCAGCT
TGGAGCGAAC
TGCCTACCCG
GAACTGAGTG
TCAGGCGTGG
AATGAGACAA
ACGCGGCCAT
AACAGCGGAA
TGACACCGGT
AAACCGAAAG
GCAGGAACAG
GAGAGCGCAC
GAGGGAGCCG
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCA
CTGATTTGAG
CGTCAGATTT
CGTGATGCTT
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGG
CTTTGCCGCG
GCCCTCTCAC
TTCCCTGTTA
AGTATCTTCC
TGGCATCTTC
CAGGAAATCT
CCGCCCCGTT
CGTAAGCCAT
TTCCGCTCGC
CGCAGTCGAA
CGACCGAGCG
TAGCGAGTCA
GTGAGCGAGG
AAGCGGAATA
TATCCTGTAT
CACATATTCT
GCTGACGCAC
CGGTGCAGCC
TTTTTTCTCC
TGCCACATGA
AGCACTTCAC
TGACACCCTC
ATCAGTGCCA
ACATAGTAAG
CCAGTATACA
CTCCGCTAGC
GCTGATGTCC
GGCGGTGCTT
TTGCCGTTAC
GCACCACCCC
GTCAGTAGCT
GAACAGGAGG
GACAGCTGAT
AGAAACAGAA
GCCACTGGAG
CACCTCAAAA
ACACCATCAT
ACACTAAATC
AGTAAGTTGG
CAGCATCACC
CGACGCACTT
TGCGCCGAAT
AAATACCTGT
GACGGAAGAT
CACTTCGCAG
AATAAATAAA
TCCTGGTGTC
CCTGTTGATA
CCGGGAAGCC
CTGGGCCAAC
TTTTGGCGAA
AATGAGACGT
TGATCGGCAC
GTAAGAGGTT
CCAACTTTCA
CCATAATGAA
ATAAGATCAC
TACCGGGCGT
ATTTTTTGAG
TTATCGAGAT
TTTCAGGAGC
TAAGGAAGCT
AAAATGGAGA
AAAAAATCAC
TGGATATACC
ACCGTTGATA
TATCCCAATG
GCATCGTAAA
GAACATTTTG
AGGCATTTCA
GTCAGTTGCT
CAATGTACCT
ATAACCAGAC
CGTTCAGCTG
GATATTACGG
CCTTTTTAAA
GACCGTAAAG
AAAAATAAGC
ACAAGTTTTA
TCCGGCCTTT
ATTCACATTC
TTGCCCGCCT
GATGAATGCT
CATCCGGAAT
TC