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pCS2-nCas9n

产品货号:P0747

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCS2-nCas9n
别称: Plasmid#47929
基因长:
质粒宿主: 哺乳细胞,鱼类
质粒用途: 基因编辑
片段类型: CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体: pCS2+
正向引物: CMV-F:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CGCCATTCTG

CCTGGGGACG

TCGGAGCAAG

CTTGATTTAG

GTGACACTAT

AGAATACAAG

61

CTACTTGTTC

TTTTTGCAGG

ATCCGATCCA

ATGTCGACGC

CACCATGGCT

TCTCCACCTA

121

AGAAGAAGAG

AAAGGTGGGA

AGCATGGATA

AGAAGTATAG

CATCGGCCTG

GATATTGGAA

181

CTAACTCCGT

GGGTTGGGCA

GTGATTACAG

ACGACTACAA

GGTCCCTAGC

AAGAAATTTA

241

AGGTGCTGGG

TAACACCGAC

AGGCACAGCA

TCAAGAAAAA

TCTGATTGGA

GCCCTGCTGT

301

TCGGTTCTGG

AGAGACTGCC

GAAGCAACAC

GCCTGAAAAG

AACAGCAAGA

AGGCGCTATA

361

CCAGAAGGAA

GAATAGAATC

TGTTACCTGC

AGGAGATTTT

CTCTAACGAA

ATGGCTAAGG

421

TGGACGATTC

ATTCTTTCAT

AGGCTGGAGG

AAAGTTTCCT

GGTCGAGGAA

GATAAGAAAC

481

ACGAGCGCCA

TCCTATCTTT

GGAAACATTG

TGGACGAGGT

CGCCTATCAC

GAAAAATACC

541

CAACCATCTA

TCATCTGCGC

AAGAAACTGG

CTGACTCTAC

TGATAAAGCC

GACCTGAGAC

601

TGATCTATCT

GGCTCTGGCC

CACATGATTA

AGTTCAGGGG

TCATTTTCTG

ATCGAGGGCG

661

ATCTGAACCC

CGACAATTCC

GATGTGGACA

AGCTGTTCAT

CCAGCTGGTC

CAGATTTACA

721

ATCAGCTGTT

TGAGGAAAAC

CCTATTAATG

CTTCCAGAGT

GGACGCAAAA

GCTATCCTGT

781

CAGCCAGGCT

GTCCAAGTCA

CGCAGACTGG

AGAACCTGAT

TGCACAGCTG

CCCGGAGAAA

841

AGAGGAACGG

TCTGTTTGGA

AATCTGATCG

CTCTGAGTCT

GGGCCTGACT

CCTAACTTCA

901

AAAGCAATTT

TGATCTGGCT

GAGGACGCCA

AACTGCAGCT

GTCAAAGGAC

ACATATGACG

961

ATGACCTGGA

TAACCTGCTG

GCACAGATCG

GAGATCAGTA

CGCTGACCTG

TTCCTGGCTG

1021

CCAAAAATCT

GTCCGACGCA

ATCCTGCTGT

CAGATATTCT

GAGAGTGAAC

AGCGAGATTA

1081

CAAAAGCTCC

TCTGAGTGCC

AGCATGATCA

AGAGATATGA

CGAGCACCAT

CAGGATCTGA

1141

CCCTGCTGAA

GGCTCTGGTC

AGGCAGCAGC

TGCCAGAGAA

GTACAAGGAA

ATTTTCTTTG

1201

ATCAGTCCAA

GAACGGCTAC

GCCGGTTATA

TCGACGGAGG

CGCATCACAG

GAGGAATTCT

1261

ACAAGTTTAT

CAAACCTATT

CTGGAGAAGA

TGGACGGAAC

TGAGGAACTG

CTGGTGAAAC

1321

TGAATAGAGA

GGACCTGCTG

AGGAAGCAGC

GCACATTTGA

TAACGGTTCC

ATCCCACACC

1381

AGATTCATCT

GGGAGAGCTG

CACGCTATCC

TGAGGCGCCA

GGAAGACTTC

TACCCCTTTC

1441

TGAAAGATAA

CCGCGAGAAG

ATCGAAAAAA

TTCTGACCTT

CAGAATCCCT

TACTATGTGG

1501

GTCCACTGGC

TCGCGGAAAC

AGCAGATTTG

CCTGGATGAC

TCGCAAATCC

GAGGAAACCA

1561

TTACTCCTTG

GAACTTCGAG

GAAGTGGTCG

ATAAGGGCGC

CTCTGCACAG

TCCTTCATCG

1621

AGAGAATGAC

TAATTTTGAC

AAAAACCTGC

CCAATGAGAA

AGTGCTGCCT

AAGCACTCCC

1681

TGCTGTACGA

GTATTTCACT

GTCTATAACG

AACTGACAAA

GGTGAAATAC

GTCACCGAGG

1741

GCATGAGAAA

GCCAGCCTTC

CTGTCAGGAG

AGCAGAAGAA

AGCAATCGTG

GATCTGCTGT

1801

TTAAAACCAA

CAGGAAAGTG

ACTGTCAAGC

AGCTGAAGGA

GGACTACTTC

AAGAAAATTG

1861

AATGCTTCGA

TTCCGTGGAG

ATCAGCGGAG

TCGAAGACAG

ATTTAACGCA

AGCCTGGGCG

1921

CTTACCACGA

TCTGCTGAAG

ATCATTAAGG

ATAAAGACTT

CCTGGACAAC

GAGGAAAATG

1981

AGGATATCCT

GGAAGACATT

GTGCTGACAC

TGACCCTGTT

TGAGGACAGA

GGAATGATCG

2041

AGGAAAGACT

GAAAACCTAT

GCTCATCTGT

TCGATGACAA

GGTGATGAAA

CAGCTGAAGA

2101

GAAGGCGCTA

CACTGGCTGG

GGTAGACTGA

GCAGGAAGCT

GATCAACGGC

ATTAGGGATA

2161

AACAGTCAGG

AAAGACAATC

CTGGACTTTC

TGAAAAGTGA

TGGCTTCGCC

AACCGCAATT

2221

TTATGCAGCT

GATTCACGAT

GACAGTCTGA

CCTTCAAAGA

GGACATCCAG

AAGGCTCAGG

2281

TGTCTGGACA

GGGCCACTCC

CTGCATGAGC

AGATTGCAAA

CCTGGCTGGA

AGCCCAGCCA

2341

TCAAGAAAGG

CATTCTGCAG

ACAGTGAAAA

TCGTCGATGA

GCTGGTGAAA

GTCATGGGCC

2401

ATAAGCCCGA

AAACATCGTG

ATTGAGATGG

CTCGCGAAAA

TCAGACAACC

CAGAAGGGTC

2461

AGAAGAACAG

TAGAGAGAGG

ATGAAAAGAA

TCGAGGAAGG

CATTAAGGAG

CTGGGTAGCC

2521

AGATCCTGAA

AGAGCACCCA

GTGGAAAACA

CACAGCTGCA

GAATGAGAAG

CTGTATCTGT

2581

ACTATCTGCA

GAATGGAAGA

GATATGTACG

TGGACCAGGA

GCTGGATATT

AACAGGCTGT

2641

CTGATTACGA

CGTGGATCAT

ATCGTCCCCC

AGAGTTTCAT

CAAAGATGAC

AGCATTGACA

2701

ACAAGGTGCT

GACCAGGTCC

GACAAAAACA

GAGGAAAATC

AGATAATGTC

CCTAGTGAGG

2761

AAGTGGTCAA

GAAAATGAAG

AACTACTGGA

GACAGCTGCT

GAATGCCAAA

CTGATCACTC

2821

AGAGGAAGTT

TGATAACCTG

ACAAAAGCAG

AGCGCGGTGG

ACTGTCAGAA

CTGGACAAAG

2881

CTGGATTCAT

CAAGAGGCAG

CTGGTGGAAA

CACGCCAGAT

CACTAAACAC

GTCGCACAGA

2941

TTCTGGATAG

TCGCATGAAC

ACAAAGTACG

ATGAGAATGA

CAAACTGATC

AGAGAAGTGA

3001

AGGTCATTAC

CCTGAAGAGT

AAACTGGTCA

GCGACTTTAG

GAAAGATTTC

CAGTTTTATA

3061

AGGTCCGCGA

GATTAACAAT

TATCACCATG

CCCATGACGC

ATACCTGAAC

GCCGTGGTCG

3121

GTACCGCACT

GATCAAGAAA

TACCCAAAAC

TGGAGAGCGA

ATTCGTGTAC

GGAGACTATA

3181

AGGTGTACGA

TGTCAGAAAA

ATGATCGCCA

AGTCCGAGCA

GGAAATTGGA

AAAGCTACTG

3241

CCAAGTATTT

CTTTTACTCA

AACATCATGA

ATTTCTTTAA

GACAGAGATC

ACCCTGGCCA

3301

ATGGAGAAAT

CCGCAAAAGG

CCCCTGATTG

AGACAAACGG

AGAGACAGGC

GAAATCGTGT

3361

GGGACAAAGG

CAGAGATTTT

GCAACCGTGA

GGAAGGTCCT

GAGCATGCCT

CAAGTGAATA

3421

TCGTCAAGAA

AACTGAGGTG

CAGACAGGCG

GTTTCTCAAA

AGAAAGTATT

CTGCCAAAAC

3481

GCAACTCTGA

TAAGCTGATC

GCTAGAAAGA

AAGACTGGGA

CCCTAAGAAG

TATGGAGGCT

3541

TTGACTCTCC

CACTGTGGCA

TACTCCGTCC

TGGTGGTCGC

TAAGGTGGAG

AAGGGCAAAA

3601

GCAAGAAACT

GAAATCTGTC

AAGGAGCTGC

TGGGTATCAC

AATTATGGAG

AGAAGCTCTT

3661

TCGAGAAGAA

CCCAATCGAT

TTTCTGGAGG

CCAAAGGTTA

TAAGGAAGTG

AAGAAAGACC

3721

TGATCATTAA

ACTGCCCAAG

TACAGTCTGT

TTGAGCTGGA

AAACGGCAGG

AAACGCATGC

3781

TGGCAAGCGC

TGGAGAGCTG

CAGAAAGGCA

ATGAACTGGC

CCTGCCTTCT

AAGTACGTGA

3841

ACTTCCTGTA

TCTGGCAAGC

CACTACGAGA

AGCTGAAAGG

ATCTCCAGAG

GATAACGAAC

3901

AGAAACAGCT

GTTTGTGGAG

CAGCACAAGC

ATTATCTGGA

CGAGATCATT

GAACAGATTA

3961

GCGAGTTCTC

TAAAAGAGTG

ATCCTGGCCG

ACGCAAATCT

GGATAAGGTC

CTGTCTGCTT

4021

ACAACAAACA

CAGAGATAAG

CCCATCAGGG

AGCAGGCCGA

AAATATCATT

CATCTGTTCA

4081

CTCTGACAAA

CCTGGGCGCA

CCTGCAGCTT

TCAAGTACTT

CGACACTACA

ATCGATAGAA

4141

AGAGGTACAC

CTCCACTAAG

GAGGTGCTGG

ACGCTACACT

GATCCATCAG

AGTATTACCG

4201

GCCTGTACGA

AACAAGGATT

GACCTGTCTC

AGCTGGGTGG

CGACTCACCG

GTGAGATCTC

4261

CTAAGAAGAA

GAGAAAGGTG

TGACCGCGGA

ATCTAGAACT

ATAGTGAGTC

GTATTACGTA

4321

GATCCAGACA

TGATAAGATA

CATTGATGAG

TTTGGACAAA

CCACAACTAG

AATGCAGTGA

4381

AAAAAATGCT

TTATTTGTGA

AATTTGTGAT

GCTATTGCTT

TATTTGTAAC

CATTATAAGC

4441

TGCAATAAAC

AAGTTAACAA

CAACAATTGC

ATTCATTTTA

TGTTTCAGGT

TCAGGGGGAG

4501

GTGTGGGAGG

TTTTTTAATT

CGCGGCCGCG

GCGCCAATGC

ATTGGGCCCG

GTACCCAGCT

4561

TTTGTTCCCT

TTAGTGAGGG

TTAATTGCGC

GCTTGGCGTA

ATCATGGTCA

TAGCTGTTTC

4621

CTGTGTGAAA

TTGTTATCCG

CTCACAATTC

CACACAACAT

ACGAGCCGGA

AGCATAAAGT

4681

GTAAAGCCTG

GGGTGCCTAA

TGAGTGAGCT

AACTCACATT

AATTGCGTTG

CGCTCACTGC

4741

CCGCTTTCCA

GTCGGGAAAC

CTGTCGTGCC

AGCTGCATTA

ATGAATCGGC

CAACGCGCGG

4801

GGAGAGGCGG

TTTGCGTATT

GGGCGCTCTT

CCGCTTCCTC

GCTCACTGAC

TCGCTGCGCT

4861

CGGTCGTTCG

GCTGCGGCGA

GCGGTATCAG

CTCACTCAAA

GGCGGTAATA

CGGTTATCCA

4921

CAGAATCAGG

GGATAACGCA

GGAAAGAACA

TGTGAGCAAA

AGGCCAGCAA

AAGGCCAGGA

4981

ACCGTAAAAA

GGCCGCGTTG

CTGGCGTTTT

TCCATAGGCT

CCGCCCCCCT

GACGAGCATC

5041

ACAAAAATCG

ACGCTCAAGT

CAGAGGTGGC

GAAACCCGAC

AGGACTATAA

AGATACCAGG

5101

CGTTTCCCCC

TGGAAGCTCC

CTCGTGCGCT

CTCCTGTTCC

GACCCTGCCG

CTTACCGGAT

5161

ACCTGTCCGC

CTTTCTCCCT

TCGGGAAGCG

TGGCGCTTTC

TCATAGCTCA

CGCTGTAGGT

5221

ATCTCAGTTC

GGTGTAGGTC

GTTCGCTCCA

AGCTGGGCTG

TGTGCACGAA

CCCCCCGTTC

5281

AGCCCGACCG

CTGCGCCTTA

TCCGGTAACT

ATCGTCTTGA

GTCCAACCCG

GTAAGACACG

5341

ACTTATCGCC

ACTGGCAGCA

GCCACTGGTA

ACAGGATTAG

CAGAGCGAGG

TATGTAGGCG

5401

GTGCTACAGA

GTTCTTGAAG

TGGTGGCCTA

ACTACGGCTA

CACTAGAAGG

ACAGTATTTG

5461

GTATCTGCGC

TCTGCTGAAG

CCAGTTACCT

TCGGAAAAAG

AGTTGGTAGC

TCTTGATCCG

5521

GCAAACAAAC

CACCGCTGGT

AGCGGTGGTT

TTTTTGTTTG

CAAGCAGCAG

ATTACGCGCA

5581

GAAAAAAAGG

ATCTCAAGAA

GATCCTTTGA

TCTTTTCTAC

GGGGTCTGAC

GCTCAGTGGA

5641

ACGAAAACTC

ACGTTAAGGG

ATTTTGGTCA

TGAGATTATC

AAAAAGGATC

TTCACCTAGA

5701

TCCTTTTAAA

TTAAAAATGA

AGTTTTAAAT

CAATCTAAAG

TATATATGAG

TAAACTTGGT

5761

CTGACAGTTA

CCAATGCTTA

ATCAGTGAGG

CACCTATCTC

AGCGATCTGT

CTATTTCGTT

5821

CATCCATAGT

TGCCTGACTC

CCCGTCGTGT

AGATAACTAC

GATACGGGAG

GGCTTACCAT

5881

CTGGCCCCAG

TGCTGCAATG

ATACCGCGAG

ACCCACGCTC

ACCGGCTCCA

GATTTATCAG

5941

CAATAAACCA

GCCAGCCGGA

AGGGCCGAGC

GCAGAAGTGG

TCCTGCAACT

TTATCCGCCT

6001

CCATCCAGTC

TATTAATTGT

TGCCGGGAAG

CTAGAGTAAG

TAGTTCGCCA

GTTAATAGTT

6061

TGCGCAACGT

TGTTGCCATT

GCTACAGGCA

TCGTGGTGTC

ACGCTCGTCG

TTTGGTATGG

6121

CTTCATTCAG

CTCCGGTTCC

CAACGATCAA

GGCGAGTTAC

ATGATCCCCC

ATGTTGTGCA

6181

AAAAAGCGGT

TAGCTCCTTC

GGTCCTCCGA

TCGTTGTCAG

AAGTAAGTTG

GCCGCAGTGT

6241

TATCACTCAT

GGTTATGGCA

GCACTGCATA

ATTCTCTTAC

TGTCATGCCA

TCCGTAAGAT

6301

GCTTTTCTGT

GACTGGTGAG

TACTCAACCA

AGTCATTCTG

AGAATAGTGT

ATGCGGCGAC

6361

CGAGTTGCTC

TTGCCCGGCG

TCAATACGGG

ATAATACCGC

GCCACATAGC

AGAACTTTAA

6421

AAGTGCTCAT

CATTGGAAAA

CGTTCTTCGG

GGCGAAAACT

CTCAAGGATC

TTACCGCTGT

6481

TGAGATCCAG

TTCGATGTAA

CCCACTCGTG

CACCCAACTG

ATCTTCAGCA

TCTTTTACTT

6541

TCACCAGCGT

TTCTGGGTGA

GCAAAAACAG

GAAGGCAAAA

TGCCGCAAAA

AAGGGAATAA

6601

GGGCGACACG

GAAATGTTGA

ATACTCATAC

TCTTCCTTTT

TCAATATTAT

TGAAGCATTT

6661

ATCAGGGTTA

TTGTCTCATG

AGCGGATACA

TATTTGAATG

TATTTAGAAA

AATAAACAAA

6721

TAGGGGTTCC

GCGCACATTT

CCCCGAAAAG

TGCCACCTAA

ATTGTAAGCG

TTAATATTTT

6781

GTTAAAATTC

GCGTTAAATT

TTTGTTAAAT

CAGCTCATTT

TTTAACCAAT

AGGCCGAAAT

6841

CGGCAAAATC

CCTTATAAAT

CAAAAGAATA

GACCGAGATA

GGGTTGAGTG

TTGTTCCAGT

6901

TTGGAACAAG

AGTCCACTAT

TAAAGAACGT

GGACTCCAAC

GTCAAAGGGC

GAAAAACCGT

6961

CTATCAGGGC

GATGGCCCAC

TACGTGAACC

ATCACCCTAA

TCAAGTTTTT

TGGGGTCGAG

7021

GTGCCGTAAA

GCACTAAATC

GGAACCCTAA

AGGGAGCCCC

CGATTTAGAG

CTTGACGGGG

7081

AAAGCCGGCG

AACGTGGCGA

GAAAGGAAGG

GAAGAAAGCG

AAAGGAGCGG

GCGCTAGGGC

7141

GCTGGCAAGT

GTAGCGGTCA

CGCTGCGCGT

AACCACCACA

CCCGCCGCGC

TTAATGCGCC

7201

GCTACAGGGC

GCGTCCCATT

CGCCATTCAG

GCTGCGCAAC

TGTTGGGAAG

GGCGATCGGT

7261

GCGGGCCTCT

TCGCTATTAC

GCCAGTCGAC

CATAGCCAAT

TCAATATGGC

GTATATGGAC

7321

TCATGCCAAT

TCAATATGGT

GGATCTGGAC

CTGTGCCAAT

TCAATATGGC

GTATATGGAC

7381

TCGTGCCAAT

TCAATATGGT

GGATCTGGAC

CCCAGCCAAT

TCAATATGGC

GGACTTGGCA

7441

CCATGCCAAT

TCAATATGGC

GGACTTGGCA

CTGTGCCAAC

TGGGGAGGGG

TCTACTTGGC

7501

ACGGTGCCAA

GTTTGAGGAG

GGGTCTTGGC

CCTGTGCCAA

GTCCGCCATA

TTGAATTGGC

7561

ATGGTGCCAA

TAATGGCGGC

CATATTGGCT

ATATGCCAGG

ATCAATATAT

AGGCAATATC

7621

CAATATGGCC

CTATGCCAAT

ATGGCTATTG

GCCAGGTTCA

ATACTATGTA

TTGGCCCTAT

7681

GCCATATAGT

ATTCCATATA

TGGGTTTTCC

TATTGACGTA

GATAGCCCCT

CCCAATGGGC

7741

GGTCCCATAT

ACCATATATG

GGGCTTCCTA

ATACCGCCCA

TAGCCACTCC

CCCATTGACG

7801

TCAATGGTCT

CTATATATGG

TCTTTCCTAT

TGACGTCATA

TGGGCGGTCC

TATTGACGTA

7861

TATGGCGCCT

CCCCCATTGA

CGTCAATTAC

GGTAAATGGC

CCGCCTGGCT

CAATGCCCAT

7921

TGACGTCAAT

AGGACCACCC

ACCATTGACG

TCAATGGGAT

GGCTCATTGC

CCATTCATAT

7981

CCGTTCTCAC

GCCCCCTATT

GACGTCAATG

ACGGTAAATG

GCCCACTTGG

CAGTACATCA

8041

ATATCTATTA

ATAGTAACTT

GGCAAGTACA

TTACTATTGG

AAGGACGCCA

GGGTACATTG

8101

GCAGTACTCC

CATTGACGTC

AATGGCGGTA

AATGGCCCGC

GATGGCTGCC

AAGTACATCC

8161

CCATTGACGT

CAATGGGGAG

GGGCAATGAC

GCAAATGGGC

GTTCCATTGA

CGTAAATGGG

8221

CGGTAGGCGT

GCCTAATGGG

AGGTCTATAT

AAGCAATGCT

CGTTTAGGGA

AC

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