名称: | hCas9 |
---|---|
别称: | Plasmid#41815 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
反向引物: | HSVtk-pA-R:GTCTCCTTCCGTGTTTCAG |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATGGTGGTGT
CGAAGTACTT
GAAGGCTGCA
GGCGCGCCCA
AGTTGGTCAG
AGTAAACAAG
TGGATAATGT
TTTCTGCCTG
CTCCCTGATG
GGCTTATCCC
TGTGCTTATT
GTAAGCAGAA
AGCACCTTAT
CGAGGTTAGC
GTCGGCGAGG
ATCACTCTTT
TGGAGAATTC
GCTTATTTGC
TCGATGATCT
CATCAAGGTA
GTGTTTGTGT
TGTTCCACGA
ACAGCTGCTT
CTGCTCATTA
TCTTCGGGAG
ACCCTTTGAG
CTTTTCATAG
TGGCTGGCCA
GATACAAGAA
ATTAACGTAT
TTAGAGGGCA
GTGCCAGCTC
GTTACCTTTC
TGCAGCTCGC
CCGCACTAGC
GAGCATTCGT
TTCCGGCCGT
TTTCAAGCTC
AAAGAGAGAG
TACTTGGGAA
GCTTAATGAT
GAGGTCTTTT
TTGACCTCTT
TATATCCTTT
CGCCTCGAGA
AAGTCGATGG
GGTTTTTTTC
GAAGCTTGAT
CGCTCCATGA
TTGTGATGCC
CAGCAGTTCC
TTGACGCTTT
TGAGTTTTTT
AGACTTCCCT
TTCTCCACTT
TGGCCACAAC
CAGTACACTG
TAAGCGACTG
TAGGAGAATC
GAATCCGCCG
TATTTCTTGG
GGTCCCAATC
TTTTTTGCGT
GCGATCAGCT
TGTCGCTGTT
CCTTTTCGGG
AGGATACTTT
CCTTGGAGAA
GCCTCCGGTC
TGTACTTCGG
TCTTTTTAAC
GATGTTCACC
TGCGGCATGG
ACAGGACCTT
CCGGACTGTC
GCGAAATCCC
TACCCTTGTC
CCACACGATT
TCTCCTGTTT
CTCCGTTTGT
TTCGATAAGT
GGTCGCTTCC
GAATCTCTCC
ATTGGCCAGT
GTAATCTCGG
TCTTGAAAAA
ATTCATAATA
TTGCTGTAAA
AGAAGTACTT
AGCGGTGGCC
TTGCCTATTT
CCTGCTCAGA
CTTTGCGATC
ATTTTCCTAA
CATCGTACAC
TTTATAGTCT
CCGTAAACAA
ATTCAGATTC
AAGCTTGGGA
TATTTTTTGA
TAAGTGCAGT
GCCTACCACT
GCATTCAGGT
AGGCATCATG
CGCATGGTGG
TAATTGTTGA
TCTCTCTCAC
CTTATAAAAC
TGAAAGTCCT
TTCTGAAATC
TGAGACCAGC
TTAGACTTCA
GAGTAATAAC
TTTCACCTCT
CGAATCAGTT
TGTCATTTTC
ATCGTACTTG
GTGTTCATGC
GTGAATCGAG
AATTTGGGCC
ACGTGCTTGG
TGATCTGGCG
TGTCTCAACA
AGCTGCCTTT
TGATGAAGCC
GGCTTTATCC
AACTCAGACA
GGCCACCTCG
TTCAGCCTTA
GTCAGATTAT
CGAACTTCCG
TTGTGTGATC
AGTTTGGCGT
TCAGCAGCTG
CCGCCAATAA
TTTTTCATTT
TCTTGACAAC
TTCTTCTGAG
GGGACGTTAT
CACTCTTCCC
TCTATTTTTA
TCGGATCTTG
TCAACACTTT
ATTATCAATA
GAATCATCTT
TGAGAAAAGA
CTGGGGCACG
ATATGATCCA
CGTCGTAGTC
GGAGAGCCGA
TTGATGTCCA
GTTCCTGATC
CACGTACATG
TCCCTGCCGT
TCTGCAGGTA
GTACAGGTAG
AGCTTCTCAT
TCTGAAGCTG
GGTGTTTTCA
ACTGGGTGTT
CCTTAAGGAT
TTGGGACCCC
AGTTCTTTTA
TACCCTCTTC
AATCCTCTTC
ATCCTTTCCC
TACTGTTCTT
CTGTCCCTTC
TGGGTAGTTT
GGTTCTCTCG
GGCCATCTCG
ATAACGATAT
TCTCGGGCTT
ATGCCTTCCC
ATTACTTTGA
CGAGTTCATC
CACGACCTTA
ACGGTCTGCA
GTATTCCCTT
TTTGATAGCT
GGGCTACCTG
CAAGATTAGC
GATGTGCTCG
TGAAGACTGT
CCCCCTGGCC
AGAAACTTGT
GCTTTCTGGA
TGTCCTCCTT
AAAGGTGAGA
GAGTCATCAT
GGATCAACTG
CATGAAGTTC
CGGTTGGCAA
ATCCATCGGA
CTTAAGAAAA
TCCAGGATTG
TCTTTCCACT
CTGCTTGTCT
CGGATCCCAT
TGATCAGTTT
TCTTGACAGC
CGCCCCCATC
CTGTATATCG
GCGCCTCTTG
AGCTGTTTCA
TGACTTTGTC
GTCGAAGAGA
TGAGCGTAAG
TTTTCAAGCG
TTCTTCAATC
ATCTCCCTAT
CTTCAAACAA
CGTAAGGGTG
AGGACAATGT
CCTCAAGAAT
GTCCTCGTTC
TCCTCATTGT
CCAGGAAGTC
CTTGTCTTTA
ATGATTTTCA
GGAGATCGTG
ATACGTTCCC
AGGGATGCGT
TGAAGCGATC
CTCCACTCCG
CTGATTTCAA
CAGAGTCGAA
ACATTCAATC
TTTTTGAAAT
AGTCTTCTTT
GAGCTGTTTC
ACGGTAACTT
TCCGGTTCGT
CTTGAAGAGG
AGGTCCACGA
TAGCTTTCTT
CTGCTCTCCA
GACAGGAATG
CTGGCTTTCT
CATCCCTTCT
GTGACGTATT
TGACCTTGGT
GAGCTCGTTA
TAAACTGTGA
AGTACTCGTA
CAGCAGAGAG
TGTTTAGGAA
GCACCTTTTC
GTTAGGCAGA
TTTTTATCAA
AGTTAGTCAT
CCTTTCGATG
AAGGACTGGG
CAGAGGCCCC
CTTATCCACG
ACTTCCTCGA
AGTTCCAGGG
AGTGATGGTC
TCTTCTGATT
TGCGAGTCAT
CCACGCGAAT
CTGGAATTTC
CCCGGGCGAG
GGGGCCTACA
TAGTAGGGTA
TCCGAAATGT
GAGGATTTTC
TCAATCTTTT
CCCTGTTATC
TTTCAAAAAG
GGGTAGAAAT
CCTCTTGCCG
CCTGAGGATA
GCGTGCAGTT
CGCCCAGGTG
AATCTGGTGG
GGGATGCTTC
CATTGTCGAA
AGTGCGCTGT
TTGCGCAACA
GATCTTCTCT
GTTAAGCTTT
ACCAGCAGCT
CCTCGGTGCC
GTCCATTTTT
TCCAAGATGG
GCTTAATAAA
TTTGTAAAAT
TCCTCCTGGC
TTGCTCCGCC
GTCAATGTAT
CCGGCGTAGC
CATTTTTAGA
CTGATCGAAG
AAAATTTCCT
TGTACTTCTC
AGGCAGTTGC
TGTCTGACAA
GGGCCTTCAG
CAAAGTCAAG
TCTTGGTGGT
GCTCATCATA
GCGCTTGATC
ATACTAGCGC
TCAGCGGAGC
TTTGGTGATC
TCCGTGTTCA
CTCGCAGAAT
ATCACTCAGC
AGAATGGCGT
CTGACAGGTT
CTTTGCCGCC
AAAAAAAGGT
CTGCGTACTG
GTCGCCGATC
TGGGCCAGCA
GATTGTCGAG
ATCATCATCG
TAGGTGTCTT
TGCTCAGTTG
AAGCTTGGCA
TCTTCGGCCA
GGTCGAAGTT
AGATTTAAAG
TTGGGGGTCA
GCCCGAGTGA
CAGGGCGATA
AGATTACCAA
ACAGGCCGTT
CTTCTTCTCC
CCAGGGAGCT
GTGCGATGAG
GTTTTCGAGC
CGCCGGGATT
TGGACAGCCT
AGCGCTCAGG
ATTGCTTTGG
CGTCAACTCC
GGATGCGTTG
ATCGGGTTCT
CTTCGAAAAG
CTGATTGTAA
GTCTGAACCA
GTTGGATAAA
GAGTTTGTCG
ACATCGCTGT
TGTCTGGGTT
CAGGTCCCCC
TCGATGAGGA
AGTGTCCCCG
AAATTTGATC
ATATGCGCCA
GCGCGAGATA
GATCAACCGC
AAGTCAGCCT
TATCAGTACT
GTCTACAAGC
TTCTTCCTCA
GATGATATAT
GGTTGGGTAC
TTTTCATGGT
ACGCCACCTC
GTCCACGATA
TTGCCAAAGA
TTGGGTGGCG
CTCGTGCTTT
TTATCCTCCT
CCACCAAAAA
GGACTCCTCC
AGCCTATGGA
AGAAAGAGTC
ATCCACCTTA
GCCATCTCAT
TACTAAAGAT
CTCCTGCAGG
TAGCAGATCC
GATTCTTTCT
GCGGGTATAT
CTGCGCCGTG
CTGTTCTTTT
GAGCCGCGTG
GCTTCGGCCG
TCTCCCCGGA
GTCGAACAGG
AGGGCGCCAA
TGAGGTTCTT
CTTTATGCTG
TGGCGATCGG
TATTGCCCAG
AACTTTGAAT
TTTTTGCTCG
GCACCTTGTA
CTCGTCCGTA
ATGACGGCCC
AGCCGACGCT
GTTTGTGCCG
ATATCGAGCC
CAATGGAGTA
CTTCTTGTCC
ATGGTGGCAA
GGGTTCGATC
CTCTAGAGTC
CGGAGGCTGG
ATCGGTCCCG
GTGTCTTCTA
TGGAGGTCAA
AACAGCGTGG
ATGGCGTCTC
CAGGCGATCT
GACGGTTCAC
TAAACGAGCT
CTGCTTATAT
AGACCTCCCA
CCGTACACGC
CTACCGCCCA
TTTGCGTCAA
TGGGGCGGAG
TTGTTACGAC
ATTTTGGAAA
GTCCCGTTGA
TTTTGGTGCC
AAAACAAACT
CCCATTGACG
TCAATGGGGT
GGAGACTTGG
AAATCCCCGT
GAGTCAAACC
GCTATCCACG
CCCATTGATG
TACTGCCAAA
ACCGCATCAC
CATGGTAATA
GCGATGACTA
ATACGTAGAT
GTACTGCCAA
GTAGGAAAGT
CCCATAAGGT
CATGTACTGG
GCATAATGCC
AGGCGGGCCA
TTTACCGTCA
TTGACGTCAA
TAGGGGGCGT
ACTTGGCATA
TGATACACTT
GATGTACTGC
CAAGTGGGCA
GTTTACCGTA
AATACTCCAC
CCATTGACGT
CAATGGAAAG
TCCCTATTGG
CGTTACTATG
GGAACATACG
TCATTATTGA
CGTCAATGGG
CGGGGGTCGT
TGGGCGGTCA
GCCAGGCGGG
CCATTTACCG
TAAGTTATGT
AACGCGGAAC
TCCATATATG
GGCTATGAAC
TAATGACCCC
GTAATTGATT
ACTATTAATA
ACTAGTCAAT
AATCAATGTC
AACGCGTATA
TCTGGCCCGT
ACATCGCGAA
GCAGCGCAAA
ACGCCTAACC
CTAAGCAGAT
TCTTCATGCA
ATTGTCGGTC
AAGCCTTGCC
TTGTTGTAGC
TTAAATTTTG
CTCGCGCACT
ACTCAGCGAC
CTCCAACACA
CAAGCAGGGA
GCAGATACTG
GCTTAACTAT
GCGGCATCAG
AGCAGATTGT
ACTGAGAGTG
CACCATAGGG
GATCGGGAGA
TCTCCCGATC
CGTCGACGTC
AGGTGGCACT
TTTCGGGGAA
ATGTGCGCGG
AACCCCTATT
TGTTTATTTT
TCTAAATACA
TTCAAATATG
TATCCGCTCA
TGAGACAATA
ACCCTGATAA
ATGCTTCAAT
AATATTGAAA
AAGGAAGAGT
ATGAGTATTC
AACATTTCCG
TGTCGCCCTT
ATTCCCTTTT
TTGCGGCATT
TTGCCTTCCT
GTTTTTGCTC
ACCCAGAAAC
GCTGGTGAAA
GTAAAAGATG
CTGAAGATCA
GTTGGGTGCA
CGAGTGGGTT
ACATCGAACT
GGATCTCAAC
AGCGGTAAGA
TCCTTGAGAG
TTTTCGCCCC
GAAGAACGTT
TTCCAATGAT
GAGCACTTTT
AAAGTTCTGC
TATGTGGCGC
GGTATTATCC
CGTATTGACG
CCGGGCAAGA
GCAACTCGGT
CGCCGCATAC
ACTATTCTCA
GAATGACTTG
GTTGAGTACT
CACCAGTCAC
AGAAAAGCAT
CTTACGGATG
GCATGACAGT
AAGAGAATTA
TGCAGTGCTG
CCATAACCAT
GAGTGATAAC
ACTGCGGCCA
ACTTACTTCT
GACAACGATC
GGAGGACCGA
AGGAGCTAAC
CGCTTTTTTG
CACAACATGG
GGGATCATGT
AACTCGCCTT
GATCGTTGGG
AACCGGAGCT
GAATGAAGCC
ATACCAAACG
ACGAGCGTGA
CACCACGATG
CCTGTAGCAA
TGGCAACAAC
GTTGCGCAAA
CTATTAACTG
GCGAACTACT
TACTCTAGCT
TCCCGGCAAC
AATTAATAGA
CTGGATGGAG
GCGGATAAAG
TTGCAGGACC
ACTTCTGCGC
TCGGCCCTTC
CGGCTGGCTG
GTTTATTGCT
GATAAATCTG
GAGCCGGTGA
GCGTGGGTCT
CGCGGTATCA
TTGCAGCACT
GGGGCCAGAT
GGTAAGCCCT
CCCGTATCGT
AGTTATCTAC
ACGACGGGGA
GTCAGGCAAC
TATGGATGAA
CGAAATAGAC
AGATCGCTGA
GATAGGTGCC
TCACTGATTA
AGCATTGGTA
ACTGTCAGAC
CAAGTTTACT
CATATATACT
TTAGATTGAT
TTAAAACTTC
ATTTTTAATT
TAAAAGGATC
TAGGTGAAGA
TCCTTTTTGA
TAATCTCATG
ACCAAAATCC
CTTAACGTGA
GTTTTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTTC
TTCTAGTGTA
GCCGTAGTTA
GGCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCCAA
TACGCAAACC
GCCTCTCCCC
GCGCGTTGGC
CGATTCATTA
ATGCAGCTGG
CACGACAGGT
TTCCCGACTG
GAAAGCGGGC
AGTGAGCGCA
ACGCAATTAA
TGTGAGTTAG
CTCACTCATT
AGGCACCCCA
GGCTTTACAC
TTTATGCTTC
CGGCTCGTAT
GTTGTGTGGA
ATTGTGAGCG
GATAACAATT
TCACACAGGA
AACAGCTATG
ACCATGATTA
CGCCAAGCTC
TAGCTAGAGG
TCGACGGTAT
ACAGACATGA
TAAGATACAT
TGATGAGTTT
GGACAAACCA
CAACTAGAAT
GCAGTGAAAA
AAATGCTTTA
TTTGTGAAAT
TTGTGATGCT
ATTGCTTTAT
TTGTAACCAT
TATAAGCTGC
AATAAACAAG
TTGGGGTGGG
CGAAGAACTC
CAGCATGAGA
TCCCCGCGCT
GGAGGATCAT
CCAGCCGGCG
TCCCGGAAAA
CGATTCCGAA
GCCCAACCTT
TCATAGAAGG
CGGCGGTGGA
ATCGAAATCT
CGTGATGGCA
GGTTGGGCGT
CGCTTGGTCG
GTCATTTCGC
GAACCCCAGA
GTCCCGCTCA
GAAGAACTCG
TCAAGAAGGC
GATAGAAGGC
GATGCGCTGC
GAATCGGGAG
CGGCGATACC
GTAAAGCACG
AGGAAGCGGT
CAGCCCATTC
GCCGCCAAGC
TCTTCAGCAA
TATCACGGGT
AGCCAACGCT
ATGTCCTGAT
AGCGGTCCGC
CACACCCAGC
CGGCCACAGT
CGATGAATCC
AGAAAAGCGG
CCATTTTCCA
CCATGATATT
CGGCAAGCAG
GCATCGCCAT
GGGTCACGAC
GAGATCCTCG
CCGTCGGGCA
TGCGCGCCTT
GAGCCTGGCG
AACAGTTCGG
CTGGCGCGAG
CCCCTGATGC
TCTTCGTCCA
GATCATCCTG
ATCGACAAGA
CCGGCTTCCA
TCCGAGTACG
TGCTCGCTCG
ATGCGATGTT
TCGCTTGGTG
GTCGAATGGG
CAGGTAGCCG
GATCAAGCGT
ATGCAGCCGC
CGCATTGCAT
CAGCCATGAT
GGATACTTTC
TCGGCAGGAG
CAAGGTGAGA
TGACAGGAGA
TCCTGCCCCG
GCACTTCGCC
CAATAGCAGC
CAGTCCCTTC
CCGCTTCAGT
GACAACGTCG
AGCACAGCTG
CGCAAGGAAC
GCCCGTCGTG
GCCAGCCACG
ATAGCCGCGC
TGCCTCGTCC
TGCAGTTCAT
TCAGGGCACC
GGACAGGTCG
GTCTTGACAA
AAAGAACCGG
GCGCCCCTGC
GCTGACAGCC
GGAACACGGC
GGCATCAGAG
CAGCCGATTG
TCTGTTGTGC
CCAGTCATAG
CCGAATAGCC
TCTCCACCCA
AGCGGCCGGA
GAACCTGCGT
GCAATCCATC
TTGTTCAATC
ATGCGAAACG
ATCCTCATCC
TGTCTCTTGA
TCAGATCCGA
AAATGGATAT
ACAAGCTCCC
GGGAGCTTTT
TGCAAAAGCC
TAGGCCTCCA
AAAAAGCCTC
CTCACTACTT
CTGGAATAGC
TCAGAGGCAG
AGGCGGCCTC
GGCCTCTGCA
TAAATAAAAA
AAATTAGTCA
GCCATGGGGC
GGAGAATGGG
CGGAACTGGG
CGGAGTTAGG
GGCGGGATGG
GCGGAGTTAG
GGGCGGGACT
ATGGTTGCTG
ACTAATTGAG
ATGCATGCTT
TGCATACTTC
TGCCTGCTGG
GGAGCCTGGG
GACTTTCCAC
ACCTGGTTGC
TGACTAATTG
AGATGCATGC
TTTGCATACT
TCTGCCTGCT
GGGGAGCCTG
GGGACTTTCC
ACACCCTAAC
TGACACACAT
TCCACAGAAT
TAATTCGCGT
TAAATTTTTG
TTAAATCAGC
TCATTTTTTA
ACCAATAGGC
CGAAATCGGC
AAAATCCCTT
ATAAATCAAA
AGAATAGACC
GAGATAGGGT
TGAGTGTTGT
TCCAGTTTGG
AACAAGAGTC
CACTATTAAA
GAACGTGGAC
TCCAACGTCA
AAGGGCGAAA
AACCGTCTAT
CAGGGCGATG
GCCCACTACG
TGAACCATCA
CCCTAATCAA
GTTTTTTGGG
GTCGAGGTGC
CGTAAAGCAC
TAAATCGGAA
CCCTAAAGGG
AGCCCCCGAT
TTAGAGCTTG
ACGGGGAAAG
CCGGCGAACG
TGGCGAGAAA
GGAAGGGAAG
AAAGCGAAAG
GAGCGGGCGC
TAGGGCGCTG
GCAAGTGTAG
CGGTCACGCT
GCGCGTAACC
ACCACACCCG
CCGCGCTTAA
TGCGCCGCTA
CAGGGCGCGT
GGGGATACCC
CCTAGAGCCC
CAGCTGCGCA
GATCTGCTAT
GGCAGGGCCT
GCCGCCCCGA
CGTTGGCTGC
GAGCCCTGGG
CCTTCACCCG
AACTTGGGGG
GTGGGGTGGG
GAAAAGGAAG
AAACGCGGGC
GTATTGGCCC
CAATGGGGTC
TCGGTGGGGT
ATCGACAGAG
TGCCAGCCCT
GGGACCGAAC
CCCGCGTTTA
TGAACAAACG
ACCCAACACC
CGTGCGTTTT
ATTCTGTCTT
TTTATTGCCG
TCATAGCGCG
GGTTCCTTCC
GGTATTGTCT
CCTTCCGTGT
TTCAGTTAGC
CTCCCCCGTT
TAAACTCATT
ACTAACCGGT
AGGGATCGAA
CCCTTTCACA
CCTTCCTCTT
CTTCTTGGGG
TCAGCCCTGC
TGTCTCCACC
GAGCTGAGAG
AGGTCGATTC
TTGTTTCATA
GAGCCCCGTA
ATTGACTGAT
GAATCAGTGT
GGCGTCCAGG
ACCTCCTTTG
TAGAGGTGTA
CCGCTTTCTG
TCT