产品货号：P0741
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pX330A-FBL-PITCh |
|---|---|
| 别称: | Plasmid#63671;pX330A-FBL/PITCh |
| 基因长: | |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | 多框空载 |
| 原核抗性： | Amp |
| 筛选标记： | |
| 荧光标记： | |
| 启动子: | U6 |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pX330 |
| 正向引物: | Forward(left ITR)：CCTCTGACTTGAGCGTCGAT |
| 反向引物: | BGH：TAGAAGGCACAGTCGAGG |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
AGGTACCCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAGTA
ACGCCAATAG
GGACTTTCCA
TTGACGTCAA
TGGGTGGAGT
ATTTACGGTA
AACTGCCCAC
TTGGCAGTAC
ATCAAGTGTA
TCATATGCCA
AGTACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCG
CCTGGCATTG
TGCCCAGTAC
ATGACCTTAT
GGGACTTTCC
TACTTGGCAG
TACATCTACG
TATTAGTCAT
CGCTATTACC
ATGGTCGAGG
TGAGCCCCAC
GTTCTGCTTC
ACTCTCCCCA
TCTCCCCCCC
CTCCCCACCC
CCAATTTTGT
ATTTATTTAT
TTTTTAATTA
TTTTGTGCAG
CGATGGGGGC
GGGGGGGGGG
GGGGGGCGCG
CGCCAGGCGG
GGCGGGGCGG
GGCGAGGGGC
GGGGCGGGGC
GAGGCGGAGA
GGTGCGGCGG
CAGCCAATCA
GAGCGGCGCG
CTCCGAAAGT
TTCCTTTTAT
GGCGAGGCGG
CGGCGGCGGC
GGCCCTATAA
AAAGCGAAGC
GCGCGGCGGG
CGGGAGTCGC
TGCGACGCTG
CCTTCGCCCC
GTGCCCCGCT
CCGCCGCCGC
CTCGCGCCGC
CCGCCCCGGC
TCTGACTGAC
CGCGTTACTC
CCACAGGTGA
GCGGGCGGGA
CGGCCCTTCT
CCTCCGGGCT
GTAATTAGCT
GAGCAAGAGG
TAAGGGTTTA
AGGGATGGTT
GGTTGGTGGG
GTATTAATGT
TTAATTACCT
GGAGCACCTG
CCTGAAATCA
CTTTTTTTCA
GGTTGGACCG
GTGCCACCAT
GGACTATAAG
GACCACGACG
GAGACTACAA
GGATCATGAT
ATTGATTACA
AAGACGATGA
CGATAAGATG
GCCCCAAAGA
AGAAGCGGAA
GGTCGGTATC
CACGGAGTCC
CAGCAGCCGA
CAAGAAGTAC
AGCATCGGCC
TGGACATCGG
CACCAACTCT
GTGGGCTGGG
CCGTGATCAC
CGACGAGTAC
AAGGTGCCCA
GCAAGAAATT
CAAGGTGCTG
GGCAACACCG
ACCGGCACAG
CATCAAGAAG
AACCTGATCG
GAGCCCTGCT
GTTCGACAGC
GGCGAAACAG
CCGAGGCCAC
CCGGCTGAAG
AGAACCGCCA
GAAGAAGATA
CACCAGACGG
AAGAACCGGA
TCTGCTATCT
GCAAGAGATC
TTCAGCAACG
AGATGGCCAA
GGTGGACGAC
AGCTTCTTCC
ACAGACTGGA
AGAGTCCTTC
CTGGTGGAAG
AGGATAAGAA
GCACGAGCGG
CACCCCATCT
TCGGCAACAT
CGTGGACGAG
GTGGCCTACC
ACGAGAAGTA
CCCCACCATC
TACCACCTGA
GAAAGAAACT
GGTGGACAGC
ACCGACAAGG
CCGACCTGCG
GCTGATCTAT
CTGGCCCTGG
CCCACATGAT
CAAGTTCCGG
GGCCACTTCC
TGATCGAGGG
CGACCTGAAC
CCCGACAACA
GCGACGTGGA
CAAGCTGTTC
ATCCAGCTGG
TGCAGACCTA
CAACCAGCTG
TTCGAGGAAA
ACCCCATCAA
CGCCAGCGGC
GTGGACGCCA
AGGCCATCCT
GTCTGCCAGA
CTGAGCAAGA
GCAGACGGCT
GGAAAATCTG
ATCGCCCAGC
TGCCCGGCGA
GAAGAAGAAT
GGCCTGTTCG
GAAACCTGAT
TGCCCTGAGC
CTGGGCCTGA
CCCCCAACTT
CAAGAGCAAC
TTCGACCTGG
CCGAGGATGC
CAAACTGCAG
CTGAGCAAGG
ACACCTACGA
CGACGACCTG
GACAACCTGC
TGGCCCAGAT
CGGCGACCAG
TACGCCGACC
TGTTTCTGGC
CGCCAAGAAC
CTGTCCGACG
CCATCCTGCT
GAGCGACATC
CTGAGAGTGA
ACACCGAGAT
CACCAAGGCC
CCCCTGAGCG
CCTCTATGAT
CAAGAGATAC
GACGAGCACC
ACCAGGACCT
GACCCTGCTG
AAAGCTCTCG
TGCGGCAGCA
GCTGCCTGAG
AAGTACAAAG
AGATTTTCTT
CGACCAGAGC
AAGAACGGCT
ACGCCGGCTA
CATTGACGGC
GGAGCCAGCC
AGGAAGAGTT
CTACAAGTTC
ATCAAGCCCA
TCCTGGAAAA
GATGGACGGC
ACCGAGGAAC
TGCTCGTGAA
GCTGAACAGA
GAGGACCTGC
TGCGGAAGCA
GCGGACCTTC
GACAACGGCA
GCATCCCCCA
CCAGATCCAC
CTGGGAGAGC
TGCACGCCAT
TCTGCGGCGG
CAGGAAGATT
TTTACCCATT
CCTGAAGGAC
AACCGGGAAA
AGATCGAGAA
GATCCTGACC
TTCCGCATCC
CCTACTACGT
GGGCCCTCTG
GCCAGGGGAA
ACAGCAGATT
CGCCTGGATG
ACCAGAAAGA
GCGAGGAAAC
CATCACCCCC
TGGAACTTCG
AGGAAGTGGT
GGACAAGGGC
GCTTCCGCCC
AGAGCTTCAT
CGAGCGGATG
ACCAACTTCG
ATAAGAACCT
GCCCAACGAG
AAGGTGCTGC
CCAAGCACAG
CCTGCTGTAC
GAGTACTTCA
CCGTGTATAA
CGAGCTGACC
AAAGTGAAAT
ACGTGACCGA
GGGAATGAGA
AAGCCCGCCT
TCCTGAGCGG
CGAGCAGAAA
AAGGCCATCG
TGGACCTGCT
GTTCAAGACC
AACCGGAAAG
TGACCGTGAA
GCAGCTGAAA
GAGGACTACT
TCAAGAAAAT
CGAGTGCTTC
GACTCCGTGG
AAATCTCCGG
CGTGGAAGAT
CGGTTCAACG
CCTCCCTGGG
CACATACCAC
GATCTGCTGA
AAATTATCAA
GGACAAGGAC
TTCCTGGACA
ATGAGGAAAA
CGAGGACATT
CTGGAAGATA
TCGTGCTGAC
CCTGACACTG
TTTGAGGACA
GAGAGATGAT
CGAGGAACGG
CTGAAAACCT
ATGCCCACCT
GTTCGACGAC
AAAGTGATGA
AGCAGCTGAA
GCGGCGGAGA
TACACCGGCT
GGGGCAGGCT
GAGCCGGAAG
CTGATCAACG
GCATCCGGGA
CAAGCAGTCC
GGCAAGACAA
TCCTGGATTT
CCTGAAGTCC
GACGGCTTCG
CCAACAGAAA
CTTCATGCAG
CTGATCCACG
ACGACAGCCT
GACCTTTAAA
GAGGACATCC
AGAAAGCCCA
GGTGTCCGGC
CAGGGCGATA
GCCTGCACGA
GCACATTGCC
AATCTGGCCG
GCAGCCCCGC
CATTAAGAAG
GGCATCCTGC
AGACAGTGAA
GGTGGTGGAC
GAGCTCGTGA
AAGTGATGGG
CCGGCACAAG
CCCGAGAACA
TCGTGATCGA
AATGGCCAGA
GAGAACCAGA
CCACCCAGAA
GGGACAGAAG
AACAGCCGCG
AGAGAATGAA
GCGGATCGAA
GAGGGCATCA
AAGAGCTGGG
CAGCCAGATC
CTGAAAGAAC
ACCCCGTGGA
AAACACCCAG
CTGCAGAACG
AGAAGCTGTA
CCTGTACTAC
CTGCAGAATG
GGCGGGATAT
GTACGTGGAC
CAGGAACTGG
ACATCAACCG
GCTGTCCGAC
TACGATGTGG
ACCATATCGT
GCCTCAGAGC
TTTCTGAAGG
ACGACTCCAT
CGACAACAAG
GTGCTGACCA
GAAGCGACAA
GAACCGGGGC
AAGAGCGACA
ACGTGCCCTC
CGAAGAGGTC
GTGAAGAAGA
TGAAGAACTA
CTGGCGGCAG
CTGCTGAACG
CCAAGCTGAT
TACCCAGAGA
AAGTTCGACA
ATCTGACCAA
GGCCGAGAGA
GGCGGCCTGA
GCGAACTGGA
TAAGGCCGGC
TTCATCAAGA
GACAGCTGGT
GGAAACCCGG
CAGATCACAA
AGCACGTGGC
ACAGATCCTG
GACTCCCGGA
TGAACACTAA
GTACGACGAG
AATGACAAGC
TGATCCGGGA
AGTGAAAGTG
ATCACCCTGA
AGTCCAAGCT
GGTGTCCGAT
TTCCGGAAGG
ATTTCCAGTT
TTACAAAGTG
CGCGAGATCA
ACAACTACCA
CCACGCCCAC
GACGCCTACC
TGAACGCCGT
CGTGGGAACC
GCCCTGATCA
AAAAGTACCC
TAAGCTGGAA
AGCGAGTTCG
TGTACGGCGA
CTACAAGGTG
TACGACGTGC
GGAAGATGAT
CGCCAAGAGC
GAGCAGGAAA
TCGGCAAGGC
TACCGCCAAG
TACTTCTTCT
ACAGCAACAT
CATGAACTTT
TTCAAGACCG
AGATTACCCT
GGCCAACGGC
GAGATCCGGA
AGCGGCCTCT
GATCGAGACA
AACGGCGAAA
CCGGGGAGAT
CGTGTGGGAT
AAGGGCCGGG
ATTTTGCCAC
CGTGCGGAAA
GTGCTGAGCA
TGCCCCAAGT
GAATATCGTG
AAAAAGACCG
AGGTGCAGAC
AGGCGGCTTC
AGCAAAGAGT
CTATCCTGCC
CAAGAGGAAC
AGCGATAAGC
TGATCGCCAG
AAAGAAGGAC
TGGGACCCTA
AGAAGTACGG
CGGCTTCGAC
AGCCCCACCG
TGGCCTATTC
TGTGCTGGTG
GTGGCCAAAG
TGGAAAAGGG
CAAGTCCAAG
AAACTGAAGA
GTGTGAAAGA
GCTGCTGGGG
ATCACCATCA
TGGAAAGAAG
CAGCTTCGAG
AAGAATCCCA
TCGACTTTCT
GGAAGCCAAG
GGCTACAAAG
AAGTGAAAAA
GGACCTGATC
ATCAAGCTGC
CTAAGTACTC
CCTGTTCGAG
CTGGAAAACG
GCCGGAAGAG
AATGCTGGCC
TCTGCCGGCG
AACTGCAGAA
GGGAAACGAA
CTGGCCCTGC
CCTCCAAATA
TGTGAACTTC
CTGTACCTGG
CCAGCCACTA
TGAGAAGCTG
AAGGGCTCCC
CCGAGGATAA
TGAGCAGAAA
CAGCTGTTTG
TGGAACAGCA
CAAGCACTAC
CTGGACGAGA
TCATCGAGCA
GATCAGCGAG
TTCTCCAAGA
GAGTGATCCT
GGCCGACGCT
AATCTGGACA
AAGTGCTGTC
CGCCTACAAC
AAGCACCGGG
ATAAGCCCAT
CAGAGAGCAG
GCCGAGAATA
TCATCCACCT
GTTTACCCTG
ACCAATCTGG
GAGCCCCTGC
CGCCTTCAAG
TACTTTGACA
CCACCATCGA
CCGGAAGAGG
TACACCAGCA
CCAAAGAGGT
GCTGGACGCC
ACCCTGATCC
ACCAGAGCAT
CACCGGCCTG
TACGAGACAC
GGATCGACCT
GTCTCAGCTG
GGAGGCGACA
AAAGGCCGGC
GGCCACGAAA
AAGGCCGGCC
AGGCAAAAAA
GAAAAAGTAA
GAATTCCTAG
AGCTCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGTGGGGCA
GGACAGCAAG
GGGGAGGATT
GGGAAGAGAA
TAGCAGGCAT
GCTGGGGAGC
GGCCGCAGGA
ACCCCTAGTG
ATGGAGTTGG
CCACTCCCTC
TCTGCGCGCT
CGCTCGCTCA
CTGAGGCCGG
GCGACCAAAG
GTCGCCCGAC
GCCCGGGCTT
TGCCCGGGCG
GCCTCAGTGA
GCGAGCGAGC
GCGCAGCTGC
CTGCAGGGGC
GCCTGATGCG
GTATTTTCTC
CTTACGCATC
TGTGCGGTAT
TTCACACCGC
ATACGTCAAA
GCAACCATAG
TACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTTGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGGCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
TTAACAAAAT
ATTAACGTTT
ACAATTTTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGCCC
CGACACCCGC
CAACACCCGC
TGACGCGCCC
TGACGGGCTT
GTCTGCTCCC
GGCATCCGCT
TACAGACAAG
CTGTGACCGT
CTCCGGGAGC
TGCATGTGTC
AGAGGTTTTC
ACCGTCATCA
CCGAAACGCG
CGAGACGAAA
GGGCCTCGTG
ATACGCCTAT
TTTTATAGGT
TAATGTCATG
ATAATAATGG
TTTCTTAGAC
GTCAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGA
AGCCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTGAGGGC
CTATTTCCCA
TGATTCCTTC
ATATTTGCAT
ATACGATACA
AGGCTGTTAG
AGAGATAATT
GGAATTAATT
TGACTGTAAA
CACAAAGATA
TTAGTACAAA
ATACGTGACG
TAGAAAGTAA
TAATTTCTTG
GGTAGTTTGC
AGTTTTAAAA
TTATGTTTTA
AAATGGACTA
TCATATGCTT
ACCGTAACTT
GAAAGTATTT
CGATTTCTTG
GCTTTATATA
TCTTGTGGAA
AGGACGAAAC
ACCGCTCTCA
CAGGCCACCC
CCCAGTTTTA
GAGCTAGAAA
TAGCAAGTTA
AAATAAGGCT
AGTCCGTTAT
CAACTTGAAA
AAGTGGCACC
GAGTCGGTGC
TTTTTTGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
TTTAGCGCGT
GCGCCAATTC
TGCAGACAAA
TGGCTCTAGA
GGCATGTGAG
GGCCTATTTC
CCATGATTCC
TTCATATTTG
CATATACGAT
ACAAGGCTGT
TAGAGAGATA
ATTGGAATTA
ATTTGACTGT
AAACACAAAG
ATATTAGTAC
AAAATACGTG
ACGTAGAAAG
TAATAATTTC
TTGGGTAGTT
TGCAGTTTTA
AAATTATGTT
TTAAAATGGA
CTATCATATG
CTTACCGTAA
CTTGAAAGTA
TTTCGATTTC
TTGGCTTTAT
ATATCTTGTG
GAAAGGACGA
AACACCGCAT
CGTACGCGTA
CGTGTTGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
ATCAACTTGA
AAAAGTGGCA
CCGAGTCGGT
GCTTTTTTGT
TTTAGAGCTA
GAAATAGCAA
GTTAAAATAA
GGCTAGTCCG
TTTTTAGCGC
GTGCGCCAAT
TCTGCAGACA
AATGGCTCTA
G
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