名称: | pGL3-3.6kb human IFN-gamma luc |
---|---|
别称: | Plasmid#17599 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 启动子检测 |
片段类型: | Promoter |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | Fluc |
启动子: | IFNgamma |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
反向引物: | GLP2:CTTTATGTTTTTGGCGTCTTCC |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATGGAAGACG
CCAAAAACAT
AAAGAAAGGC
CCGGCGCCAT
TCTATCCGCT
GGAAGATGGA
ACCGCTGGAG
AGCAACTGCA
TAAGGCTATG
AAGAGATACG
CCCTGGTTCC
TGGAACAATT
GCTTTTACAG
ATGCACATAT
CGAGGTGGAC
ATCACTTACG
CTGAGTACTT
CGAAATGTCC
GTTCGGTTGG
CAGAAGCTAT
GAAACGATAT
GGGCTGAATA
CAAATCACAG
AATCGTCGTA
TGCAGTGAAA
ACTCTCTTCA
ATTCTTTATG
CCGGTGTTGG
GCGCGTTATT
TATCGGAGTT
GCAGTTGCGC
CCGCGAACGA
CATTTATAAT
GAACGTGAAT
TGCTCAACAG
TATGGGCATT
TCGCAGCCTA
CCGTGGTGTT
CGTTTCCAAA
AAGGGGTTGC
AAAAAATTTT
GAACGTGCAA
AAAAAGCTCC
CAATCATCCA
AAAAATTATT
ATCATGGATT
CTAAAACGGA
TTACCAGGGA
TTTCAGTCGA
TGTACACGTT
CGTCACATCT
CATCTACCTC
CCGGTTTTAA
TGAATACGAT
TTTGTGCCAG
AGTCCTTCGA
TAGGGACAAG
ACAATTGCAC
TGATCATGAA
CTCCTCTGGA
TCTACTGGTC
TGCCTAAAGG
TGTCGCTCTG
CCTCATAGAA
CTGCCTGCGT
GAGATTCTCG
CATGCCAGAG
ATCCTATTTT
TGGCAATCAA
ATCATTCCGG
ATACTGCGAT
TTTAAGTGTT
GTTCCATTCC
ATCACGGTTT
TGGAATGTTT
ACTACACTCG
GATATTTGAT
ATGTGGATTT
CGAGTCGTCT
TAATGTATAG
ATTTGAAGAA
GAGCTGTTTC
TGAGGAGCCT
TCAGGATTAC
AAGATTCAAA
GTGCGCTGCT
GGTGCCAACC
CTATTCTCCT
TCTTCGCCAA
AAGCACTCTG
ATTGACAAAT
ACGATTTATC
TAATTTACAC
GAAATTGCTT
CTGGTGGCGC
TCCCCTCTCT
AAGGAAGTCG
GGGAAGCGGT
TGCCAAGAGG
TTCCATCTGC
CAGGTATCAG
GCAAGGATAT
GGGCTCACTG
AGACTACATC
AGCTATTCTG
ATTACACCCG
AGGGGGATGA
TAAACCGGGC
GCGGTCGGTA
AAGTTGTTCC
ATTTTTTGAA
GCGAAGGTTG
TGGATCTGGA
TACCGGGAAA
ACGCTGGGCG
TTAATCAAAG
AGGCGAACTG
TGTGTGAGAG
GTCCTATGAT
TATGTCCGGT
TATGTAAACA
ATCCGGAAGC
GACCAACGCC
TTGATTGACA
AGGATGGATG
GCTACATTCT
GGAGACATAG
CTTACTGGGA
CGAAGACGAA
CACTTCTTCA
TCGTTGACCG
CCTGAAGTCT
CTGATTAAGT
ACAAAGGCTA
TCAGGTGGCT
CCCGCTGAAT
TGGAATCCAT
CTTGCTCCAA
CACCCCAACA
TCTTCGACGC
AGGTGTCGCA
GGTCTTCCCG
ACGATGACGC
CGGTGAACTT
CCCGCCGCCG
TTGTTGTTTT
GGAGCACGGA
AAGACGATGA
CGGAAAAAGA
GATCGTGGAT
TACGTCGCCA
GTCAAGTAAC
AACCGCGAAA
AAGTTGCGCG
GAGGAGTTGT
GTTTGTGGAC
GAAGTACCGA
AAGGTCTTAC
CGGAAAACTC
GACGCAAGAA
AAATCAGAGA
GATCCTCATA
AAGGCCAAGA
AGGGCGGAAA
GATCGCCGTG
TAATTCTAGA
GTCGGGGCGG
CCGGCCGCTT
CGAGCAGACA
TGATAAGATA
CATTGATGAG
TTTGGACAAA
CCACAACTAG
AATGCAGTGA
AAAAAATGCT
TTATTTGTGA
AATTTGTGAT
GCTATTGCTT
TATTTGTAAC
CATTATAAGC
TGCAATAAAC
AAGTTAACAA
CAACAATTGC
ATTCATTTTA
TGTTTCAGGT
TCAGGGGGAG
GTGTGGGAGG
TTTTTTAAAG
CAAGTAAAAC
CTCTACAAAT
GTGGTAAAAT
CGATAAGGAT
CCGTCGACCG
ATGCCCTTGA
GAGCCTTCAA
CCCAGTCAGC
TCCTTCCGGT
GGGCGCGGGG
CATGACTATC
GTCGCCGCAC
TTATGACTGT
CTTCTTTATC
ATGCAACTCG
TAGGACAGGT
GCCGGCAGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGACGCGC
CCTGTAGCGG
CGCATTAAGC
GCGGCGGGTG
TGGTGGTTAC
GCGCAGCGTG
ACCGCTACAC
TTGCCAGCGC
CCTAGCGCCC
GCTCCTTTCG
CTTTCTTCCC
TTCCTTTCTC
GCCACGTTCG
CCGGCTTTCC
CCGTCAAGCT
CTAAATCGGG
GGCTCCCTTT
AGGGTTCCGA
TTTAGTGCTT
TACGGCACCT
CGACCCCAAA
AAACTTGATT
AGGGTGATGG
TTCACGTAGT
GGGCCATCGC
CCTGATAGAC
GGTTTTTCGC
CCTTTGACGT
TGGAGTCCAC
GTTCTTTAAT
AGTGGACTCT
TGTTCCAAAC
TGGAACAACA
CTCAACCCTA
TCTCGGTCTA
TTCTTTTGAT
TTATAAGGGA
TTTTGCCGAT
TTCGGCCTAT
TGGTTAAAAA
ATGAGCTGAT
TTAACAAAAA
TTTAACGCGA
ATTTTAACAA
AATATTAACG
CTTACAATTT
GCCATTCGCC
ATTCAGGCTG
CGCAACTGTT
GGGAAGGGCG
ATCGGTGCGG
GCCTCTTCGC
TATTACGCCA
GCCCAAGCTA
CCATGATAAG
TAAGTAATAT
TAAGGTACGG
GAGGTACTTG
GAGCGGCCGC
AATAAAATAT
CTTTATTTTC
ATTACATCTG
TGTGTTGGTT
TTTTGTGTGA
ATCGATAGTA
CTAACATACG
CTCTCCATCA
AAACAAAACG
AAACAAAACA
AACTAGCAAA
ATAGGCTGTC
CCCAGTGCAA
GTGCAGGTGC
CAGAACATTT
CTCTATCGAT
AGGTACCGAG
CTCTTACGCG
TGTGGAAACA
AGTGCAAAAC
GAAAATGTGT
GTCCTTTGTT
TGAAGAGTTA
TTAGAAATTT
CAAGGAAGTG
ACAACAGAGC
ATTAAACCAA
GTGTGGGCTG
TTCTAAGTTC
AGGGTCCTGT
CAGACTGCAC
AGGTTGCATG
TCCATGAAGT
CAGGTTGGCC
GGGATGTCTA
GGCAAAAATT
TCAAAAATAT
ATTTCAGAAA
CAATTTCAAA
TTATACTTGC
AGTCTTACTA
TGTTTTAAGG
AATTAAAGTG
TGATTACTAT
CTCACAAATT
CTACAGAAGA
AAAAATCTCA
GAAACTAAAG
CCTGATAGGG
TGTAGTTATT
TGAATTCACA
TATCTAACGT
AGAGGTACAT
GCAATCATCT
CAATGGATAT
TGATAGATTG
GGGGATGGAT
TGACTAAGGG
TTCCATCCTC
TTTGGGAAGA
TCTTGGATTT
AGACTCAGTT
CTGCATTAGC
CAGTTGAATG
AATTTAAGCA
CATCACTTGT
CTTTCTGGGT
CTGGATTTAC
AAATCTAAAA
GGTAAGGGGT
TGACCATAGA
TAATCTATAA
TTCCATAATT
CTGTGCTTGG
CCATGCCATC
AAGAGTTATT
AGAGCTTGGA
TTTTAGCCGT
GTAATTACTC
TTTGTAAACA
GTTGCCACAC
TGTAAACATA
TTCTTAATAT
AATCTGTTAT
TTTGAATAGG
CATTCTCAAT
TTACAAGTGG
CTAGGCAGTA
TATCATTCCC
TCTTATGGAT
CCCACTATTC
TTAACTCCTC
AATCCTAGTA
CTACATTCCA
GTGGAGCAAT
CAAGGACCAG
GCAGAACTAA
TTTTTACTTA
TTTCCAGACA
GTTGGGAACA
TTCCTTCCAG
TGACTCAGAG
GTTGGGCATT
GGAAGGTATT
GAGAACCTGC
AAAGTGTCTC
AGGCTCTGAC
CCAAATCCCT
GTGCCACCTG
CAAGCACGTT
CTCTGGACGT
AATTTTTCTT
GAGCAGAGCA
ACAGTAGAGC
TTTGTATGCA
ACAATGTAAT
TTTTACATTC
TTCACTTGCT
TAACATGAGG
TTTGGTTCCA
CATAAATGTT
TTGATCTTAT
TTATTCTTTG
GGAAGATCGC
CCCTCAAAGA
GCTTTCTGGC
TTTTTTTTCC
CACATAAAGG
AATACACGAA
ATTGTCGCAG
TCAGCAAGTG
TTGGCTAAAG
AGACACAGCA
TCATGTTTCT
AACATTAATC
ATAAATATTA
TTTAATTAAA
AACTAGTTTC
TTACAGGTTA
GGCCTGATAA
CATCGGAATT
AGAATTTGAG
GGCAAATAAC
TCAAAAGAGA
GCCTTAGGCA
AGCATTCGAA
TTTACATTTA
TTCAGTGCTC
TTTATGAGCA
AATCTCCATG
CTAAAATATT
TTAGGGAAGA
GAAAGCCATC
ATAAAAGCAA
TGAAACAATT
TCTGATTTTC
AGAAACTCAA
TCTAACATGG
CAGAAGACAC
GCGAATAGCT
ATCTTCAATC
AAGATTGAGG
AAACAGAACT
CTTAGAAAAG
TATAAACCAA
GACTTGTATA
GAGAATCTAA
GATTAATTTT
AAGGAGGATA
ATTTTGGAAA
AACTCAGGGA
GATGGTAATT
TTTAAGCCGG
GCTTGGATGG
ATGGCTACTA
CTCTCAGGGG
CACAAATGAG
GGGAAAAAGA
ACTCAAGACC
AAAGAAACAG
CATGAGCAAA
GGTCCAGGGT
ACTTTTTTTT
TTTTTTTTTA
AAGAAATGAC
TAGGCCGGGT
GCGGTGGCTC
ACGCCTGTAA
TCCCAGCACT
TTGGGAGGCC
AAGGCGGGCG
GATCACGAGG
TCAGGAGATC
GAGACCATCC
TGATTAACAC
AGTGAAACCC
CGTCTGTACT
AAAAATACCA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAnAAA
AAAAAAAAAA
Annnnnnnnn
nnnnnnnnnn
nnnnnnnnnn
nnnAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAATTA
GCCGGGCGTG
GCGAGTGCCT
GTAGTCCCAG
CTACTCGGGA
GGCTGAGGCG
GGAGAATGGC
GTGAATCCGG
GAGGCAGAGC
TTGCAGTGAG
CCGAGATTGC
GCCACTGCAC
TCCAGCCTGG
GTGACAGAGC
AAGACTCCGT
CTCAAAAAAA
AAAAAAAAAG
AAATGACTAG
TCATCCAATG
TGCCAAAATA
ATAATAAACT
TTTATTAGTG
ATTACTATAT
GCCAGGAAAA
ATTCCTAGCA
CTTTATGAGG
ATTACCTGAT
TTAATTTTCA
ACTGAAGCAT
GGAAGAAGAT
ACTATTATCA
AGCCAGTTTT
ACAGGTAAGG
AGACTGAGTC
ATAGAAGATT
TAAGAAGTTA
ACTCACAATC
ATATAGCTAG
ATAGTAGAGG
AGTCAGGAAT
CAAGTTTGCC
CCATAACTGC
AATACTGTTA
TGTACACAGT
ACAGGTAGAA
ATGCAAAGTG
GGTTTGAACC
AAAGAGTGGA
GGGCTTTTTG
TGCCATCCCA
AAGTGTTGTA
CTTCATAAAT
AAATTACAAA
GGAGGAGAAA
GAATCCTATT
TTTTTTTGTA
TCTGAAAGAC
AAAGAAATAA
AAAGTTAAAA
AGATTCTCTG
TTAGTACTGA
TTATTTGGAA
CAATAAATTG
TTTAGAGCTA
TGCTGTTCAA
TATAGTAGCA
CCTAGCAGTA
TGTGCCCATT
AAGCGTTTGA
AATACGACTA
GACCAAATTG
AGATGCACCG
TAGGCTTAAA
ATATACACTG
TATTTCTTTC
CTTTTTTCTT
TTTTTCTTTT
TTTTTTTTGA
GACGGAATCT
TACTCTGTCA
CCTAGGCTGG
AGTGTAGTGG
CGCGATCTCG
GCTCACTGCA
ACCTCCACCT
TCTGGGTTCA
AGCCATTCTC
CTGCCTCAGC
CTCCCTAGTA
GCTGAGATTA
CAGGCATACA
CCACCATGCC
TGGCTAATTT
TTTTGTATTT
TTAGTAGAGA
TGGGGTTTCA
CCATATTGGC
TAGGCTGGTC
TCAAACTCCT
GACCTTGTGA
TCCACCCGCC
TCAACCTCCC
AAAGTGCTGG
GATTACAGGT
GTGAGCCACT
GCGTCTGGAA
CTCCCCCTGG
GAATATTCTC
TACACTGTAT
TTCAAGGATT
TAATATGACA
AAAAGAATGT
CAAATACCTT
ATTAACAATG
TAGTATATTG
ATGCATACTG
AAGTACTATT
TGGGATATAT
TGGTTTAAAT
ACAATATATT
TTAAAATTAT
ATTTACCTTT
TAAAAAAACT
TTTATTAATG
AGGCTACTAG
ATCATTTAAA
TTTACCTGTG
TGGCTTGTAT
TGTATTTCTA
CTGGGCAGTG
CTGATCTAGA
GCAATTTGAA
ACTTGTGGTA
GATATTTTAC
TAACCAACTC
TGATGAAGGA
CTTCCTCACC
AAATTGTTCT
TTTAACCGCA
TTCTTTCCTT
GCTTTCTGGT
CATTTGCAAG
AAAAATTTTA
AAAGGCTGCC
CCTTTGTAAA
GGTTTGAGAG
GCCCTAGAAT
TTCGTTTTTC
ACTTGTTCCC
AACCACAAGC
AAATGATCAA
TGTGCTTTGT
GAATGAAGAG
TCAACATTTT
ACCAGGGCGA
AGTGGGGAGG
TACAAAAAAA
TTTCCAGTCC
TTGAATGGTG
TGAAGTAAAA
GTGCCTTCAA
AGAATCCCAC
CAGAATGGCA
CAGGTGGGCA
TAATGGGTCT
GTCTCATCGT
CAAAGGACCC
AAGGAGTCTA
AAGGAAACTC
TAACTACAAC
ACCCAAATGC
CACAAAACCT
TAGTTATTAA
TACAAACTAT
CATCCCTGCC
TATCTGTCAC
CATCTCATCT
TAAAAAACTT
GTGAAAATAC
GTAATCCTCA
GGAGACTTCA
ATTAGGTATA
AATACCAGCA
GCCAGAGGAG
GTGCAGCACA
TTGTTCTGAT
CATCTGAAGA
TCAGCTATTA
GACGCGTGCT
AGCCCGGGCT
CGAGATCTGC
GATCTAAGTA
AGCTTGGCAT
TCCGGTACTG
TTGGTAAAGC
CACC