名称: | pUC18-TMV |
---|---|
别称: | |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因模板 |
片段类型: | cDNA |
片段物种: | 病毒 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | M13F:TGTAAAACGACGGCCAGT |
反向引物: | M13R:CAGGAAACAGCTATGACC |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TCGCGCGTTT
CGGTGATGAC
GGTGAAAACC
TCTGACACAT
GCAGCTCCCG
GAGACGGTCA
CAGCTTGTCT
GTAAGCGGAT
GCCGGGAGCA
GACAAGCCCG
TCAGGGCGCG
TCAGCGGGTG
TTGGCGGGTG
TCGGGGCTGG
CTTAACTATG
CGGCATCAGA
GCAGATTGTA
CTGAGAGTGC
ACCATATGCG
GTGTGAAATA
CCGCACAGAT
GCGTAAGGAG
AAAATACCGC
ATCAGGCGCC
ATTCGCCATT
CAGGCTGCGC
AACTGTTGGG
AAGGGCGATC
GGTGCGGGCC
TCTTCGCTAT
TACGCCAGCT
GGCGAAAGGG
GGATGTGCTG
CAAGGCGATT
AAGTTGGGTA
ACGCCAGGGT
TTTCCCAGTC
ACGACGTTGT
AAAACGACGG
CCAGTGCCAA
GCTTAATACG
ACTCACTATA
GTATTTTTAC
AACAATTACC
AACAACAACA
AACAACAAAC
AACATTACAA
TTACTATTTA
CAATTACAAT
GGCATACACA
CAGACAGCTA
CCACATCAGC
TTTGCTGGAC
ACTGTCCGAG
GAAACAACTC
CTTGGTCAAT
GATCTAGCAA
AGCGTCGTCT
TTACGACACA
GCGGTTGAAG
AGTTTAACGC
TCGTGACCGC
AGGCCCAAGG
TGAACTTTTC
AAAAGTAATA
AGCGAGGAGC
AGACGCTTAT
TGCTACCCGG
GCGTATCCAG
AATTCCAAAT
TACATTTTAT
AACACGCAAA
ATGCCGTGCA
TTCGCTTGCA
GGTGGATTGC
GATCTTTAGA
ACTGGAATAT
CTGATGATGC
AAATTCCCTA
CGGATCATTG
ACTTATGACA
TAGGCGGGAA
TTTTGCATCG
CATCTGTTCA
AGGGACGAGC
ATATGTACAC
TGCTGCATGC
CCAACCTGGA
CGTTCGAGAC
ATCATGCGGC
ACGAAGGCCA
GAAAGACAGT
ATTGAACTAT
ACCTTTCTAG
GCTAGAGAGA
GGGGGGAAAA
CAGTCCCCAA
CTTCCAAAAG
GAAGCATTTG
ACAGATACGC
AGAAATTCCT
GAAGACGCTG
TCTGTCACAA
TACTTTCCAG
ACAATGCGAC
ATCAGCCGAT
GCAGCAATCA
GGCAGAGTGT
ATGCCATTGC
GCTACACAGC
ATATATGACA
TACCAGCCGA
TGAGTTCGGG
GCGGCACTCT
TGAGGAAAAA
TGTCCATACG
TGCTATGCCG
CTTTCCACTT
CTCTGAGAAC
CTGCTTCTTG
AAGATTCATA
CGTCAATTTG
GACGAAATCA
ACGCGTGTTT
TTCGCGCGAT
GGAGACAAGT
TGACCTTTTC
TTTTGCATCA
GAGAGTACTC
TTAATTATTG
TCATAGTTAT
TCTAATATTC
TTAAGTATGT
GTGCAAAACT
TACTTCCCGG
CCTCTAATAG
AGAGGTTTAC
ATGAAGGAGT
TTTTAGTCAC
CAGAGTTAAT
ACCTGGTTTT
GTAAGTTTTC
TAGAATAGAT
ACTTTTCTTT
TGTACAAAGG
TGTGGCCCAT
AAAAGTGTAG
ATAGTGAGCA
GTTTTATACT
GCAATGGAAG
ACGCATGGCA
TTACAAAAAG
ACTCTTGCAA
TGTGCAACAG
CGAGAGAATC
CTCCTTGAGG
ATTCATCATC
AGTCAATTAC
TGGTTTCCCA
AAATGAGGGA
TATGGTCATC
GTACCATTAT
TCGACATTTC
TTTGGAGACT
AGTAAGAGGA
CGCGCAAGGA
AGTCTTAGTG
TCCAAGGATT
TCGTGTTTAC
AGTGCTTAAC
CACATTCGAA
CATACCAGGC
GAAAGCTCTT
ACATACGCAA
ATGTTTTGTC
CTTTGTCGAA
TCGATTCGAT
CGAGGGTAAT
CATTAACGGT
GTGACAGCGA
GGTCCGAATG
GGATGTGGAC
AAATCTTTGT
TACAATCCTT
GTCCATGACG
TTTTACCTGC
ATACTAAGCT
TGCCGTTCTA
AAGGATGACT
TACTGATTAG
CAAGTTTAGT
CTCGGTTCGA
AAACGGTGTG
CCAGCATGTG
TGGGATGAGA
TTTCGCTGGC
GTTTGGGAAC
GCATTTCCCT
CCGTGAAAGA
GAGGCTCTTG
AACAGGAAAC
TTATCAGAGT
GGCAGGCGAC
GCATTAGAGA
TCAGGGTGCC
TGATCTATAT
GTGACCTTCC
ACGACAGATT
AGTGACTGAG
TACAAGGCCT
CTGTGGACAT
GCCTGCGCTT
GACATTAGGA
AGAAGATGGA
AGAAACGGAA
GTGATGTACA
ATGCACTTTC
AGAGTTATCG
GTGTTAAGGG
AGTCTGACAA
ATTCGATGTT
GATGTTTTTT
CCCAGATGTG
CCAATCTTTG
GAAGTTGACC
CAATGACGGC
AGCGAAGGTT
ATAGTCGCGG
TCATGAGCAA
TGAGAGCGGT
CTGACTCTCA
CATTTGAACG
ACCTACTGAG
GCGAATGTTG
CGCTAGCTTT
ACAGGATCAA
GAGAAGGCTT
CAGAAGGTGC
TTTGGTAGTT
ACCTCAAGAG
AAGTTGAAGA
ACCGTCCATG
AAGGGTTCGA
TGGCCAGAGG
AGAGTTACAA
TTAGCTGGTC
TTGCTGGAGA
TCATCCGGAG
TCGTCCTATT
CTAAGAACGA
GGAGATAGAG
TCTTTAGAGC
AGTTTCATAT
GGCAACGGCA
GATTCGTTAA
TTCGTAAGCA
GATGAGCTCG
ATTGTGTACA
CGGGTCCGAT
TAAAGTTCAG
CAAATGAAAA
ACTTTATCGA
TAGCCTGGTA
GCATCACTAT
CTGCTGCGGT
GTCGAATCTC
GTCAAGATCC
TCAAAGATAC
AGCTGCTATT
GACCTTGAAA
CCCGTCAAAA
GTTTGGAGTC
TTGGATGTTG
CATCTAGGAA
GTGGTTAATC
AAACCAACGG
CCAAGAGTCA
TGCATGGGGT
GTTGTTGAAA
CCCACGCGAG
GAAGTATCAT
GTGGCGCTTT
TGGAATATGA
TGAGCAGGGT
GTGGTGACAT
GCGATGATTG
GAGAAGAGTA
GCTGTCAGCT
CTGAGTCTGT
TGTTTATTCC
GACATGGCGA
AACTCAGAAC
TCTGCGCAGA
CTGCTTCGAA
ACGGAGAACC
GCATGTCAGT
AGCGCAAAGG
TTGTTCTTGT
GGACGGAGTT
CCGGGCTGTG
GGAAAACCAA
AGAAATTCTT
TCCAGGGTTA
ATTTTGATGA
AGATCTAATT
TTAGTACCTG
GGAAGCAAGC
CGCGGAAATG
ATCAGAAGAC
GTGCGAATTC
CTCAGGGATT
ATTGTGGCCA
CGAAGGACAA
CGTTAAAACC
GTTGATTCTT
TCATGATGAA
TTTTGGGAAA
AGCACACGCT
GTCAGTTCAA
GAGGTTATTC
ATTGATGAAG
GGTTGATGTT
GCATACTGGT
TGTGTTAATT
TTCTTGTGGC
GATGTCATTG
TGCGAAATTG
CATATGTTTA
CGGAGACACA
CAGCAGATTC
CATACATCAA
TAGAGTTTCA
GGATTCCCGT
ACCCCGCCCA
TTTTGCCAAA
TTGGAAGTTG
ACGAGGTGGA
GACACGCAGA
ACTACTCTCC
GTTGTCCAGC
CGATGTCACA
CATTATCTGA
ACAGGAGATA
TGAGGGCTTT
GTCATGAGCA
CTTCTTCGGT
TAAAAAGTCT
GTTTCGCAGG
AGATGGTCGG
CGGAGCCGCC
GTGATCAATC
CGATCTCAAA
ACCCTTGCAT
GGCAAGATCC
TGACTTTTAC
CCAATCGGAT
AAAGAAGCTC
TGCTTTCAAG
AGGGTATTCA
GATGTTCACA
CTGTGCATGA
AGTGCAAGGC
GAGACATACT
CTGATGTTTC
ACTAGTTAGG
TTAACCCCTA
CACCAGTCTC
CATCATTGCA
GGAGACAGCC
CACATGTTTT
GGTCGCATTG
TCAAGGCACA
CCTGTTCGCT
CAAGTACTAC
ACTGTTGTTA
TGGATCCTTT
AGTTAGTATC
ATTAGAGATC
TAGAGAAACT
TAGCTCGTAC
TTGTTAGATA
TGTATAAGGT
CGATGCAGGA
ACACAATAGC
AATTACAGAT
TGACTCGGTG
TTCAAAGGTT
CCAATCTTTT
TGTTGCAGCG
CCAAAGACTG
GTGATATTTC
TGATATGCAG
TTTTACTATG
ATAAGTGTCT
CCCAGGCAAC
AGCACCATGA
TGAATAATTT
TGATGCTGTT
ACCATGAGGT
TGACTGACAT
TTCATTGAAT
GTCAAAGATT
GCATATTGGA
TATGTCTAAG
TCTGTTGCTG
CGCCTAAGGA
TCAAATCAAA
CCACTAATAC
CTATGGTACG
AACGGCGGCA
GAAATGCCAC
GCCAGACTGG
ACTATTGGAA
AATTTAGTGG
CGATGATTAA
AAGGAACTTT
AACGCACCCG
AGTTGTCTGG
CATCATTGAT
ATTGAAAATA
CTGCATCTTT
AGTTGTAGAT
AAGTTTTTTG
ATAGTTATTT
GCTTAAAGAA
AAAAGAAAAC
CAAATAAAAA
TGTTTCTTTG
TTCAGTAGAG
AGTCTCTCAA
TAGATGGTTA
GAAAAGCAGG
AACAGGTAAC
AATAGGCCAG
CTCGCAGATT
TTGATTTTGT
AGATTTGCCA
GCAGTTGATC
AGTACAGACA
CATGATTAAA
GCACAACCCA
AGCAAAAATT
GGACACTTCA
ATCCAAACGG
AGTACCCGGC
TTTGCAGACG
ATTGTGTACC
ATTCAAAAAA
GATCAATGCA
ATATTTGGCC
CGTTGTTTAG
TGAGCTTACT
AGGCAATTAC
TGGACAGTGT
TGATTCGAGC
AGATTTTTGT
TTTTCACAAG
AAAGACACCA
GCGCAGATTG
AGGATTTCTT
CGGAGATCTC
GACAGTCATG
TGCCGATGGA
TGTCTTGGAG
CTGGATATAT
CAAAATACGA
CAAATCTCAG
AATGAATTCC
ACTGTGCAGT
AGAATACGAG
ATCTGGCGAA
GATTGGGTTT
TGAAGACTTC
TTGGGAGAAG
TTTGGAAACA
AGGGCATAGA
AAGACCACCC
TCAAGGATTA
TACCGCAGGT
ATAAAAACTT
GCATCTGGTA
TCAAAGAAAG
AGCGGGGACG
TCACGACGTT
CATTGGAAAC
ACTGTGATCA
TTGCTGCATG
TTTGGCCTCG
ATGCTTCCGA
TGGAGAAAAT
AATCAAAGGA
GCCTTTTGCG
GTGACGATAG
TCTGCTGTAC
TTTCCAAAGG
GTTGTGAGTT
TCCGGATGTG
CAACACTCCG
CGAATCTTAT
GTGGAATTTT
GAAGCAAAAC
TGTTTAAAAA
ACAGTATGGA
TACTTTTGCG
GAAGATATGT
AATACATCAC
GACAGAGGAT
GCATTGTGTA
TTACGATCCC
CTAAAGTTGA
TCTCGAAACT
TGGTGCTAAA
CACATCAAGG
ATTGGGAACA
CTTGGAGGAG
TTCAGAAGGT
CTCTTTGTGA
TGTTGCTGTT
TCGTTGAACA
ATTGTGCGTA
TTACACACAG
TTGGACGACG
CTGTATGGGA
GGTTCATAAG
ACCGCCCCTC
CAGGTTCGTT
TGTTTATAAA
AGTCTGGTGA
AGTATTTGTC
TGATAAAGTT
CTTTTTAGAA
GTTTGTTTAT
AGATGGCTCT
AGTTGTTAAA
GGAAAAGTGA
ATATCAATGA
GTTTATCGAC
CTGACAAAAA
TGGAGAAGAT
CTTACCGTCG
ATGTTTACCC
CTGTAAAGAG
TGTTATGTGT
TCCAAAGTTG
ATAAAATAAT
GGTTCATGAG
AATGAGTCAT
TGTCAGAGGT
GAACCTTCTT
AAAGGAGTTA
AGCTTATTGA
TAGTGGATAC
GTCTGTTTAG
CCGGTTTGGT
CGTCACGGGC
GAGTGGAACT
TGCCTGACAA
TTGCAGAGGA
GGTGTGAGCG
TGTGTCTGGT
GGACAAAAGG
ATGGAAAGAG
CCGACGAGGC
CACTCTCGGA
TCTTACTACA
CAGCAGCTGC
AAAGAAAAGA
TTTCAGTTCA
AGGTCGTTCC
CAATTATGCT
ATAACCACCC
AGGACGCGAT
GAAAAACGTC
TGGCAAGTTT
TAGTTAATAT
TAGAAATGTG
AAGATGTCAG
CGGGTTTCTG
TCCGCTTTCT
CTGGAGTTTG
TGTCGGTGTG
TATTGTTTAT
AGAAATAATA
TAAAATTAGG
TTTGAGAGAG
AAGATTACAA
ACGTGAGAGA
CGGAGGGCCC
ATGGAACTTA
CAGAAGAAGT
CGTTGATGAG
TTCATGGAAG
ATGTCCCTAT
GTCGATCAGG
CTTGCAAAGT
TTCGATCTCG
AACCGGAAAA
AAGAGTGATG
TCCGCAAAGG
GAAAAATAGT
AGTAATGATC
GGTCAGTGCC
GAACAAGAAC
TATAGAAATG
TTAAGGATTT
TGGAGGAATG
AGTTTTAAAA
AGAATAATTT
AATCGATGAT
GATTCGGAGG
CTACTGTCGC
CGAATCGGAT
TCGTTTTAAA
TATGTCTTAC
AGTATCACTA
CTCCATCTCA
GTTCGTGTTC
TTGTCATCAG
CGTGGGCCGA
CCCAATAGAG
TTAATTAATT
TATGTACTAA
TGCCTTAGGA
AATCAGTTTC
AAACACAACA
AGCTCGAACT
GTCGTTCAAA
GACAATTCAG
TGAGGTGTGG
AAACCTTCAC
CACAAGTAAC
TGTTAGGTTC
CCTGACAGTG
ACTTTAAGGT
GTACAGGTAC
AATGCGGTAT
TAGACCCGCT
AGTCACAGCA
CTGTTAGGTG
CATTCGACAC
TAGAAATAGA
ATAATAGAAG
TTGAAAATCA
GGCGAACCCC
ACGACTGCCG
AAACGTTAGA
TGCTACTCGT
AGAGTAGACG
ACGCAACGGT
GGCCATAAGG
AGCGCGATAA
ATAATTTAAT
AGTAGAATTG
ATCAGAGGAA
CCGGATCTTA
TAATCGGAGC
TCTTTCGAGA
GCTCTTCTGG
TTTGGTTTGG
ACCTCTGGTC
CTGCAACTTG
AGGTAGTCAA
GATGCATAAT
AAATAACGGA
TTGTGTCCGT
AATCACACGT
GGTGCGTACG
ATAACGCATA
GTGTTTTTCC
CTCCACTTAA
ATCGAAGGGT
TGTGTCTTGG
ATCGCGCGGG
TCAAATGTAT
ATGGTTCATA
TACATCCGCA
GGCACGTAAT
AAAGCGAGGG
GTTCGAATCC
CCCCGTTACC
CCCGGTAGGG
GCCCAGGTAC
CGAGCTCGAA
TTCGTAATCA
TGGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGACGTC
TAAGAAACCA
TTATTATCAT
GACATTAACC
TATAAAAATA
GGCGTATCAC
GAGGCCCTTT
CGTC