名称: | pShuttle-CMV-HMGB1 |
---|---|
别称: | HMG1;HMG3;SBP-1 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F: |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CATCATCAAT
AATATACCTT
ATTTTGGATT
GAAGCCAATA
TGATAATGAG
GGGGTGGAGT
TTGTGACGTG
GCGCGGGGCG
TGGGAACGGG
GCGGGTGACG
TAGTAGTGTG
GCGGAAGTGT
GATGTTGCAA
GTGTGGCGGA
ACACATGTAA
GCGACGGATG
TGGCAAAAGT
GACGTTTTTG
GTGTGCGCCG
GTGTACACAG
GAAGTGACAA
TTTTCGCGCG
GTTTTAGGCG
GATGTTGTAG
TAAATTTGGG
CGTAACCGAG
TAAGATTTGG
CCATTTTCGC
GGGAAAACTG
AATAAGAGGA
AGTGAAATCT
GAATAATTTT
GTGTTACTCA
TAGCGCGTAA
TACTGTAATA
GTAATCAATT
ACGGGGTCAT
TAGTTCATAG
CCCATATATG
GAGTTCCGCG
TTACATAACT
TACGGTAAAT
GGCCCGCCTG
GCTGACCGCC
CAACGACCCC
CGCCCATTGA
CGTCAATAAT
GACGTATGTT
CCCATAGTAA
CGCCAATAGG
GACTTTCCAT
TGACGTCAAT
GGGTGGAGTA
TTTACGGTAA
ACTGCCCACT
TGGCAGTACA
TCAAGTGTAT
CATATGCCAA
GTACGCCCCC
TATTGACGTC
AATGACGGTA
AATGGCCCGC
CTGGCATTAT
GCCCAGTACA
TGACCTTATG
GGACTTTCCT
ACTTGGCAGT
ACATCTACGT
ATTAGTCATC
GCTATTACCA
TGGTGATGCG
GTTTTGGCAG
TACATCAATG
GGCGTGGATA
GCGGTTTGAC
TCACGGGGAT
TTCCAAGTCT
CCACCCCATT
GACGTCAATG
GGAGTTTGTT
TTGGCACCAA
AATCAACGGG
ACTTTCCAAA
ATGTCGTAAC
AACTCCGCCC
CATTGACGCA
AATGGGCGGT
AGGCGTGTAC
GGTGGGAGGT
CTATATAAGC
AGAGCTGGTT
TAGTGAACCG
TCAGATCCGC
TAGAGATCTG
GTACCGCCAC
CATGGGCAAA
GGAGATCCTA
AGAAGCCGAG
AGGCAAAATG
TCATCATATG
CATTTTTTGT
GCAAACTTGT
CGGGAGGAGC
ATAAGAAGAA
GCACCCAGAT
GCTTCAGTCA
ACTTCTCAGA
GTTTTCTAAG
AAGTGCTCAG
AGAGGTGGAA
GACCATGTCT
GCTAAAGAGA
AAGGAAAATT
TGAAGATATG
GCAAAAGCGG
ACAAGGCCCG
TTATGAAAGA
GAAATGAAAA
CCTATATCCC
TCCCAAAGGG
GAGACAAAAA
AGAAGTTCAA
GGATCCCAAT
GCACCCAAGA
GGCCTCCTTC
GGCCTTCTTC
CTCTTCTGCT
CTGAGTATCG
CCCAAAAATC
AAAGGAGAAC
ATCCTGGCCT
GTCCATTGGT
GATGTTGCGA
AGAAACTGGG
AGAGATGTGG
AATAACACTG
CTGCAGATGA
CAAGCAGCCT
TATGAAAAGA
AGGCTGCGAA
GCTGAAGGAA
AAATACGAAA
AGGATATTGC
TGCATATCGA
GCTAAAGGAA
AGCCTGATGC
AGCAAAAAAG
GGAGTTGTCA
AGGCTGAAAA
AAGCAAGAAA
AAGAAGGAAG
AGGAGGAAGA
TGAGGAAGAT
GAAGAGGATG
AGGAGGAGGA
GGAAGATGAA
GAAGATGAAG
ATGAAGAAGA
AGATGATGAT
GATGAAGGCG
GCCGCGGAAG
CGGAGTGAAA
CAGACTTTGA
ATTTTGACCT
TCTCAAGTTG
GCGGGAGACG
TGGAGTCCAA
CCCTGGACCT
GTCGACGGAA
GCGGAACGCG
TGGAAGCGGA
GAGGGCAGAG
GAAGTCTGCT
AACATGCGGT
GACGTCGAGG
AGAATCCTGG
ACCTCTCGAG
GCCACCATGG
TGAGCAAGGG
CGAGGAGCTG
TTCACCGGGG
TGGTGCCCAT
CCTGGTCGAG
CTGGACGGCG
ACGTAAACGG
CCACAAGTTC
AGCGTGTCCG
GCGAGGGCGA
GGGCGATGCC
ACCTACGGCA
AGCTGACCCT
GAAGCTGATC
TGCACCACCG
GCAAGCTGCC
CGTGCCCTGG
CCCACCCTCG
TGACCACCCT
GGGCTACGGC
CTGCAGTGCT
TCGCCCGCTA
CCCCGACCAC
ATGAAGCAGC
ACGACTTCTT
CAAGTCCGCC
ATGCCCGAAG
GCTACGTCCA
GGAGCGCACC
ATCTTCTTCA
AGGACGACGG
CAACTACAAG
ACCCGCGCCG
AGGTGAAGTT
CGAGGGCGAC
ACCCTGGTGA
ACCGCATCGA
GCTGAAGGGC
ATCGACTTCA
AGGAGGACGG
CAACATCCTG
GGGCACAAGC
TGGAGTACAA
CTACAACAGC
CACAACGTCT
ATATCACCGC
CGACAAGCAG
AAGAACGGCA
TCAAGGCCAA
CTTCAAGATC
CGCCACAACA
TCGAGGACGG
CGGCGTGCAG
CTCGCCGACC
ACTACCAGCA
GAACACCCCC
ATCGGCGACG
GCCCCGTGCT
GCTGCCCGAC
AACCACTACC
TGAGCTACCA
GTCCGCCCTG
AGCAAAGACC
CCAACGAGAA
GCGCGATCAC
ATGGTCCTGC
TGGAGTTCGT
GACCGCCGCC
GGGATCACTC
TCGGCATGGA
CGAGCTGTAC
AAGTAGTAAT
GAGCAAGCTT
CTAGATAAGA
TATCCGATCC
ACCGGATCTA
GATAACTGAT
CATAATCAGC
CATACCACAT
TTGTAGAGGT
TTTACTTGCT
TTAAAAAACC
TCCCACACCT
CCCCCTGAAC
CTGAAACATA
AAATGAATGC
AATTGTTGTT
GTTAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTAACGCG
GATCTGGGCG
TGGTTAAGGG
TGGGAAAGAA
TATATAAGGT
GGGGGTCTTA
TGTAGTTTTG
TATCTGTTTT
GCAGCAGCCG
CCGCCGCCAT
GAGCACCAAC
TCGTTTGATG
GAAGCATTGT
GAGCTCATAT
TTGACAACGC
GCATGCCCCC
ATGGGCCGGG
GTGCGTCAGA
ATGTGATGGG
CTCCAGCATT
GATGGTCGCC
CCGTCCTGCC
CGCAAACTCT
ACTACCTTGA
CCTACGAGAC
CGTGTCTGGA
ACGCCGTTGG
AGACTGCAGC
CTCCGCCGCC
GCTTCAGCCG
CTGCAGCCAC
CGCCCGCGGG
ATTGTGACTG
ACTTTGCTTT
CCTGAGCCCG
CTTGCAAGCA
GTGCAGCTTC
CCGTTCATCC
GCCCGCGATG
ACAAGTTGAC
GGCTCTTTTG
GCACAATTGG
ATTCTTTGAC
CCGGGAACTT
AATGTCGTTT
CTCAGCAGCT
GTTGGATCTG
CGCCAGCAGG
TTTCTGCCCT
GAAGGCTTCC
TCCCCTCCCA
ATGCGGTTTA
AAACATAAAT
AAAAAACCAG
ACTCTGTTTG
GATTTGGATC
AAGCAAGTGT
CTTGCTGTCT
TTATTTAGGG
GTTTTGCGCG
CGCGGTAGGC
CCGGGACCAG
CGGTCTCGGT
CGTTGAGGGT
CCTGTGTATT
TTTTCCAGGA
CGTGGTAAAG
GTGACTCTGG
ATGTTCAGAT
ACATGGGCAT
AAGCCCGTCT
CTGGGGTGGA
GGTAGCACCA
CTGCAGAGCT
TCATGCTGCG
GGGTGGTGTT
GTAGATGATC
CAGTCGTAGC
AGGAGCGCTG
GGCGTGGTGC
CTAAAAATGT
CTTTCAGTAG
CAAGCTGATT
GCCAGGGGCA
GGCCCTTGGT
GTAAGTGTTT
ACAAAGCGGT
TAAGCTGGGA
TGGGTGCATA
CGTGGGGATA
TGAGATGCAT
CTTGGACTGT
ATTTTTAGGT
TGGCTATGTT
CCCAGCCATA
TCCCTCCGGG
GATTCATGTT
GTGCAGAACC
ACCAGCACAG
TGTATCCGGT
GCACTTGGGA
AATTTGTCAT
GTAGCTTAGA
AGGAAATGCG
TGGAAGAACT
TGGAGACGCC
CTTGTGACCT
CCAAGATTTT
CCATGCATTC
GTCCATAATG
ATGGCAATGG
GCCCACGGGC
GGCGGCCTGG
GCGAAGATAT
TTCTGGGATC
ACTAACGTCA
TAGTTGTGTT
CCAGGATGAG
ATCGTCATAG
GCCATTTTTA
CAAAGCGCGG
GCGGAGGGTG
CCAGACTGCG
GTATAATGGT
TCCATCCGGC
CCAGGGGCGT
AGTTACCCTC
ACAGATTTGC
ATTTCCCACG
CTTTGAGTTC
AGATGGGGGG
ATCATGTCTA
CCTGCGGGGC
GATGAAGAAA
ACGGTTTCCG
GGGTAGGGGA
GATCAGCTGG
GAAGAAAGCA
GGTTCCTGAG
CAGCTGCGAC
TTACCGCAGC
CGGTGGGCCC
GTAAATCACA
CCTATTACCG
GCTGCAACTG
GTAGTTAAGA
GAGCTGCAGC
TGCCGTCATC
CCTGAGCAGG
GGGGCCACTT
CGTTAAGCAT
GTCCCTGACT
CGCATGTTTT
CCCTGACCAA
ATCCGCCAGA
AGGCGCTCGC
CGCCCAGCGA
TAGCAGTTCT
TGCAAGGAAG
CAAAGTTTTT
CAACGGTTTG
AGACCGTCCG
CCGTAGGCAT
GCTTTTGAGC
GTTTGACCAA
GCAGTTCCAG
GCGGTCCCAC
AGCTCGGTCA
CCTGCTCTAC
GGCATCTCGA
TCCAGCATAT
CTCCTCGTTT
CGCGGGTTGG
GGCGGCTTTC
GCTGTACGGC
AGTAGTCGGT
GCTCGTCCAG
ACGGGCCAGG
GTCATGTCTT
TCCACGGGCG
CAGGGTCCTC
GTCAGCGTAG
TCTGGGTCAC
GGTGAAGGGG
TGCGCTCCGG
GCTGCGCGCT
GGCCAGGGTG
CGCTTGAGGC
TGGTCCTGCT
GGTGCTGAAG
CGCTGCCGGT
CTTCGCCCTG
CGCGTCGGCC
AGGTAGCATT
TGACCATGGT
GTCATAGTCC
AGCCCCTCCG
CGGCGTGGCC
CTTGGCGCGC
AGCTTGCCCT
TGGAGGAGGC
GCCGCACGAG
GGGCAGTGCA
GACTTTTGAG
GGCGTAGAGC
TTGGGCGCGA
GAAATACCGA
TTCCGGGGAG
TAGGCATCCG
CGCCGCAGGC
CCCGCAGACG
GTCTCGCATT
CCACGAGCCA
GGTGAGCTCT
GGCCGTTCGG
GGTCAAAAAC
CAGGTTTCCC
CCATGCTTTT
TGATGCGTTT
CTTACCTCTG
GTTTCCATGA
GCCGGTGTCC
ACGCTCGGTG
ACGAAAAGGC
TGTCCGTGTC
CCCGTATACA
GACTTGAGAG
GGAGTTTAAA
CGAATTCAAT
AGCTTGTTGC
ATGGGCGGCG
ATATAAAATG
CAAGGTGCTG
CTCAAAAAAT
CAGGCAAAGC
CTCGCGCAAA
AAAGAAAGCA
CATCGTAGTC
ATGCTCATGC
AGATAAAGGC
AGGTAAGCTC
CGGAACCACC
ACAGAAAAAG
ACACCATTTT
TCTCTCAAAC
ATGTCTGCGG
GTTTCTGCAT
AAACACAAAA
TAAAATAACA
AAAAAACATT
TAAACATTAG
AAGCCTGTCT
TACAACAGGA
AAAACAACCC
TTATAAGCAT
AAGACGGACT
ACGGCCATGC
CGGCGTGACC
GTAAAAAAAC
TGGTCACCGT
GATTAAAAAG
CACCACCGAC
AGCTCCTCGG
TCATGTCCGG
AGTCATAATG
TAAGACTCGG
TAAACACATC
AGGTTGATTC
ACATCGGTCA
GTGCTAAAAA
GCGACCGAAA
TAGCCCGGGG
GAATACATAC
CCGCAGGCGT
AGAGACAACA
TTACAGCCCC
CATAGGAGGT
ATAACAAAAT
TAATAGGAGA
GAAAAACACA
TAAACACCTG
AAAAACCCTC
CTGCCTAGGC
AAAATAGCAC
CCTCCCGCTC
CAGAACAACA
TACAGCGCTT
CCACAGCGGC
AGCCATAACA
GTCAGCCTTA
CCAGTAAAAA
AGAAAACCTA
TTAAAAAAAC
ACCACTCGAC
ACGGCACCAG
CTCAATCAGT
CACAGTGTAA
AAAAGGGCCA
AGTGCAGAGC
GAGTATATAT
AGGACTAAAA
AATGACGTAA
CGGTTAAAGT
CCACAAAAAA
CACCCAGAAA
ACCGCACGCG
AACCTACGCC
CAGAAACGAA
AGCCAAAAAA
CCCACAACTT
CCTCAAATCG
TCACTTCCGT
TTTCCCACGT
TACGTCACTT
CCCATTTTAA
GAAAACTACA
ATTCCCAACA
CATACAAGTT
ACTCCGCCCT
AAAACCTACG
TCACCCGCCC
CGTTCCCACG
CCCCGCGCCA
CGTCACAAAC
TCCACCCCCT
CATTATCATA
TTGGCTTCAA
TCCAAAATAA
GGTATATTAT
TGATGATGTT
AATTAACATG
CATGGATCCA
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGGA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AAACTTGGTC
TGACAGTTAC
CAATGCTTAA
TCAGTGAGGC
ACCTATCTCA
GCGATCTGTC
TATTTCGTTC
ATCCATAGTT
GCCTGACTCC
CCGTCGTGTA
GATAACTACG
ATACGGGAGG
GCTTACCATC
TGGCCCCAGT
GCTGCAATGA
TACCGCGAGA
CCCACGCTCA
CCGGCTCCAG
ATTTATCAGC
AATAAACCAG
CCAGCCGGAA
GGGCCGAGCG
CAGAAGTGGT
CCTGCAACTT
TATCCGCCTC
CATCCAGTCT
ATTAATTGTT
GCCGGGAAGC
TAGAGTAAGT
AGTTCGCCAG
TTAATAGTTT
GCGCAACGTT
GTTGCCATTG
CTGCAGCCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTCACG
TAGAAAGCCA
GTCCGCAGAA
ACGGTGCTGA
CCCCGGATGA
ATGTCAGCTA
CTGGGCTATC
TGGACAAGGG
AAAACGCAAG
CGCAAAGAGA
AAGCAGGTAG
CTTGCAGTGG
GCTTACATGG
CGATAGCTAG
ACTGGGCGGT
TTTATGGACA
GCAAGCGAAC
CGGAATTGCC
AGCTGGGGCG
CCCTCTGGTA
AGGTTGGGAA
GCCCTGCAAA
GTAAACTGGA
TGGCTTTCTT
GCCGCCAAGG
ATCTGATGGC
GCAGGGGATC
AAGCTCTGAT
CAAGAGACAG
GATGAGGATC
GTTTCGCATG
ATTGAACAAG
ATGGATTGCA
CGCAGGTTCT
CCGGCCGCTT
GGGTGGAGAG
GCTATTCGGC
TATGACTGGG
CACAACAGAC
AATCGGCTGC
TCTGATGCCG
CCGTGTTCCG
GCTGTCAGCG
CAGGGGCGCC
CGGTTCTTTT
TGTCAAGACC
GACCTGTCCG
GTGCCCTGAA
TGAACTGCAA
GACGAGGCAG
CGCGGCTATC
GTGGCTGGCC
ACGACGGGCG
TTCCTTGCGC
AGCTGTGCTC
GACGTTGTCA
CTGAAGCGGG
AAGGGACTGG
CTGCTATTGG
GCGAAGTGCC
GGGGCAGGAT
CTCCTGTCAT
CTCACCTTGC
TCCTGCCGAG
AAAGTATCCA
TCATGGCTGA
TGCAATGCGG
CGGCTGCATA
CGCTTGATCC
GGCTACCTGC
CCATTCGACC
ACCAAGCGAA
ACATCGCATC
GAGCGAGCAC
GTACTCGGAT
GGAAGCCGGT
CTTGTCGATC
AGGATGATCT
GGACGAAGAG
CATCAGGGGC
TCGCGCCAGC
CGAACTGTTC
GCCAGGCTCA
AGGCGAGCAT
GCCCGACGGC
GAGGATCTCG
TCGTGACCCA
TGGCGATGCC
TGCTTGCCGA
ATATCATGGT
GGAAAATGGC
CGCTTTTCTG
GATTCATCGA
CTGTGGCCGG
CTGGGTGTGG
CGGACCGCTA
TCAGGACATA
GCGTTGGCTA
CCCGTGATAT
TGCTGAAGAG
CTTGGCGGCG
AATGGGCTGA
CCGCTTCCTC
GTGCTTTACG
GTATCGCCGC
TCCCGATTCG
CAGCGCATCG
CCTTCTATCG
CCTTCTTGAC
GAGTTCTTCT
GAATTTTGTT
AAAATTTTTG
TTAAATCAGC
TCATTTTTTA
ACCAATAGGC
CGAAATCGGC
ACCATCCCTT
ATAAATCAAA
AGAATAGACC
GAGATAGGGT
TGAGTGTTGT
TCCAGTTTGG
AACAAGAGTC
CACTATTAAA
GAACGTGGAC
TCCAACGTCA
AAGGGCGAAA
AACCGTCTAT
CAGGGCGATG
GCCCACTACG
TGAACCATCA
CCCTAATCAA
GTTTTTTGTG
GTCGAGGTGC
CGTAAAGCAC
TAAATCGGAA
CCCTAAAGGG
AGCCCCCGAT
TTAGAGCTTG
ACGGGGAAAG
CCGGCGAACG
TGGCGAGAAA
GGAAGGGAAG
AAAGCGAAAG
GAGCGGGCGC
TAGGGCGCTG
GCAAGTGTAG
CGGTCACGCT
GCGCGTAACC
ACCACACCCG
CGCGCTTAAT
GCGCCGCTAC
AGGGCGCGTC
CATTCGCCAT
TCAGGATCGA
ATTAATTCTT
AATTAA