名称: | pLVX-EF1a-IRES-mCherry |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 红色 |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | WPRE-R: |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATGAGTAA
TTCATACAAA
AGGACTCGCC
CCTGCCTTGG
GGAATCCCAG
GGACCGTCGT
TAAACTCCCA
CTAACGTAGA
ACCCAGAGAT
CGCTGCGTTC
CCGCCCCCTC
ACCCGCCCGC
TCTCGTCATC
ACTGAGGTGG
AGAAGAGCAT
GCGTGAGGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACCGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGGTA
AGTGCCGTGT
GTGGTTCCCG
CGGGCCTGGC
CTCTTTACGG
GTTATGGCCC
TTGCGTGCCT
TGAATTACTT
CCACGCCCCT
GGCTGCAGTA
CGTGATTCTT
GATCCCGAGC
TTCGGGTTGG
AAGTGGGTGG
GAGAGTTCGA
GGCCTTGCGC
TTAAGGAGCC
CCTTCGCCTC
GTGCTTGAGT
TGAGGCCTGG
CTTGGGCGCT
GGGGCCGCCG
CGTGCGAATC
TGGTGGCACC
TTCGCGCCTG
TCTCGCTGCT
TTCGATAAGT
CTCTAGCCAT
TTAAAATTTT
TGATGACCTG
CTGCGACGCT
TTTTTTCTGG
CAAGATAGTC
TTGTAAATGC
GGGCCAAGAT
CTGCACACTG
GTATTTCGGT
TTTTGGGGCC
GCGGGCGGCG
ACGGGGCCCG
TGCGTCCCAG
CGCACATGTT
CGGCGAGGCG
GGGCCTGCGA
GCGCGGCCAC
CGAGAATCGG
ACGGGGGTAG
TCTCAAGCTG
GCCGGCCTGC
TCTGGTGCCT
GGCCTCGCGC
CGCCGTGTAT
CGCCCCGCCC
TGGGCGGCAA
GGCTGGCCCG
GTCGGCACCA
GTTGCGTGAG
CGGAAAGATG
GCCGCTTCCC
GGCCCTGCTG
CAGGGAGCTC
AAAATGGAGG
ACGCGGCGCT
CGGGAGAGCG
GGCGGGTGAG
TCACCCACAC
AAAGGAAAAG
GGCCTTTCCG
TCCTCAGCCG
TCGCTTCATG
TGACTCCACG
GAGTACCGGG
CGCCGTCCAG
GCACCTCGAT
TAGTTCTCGA
GCTTTTGGAG
TACGTCGTCT
TTAGGTTGGG
GGGAGGGGTT
TTATGCGATG
GAGTTTCCCC
ACACTGAGTG
GGTGGAGACT
GAAGTTAGGC
CAGCTTGGCA
CTTGATGTAA
TTCTCCTTGG
AATTTGCCCT
TTTTGAGTTT
GGATCTTGGT
TCATTCTCAA
GCCTCAGACA
GTGGTTCAAA
GTTTTTTTCT
TCCATTTCAG
GTGTCGTGAG
GATCTATTTC
CGGTGAATTC
CTCGAGACTA
GTTCTAGAGC
GGCCGCGGAT
CCCGCCCCTC
TCCCTCCCCC
CCCCCTAACG
TTACTGGCCG
AAGCCGCTTG
GAATAAGGCC
GGTGTGCGTT
TGTCTATATG
TTATTTTCCA
CCATATTGCC
GTCTTTTGGC
AATGTGAGGG
CCCGGAAACC
TGGCCCTGTC
TTCTTGACGA
GCATTCCTAG
GGGTCTTTCC
CCTCTCGCCA
AAGGAATGCA
AGGTCTGTTG
AATGTCGTGA
AGGAAGCAGT
TCCTCTGGAA
GCTTCTTGAA
GACAAACAAC
GTCTGTAGCG
ACCCTTTGCA
GGCAGCGGAA
CCCCCCACCT
GGCGACAGGT
GCCTCTGCGG
CCAAAAGCCA
CGTGTATAAG
ATACACCTGC
AAAGGCGGCA
CAACCCCAGT
GCCACGTTGT
GAGTTGGATA
GTTGTGGAAA
GAGTCAAATG
GCTCACCTCA
AGCGTATTCA
ACAAGGGGCT
GAAGGATGCC
CAGAAGGTAC
CCCATTGTAT
GGGATCTGAT
CTGGGGCCTC
GGTGCACATG
CTTTACATGT
GTTTAGTCGA
GGTTAAAAAA
CGTCTAGGCC
CCCCGAACCA
CGGGGACGTG
GTTTTCCTTT
GAAAAACACG
ATGATAAGCT
TGCCACAACC
CACAAGGAGA
CGACCTTCCA
TGGTGAGCAA
GGGCGAGGAG
GATAACATGG
CCATCATCAA
GGAGTTCATG
CGCTTCAAGG
TGCACATGGA
GGGCTCCGTG
AACGGCCACG
AGTTCGAGAT
CGAGGGCGAG
GGCGAGGGCC
GCCCCTACGA
GGGCACCCAG
ACCGCCAAGC
TGAAGGTGAC
CAAGGGTGGC
CCCCTGCCCT
TCGCCTGGGA
CATCCTGTCC
CCTCAGTTCA
TGTACGGCTC
CAAGGCCTAC
GTGAAGCACC
CCGCCGACAT
CCCCGACTAC
TTGAAGCTGT
CCTTCCCCGA
GGGCTTCAAG
TGGGAGCGCG
TGATGAACTT
CGAGGACGGC
GGCGTGGTGA
CCGTGACCCA
GGACTCCTCC
CTGCAGGACG
GCGAGTTCAT
CTACAAGGTG
AAGCTGCGCG
GCACCAACTT
CCCCTCCGAC
GGCCCCGTAA
TGCAGAAGAA
GACCATGGGC
TGGGAGGCCT
CCTCCGAGCG
GATGTACCCC
GAGGACGGCG
CCCTGAAGGG
CGAGATCAAG
CAGAGGCTGA
AGCTGAAGGA
CGGCGGCCAC
TACGACGCTG
AGGTCAAGAC
CACCTACAAG
GCCAAGAAGC
CCGTGCAGCT
GCCCGGCGCC
TACAACGTCA
ACATCAAGTT
GGACATCACC
TCCCACAACG
AGGACTACAC
CATCGTGGAA
CAGTACGAAC
GCGCCGAGGG
CCGCCACTCC
ACCGGCGGCA
TGGACGAGCT
GTACAAGTGA
ACGCGTCTGG
AACAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAGC
TGACGTCCTT
TCCATGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCCTGGAATT
AATTCTGCAG
TCGAGACCTA
GAAAAACATG
GAGCAATCAC
AAGTAGCAAT
ACAGCAGCTA
CCAATGCTGA
TTGTGCCTGG
CTAGAAGCAC
AAGAGGAGGA
GGAGGTGGGT
TTTCCAGTCA
CACCTCAGGT
ACCTTTAAGA
CCAATGACTT
ACAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GAGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAGACAA
GATATCCTTG
ATCTGTGGAT
CTACCACACA
CAAGGCTACT
TCCCTGATTA
GCAGAACTAC
ACACCAGGGC
CAGGGGTCAG
ATATCCACTG
ACCTTTGGAT
GGTGCTACAA
GCTAGTACCA
GTTGAGCCAG
ATAAGGTAGA
AGAGGCCAAT
AAAGGAGAGA
ACACCAGCTT
GTTACACCCT
GTGAGCCTGC
ATGGGATGGA
TGACCCGGAG
AGAGAAGTGT
TAGAGTGGAG
GTTTGACAGC
CGCCTAGCAT
TTCATCACGT
GGCCCGAGAG
CTGCATCCGG
AGTACTTCAA
GAACTGCTGA
TATCGAGCTT
GCTACAAGGG
ACTTTCCGCT
GGGGACTTTC
CAGGGAGGCG
TGGCCTGGGC
GGGACTGGGG
AGTGGCGAGC
CCTCAGATCC
TGCATATAAG
CAGCTGCTTT
TTGCCTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTAGTAGT
TCATGTCATC
TTATTATTCA
GTATTTATAA
CTTGCAAAGA
AATGAATATC
AGAGAGTGAG
AGGCCTTGAC
ATTGCTAGCG
TTTACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCGAC
GGATCGGGAG
ATCAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTATCATG
TCTGGATCAA
CTGGATAACT
CAAGCTAACC
AAAATCATCC
CAAACTTCCC
ACCCCATACC
CTATTACCAC
TGCCAATTAC
CTGTGGTTTC
ATTTACTCTA
AACCTGTGAT
TCCTCTGAAT
TATTTTCATT
TTAAAGAAAT
TGTATTTGTT
AAATATGTAC
TACAAACTTA
GTAGT