名称: | pCW-Cas9 |
---|---|
别称: | Plasmid#50661 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑,四环素诱导 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | TRE |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | M13R:CAGGAAACAGCTATGACC |
反向引物: | hPGK-R(2023.01.30合成):agaatgtgcgagacccagg |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AATTCTCGAC
CTCGAGACAA
ATGGCAGTAT
TCATCCACAA
TTTTAAAAGA
AAAGGGGGGA
TTGGGGGGTA
CAGTGCAGGG
GAAAGAATAG
TAGACATAAT
AGCAACAGAC
ATACAAACTA
AAGAATTACA
AAAACAAATT
ACAAAAATTC
AAAATTTTCG
GGTTTATTAC
AGGGACAGCA
GAGATCCACT
TTGGCCGCGA
ATCGATATGT
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GTATGTCGAG
TTTACTCCCT
ATCAGTGATA
GAGAACGATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ACTCCCTATC
AGTGATAGAG
AACGTATGTC
GAGTTTATCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGTT
TACTCCCTAT
CAGTGATAGA
GAACGTATGT
CGAGGTAGGC
GTGTACGGTG
GGAGGCCTAT
ATAAGCAGAG
CTCGTTTAGT
GAACCGTCAG
ATCGCCTGGA
GAATTGGCTA
GCGCCACCAT
GGACTATAAG
GACCACGACG
GAGACTACAA
GGATCATGAT
ATTGATTACA
AAGACGATGA
CGATAAGATG
GCCCCAAAGA
AGAAGCGGAA
GGTCGGTATC
CACGGAGTCC
CAGCAGCCGA
CAAGAAGTAC
AGCATCGGCC
TGGACATCGG
CACCAACTCT
GTGGGCTGGG
CCGTGATCAC
CGACGAGTAC
AAGGTGCCCA
GCAAGAAATT
CAAGGTGCTG
GGCAACACCG
ACCGGCACAG
CATCAAGAAG
AACCTGATCG
GAGCCCTGCT
GTTCGACAGC
GGCGAAACAG
CCGAGGCCAC
CCGGCTGAAG
AGAACCGCCA
GAAGAAGATA
CACCAGACGG
AAGAACCGGA
TCTGCTATCT
GCAAGAGATC
TTCAGCAACG
AGATGGCCAA
GGTGGACGAC
AGCTTCTTCC
ACAGACTGGA
AGAGTCCTTC
CTGGTGGAAG
AGGATAAGAA
GCACGAGCGG
CACCCCATCT
TCGGCAACAT
CGTGGACGAG
GTGGCCTACC
ACGAGAAGTA
CCCCACCATC
TACCACCTGA
GAAAGAAACT
GGTGGACAGC
ACCGACAAGG
CCGACCTGCG
GCTGATCTAT
CTGGCCCTGG
CCCACATGAT
CAAGTTCCGG
GGCCACTTCC
TGATCGAGGG
CGACCTGAAC
CCCGACAACA
GCGACGTGGA
CAAGCTGTTC
ATCCAGCTGG
TGCAGACCTA
CAACCAGCTG
TTCGAGGAAA
ACCCCATCAA
CGCCAGCGGC
GTGGACGCCA
AGGCCATCCT
GTCTGCCAGA
CTGAGCAAGA
GCAGACGGCT
GGAAAATCTG
ATCGCCCAGC
TGCCCGGCGA
GAAGAAGAAT
GGCCTGTTCG
GAAACCTGAT
TGCCCTGAGC
CTGGGCCTGA
CCCCCAACTT
CAAGAGCAAC
TTCGACCTGG
CCGAGGATGC
CAAACTGCAG
CTGAGCAAGG
ACACCTACGA
CGACGACCTG
GACAACCTGC
TGGCCCAGAT
CGGCGACCAG
TACGCCGACC
TGTTTCTGGC
CGCCAAGAAC
CTGTCCGACG
CCATCCTGCT
GAGCGACATC
CTGAGAGTGA
ACACCGAGAT
CACCAAGGCC
CCCCTGAGCG
CCTCTATGAT
CAAGAGATAC
GACGAGCACC
ACCAGGACCT
GACCCTGCTG
AAAGCTCTCG
TGCGGCAGCA
GCTGCCTGAG
AAGTACAAAG
AGATTTTCTT
CGACCAGAGC
AAGAACGGCT
ACGCCGGCTA
CATTGACGGC
GGAGCCAGCC
AGGAAGAGTT
CTACAAGTTC
ATCAAGCCCA
TCCTGGAAAA
GATGGACGGC
ACCGAGGAAC
TGCTCGTGAA
GCTGAACAGA
GAGGACCTGC
TGCGGAAGCA
GCGGACCTTC
GACAACGGCA
GCATCCCCCA
CCAGATCCAC
CTGGGAGAGC
TGCACGCCAT
TCTGCGGCGG
CAGGAAGATT
TTTACCCATT
CCTGAAGGAC
AACCGGGAAA
AGATCGAGAA
GATCCTGACC
TTCCGCATCC
CCTACTACGT
GGGCCCTCTG
GCCAGGGGAA
ACAGCAGATT
CGCCTGGATG
ACCAGAAAGA
GCGAGGAAAC
CATCACCCCC
TGGAACTTCG
AGGAAGTGGT
GGACAAGGGC
GCTTCCGCCC
AGAGCTTCAT
CGAGCGGATG
ACCAACTTCG
ATAAGAACCT
GCCCAACGAG
AAGGTGCTGC
CCAAGCACAG
CCTGCTGTAC
GAGTACTTCA
CCGTGTATAA
CGAGCTGACC
AAAGTGAAAT
ACGTGACCGA
GGGAATGAGA
AAGCCCGCCT
TCCTGAGCGG
CGAGCAGAAA
AAGGCCATCG
TGGACCTGCT
GTTCAAGACC
AACCGGAAAG
TGACCGTGAA
GCAGCTGAAA
GAGGACTACT
TCAAGAAAAT
CGAGTGCTTC
GACTCCGTGG
AAATCTCCGG
CGTGGAAGAT
CGGTTCAACG
CCTCCCTGGG
CACATACCAC
GATCTGCTGA
AAATTATCAA
GGACAAGGAC
TTCCTGGACA
ATGAGGAAAA
CGAGGACATT
CTGGAAGATA
TCGTGCTGAC
CCTGACACTG
TTTGAGGACA
GAGAGATGAT
CGAGGAACGG
CTGAAAACCT
ATGCCCACCT
GTTCGACGAC
AAAGTGATGA
AGCAGCTGAA
GCGGCGGAGA
TACACCGGCT
GGGGCAGGCT
GAGCCGGAAG
CTGATCAACG
GCATCCGGGA
CAAGCAGTCC
GGCAAGACAA
TCCTGGATTT
CCTGAAGTCC
GACGGCTTCG
CCAACAGAAA
CTTCATGCAG
CTGATCCACG
ACGACAGCCT
GACCTTTAAA
GAGGACATCC
AGAAAGCCCA
GGTGTCCGGC
CAGGGCGATA
GCCTGCACGA
GCACATTGCC
AATCTGGCCG
GCAGCCCCGC
CATTAAGAAG
GGCATCCTGC
AGACAGTGAA
GGTGGTGGAC
GAGCTCGTGA
AAGTGATGGG
CCGGCACAAG
CCCGAGAACA
TCGTGATCGA
AATGGCCAGA
GAGAACCAGA
CCACCCAGAA
GGGACAGAAG
AACAGCCGCG
AGAGAATGAA
GCGGATCGAA
GAGGGCATCA
AAGAGCTGGG
CAGCCAGATC
CTGAAAGAAC
ACCCCGTGGA
AAACACCCAG
CTGCAGAACG
AGAAGCTGTA
CCTGTACTAC
CTGCAGAATG
GGCGGGATAT
GTACGTGGAC
CAGGAACTGG
ACATCAACCG
GCTGTCCGAC
TACGATGTGG
ACCATATCGT
GCCTCAGAGC
TTTCTGAAGG
ACGACTCCAT
CGACAACAAG
GTGCTGACCA
GAAGCGACAA
GAACCGGGGC
AAGAGCGACA
ACGTGCCCTC
CGAAGAGGTC
GTGAAGAAGA
TGAAGAACTA
CTGGCGGCAG
CTGCTGAACG
CCAAGCTGAT
TACCCAGAGA
AAGTTCGACA
ATCTGACCAA
GGCCGAGAGA
GGCGGCCTGA
GCGAACTGGA
TAAGGCCGGC
TTCATCAAGA
GACAGCTGGT
GGAAACCCGG
CAGATCACAA
AGCACGTGGC
ACAGATCCTG
GACTCCCGGA
TGAACACTAA
GTACGACGAG
AATGACAAGC
TGATCCGGGA
AGTGAAAGTG
ATCACCCTGA
AGTCCAAGCT
GGTGTCCGAT
TTCCGGAAGG
ATTTCCAGTT
TTACAAAGTG
CGCGAGATCA
ACAACTACCA
CCACGCCCAC
GACGCCTACC
TGAACGCCGT
CGTGGGAACC
GCCCTGATCA
AAAAGTACCC
TAAGCTGGAA
AGCGAGTTCG
TGTACGGCGA
CTACAAGGTG
TACGACGTGC
GGAAGATGAT
CGCCAAGAGC
GAGCAGGAAA
TCGGCAAGGC
TACCGCCAAG
TACTTCTTCT
ACAGCAACAT
CATGAACTTT
TTCAAGACCG
AGATTACCCT
GGCCAACGGC
GAGATCCGGA
AGCGGCCTCT
GATCGAGACA
AACGGCGAAA
CCGGGGAGAT
CGTGTGGGAT
AAGGGCCGGG
ATTTTGCCAC
CGTGCGGAAA
GTGCTGAGCA
TGCCCCAAGT
GAATATCGTG
AAAAAGACCG
AGGTGCAGAC
AGGCGGCTTC
AGCAAAGAGT
CTATCCTGCC
CAAGAGGAAC
AGCGATAAGC
TGATCGCCAG
AAAGAAGGAC
TGGGACCCTA
AGAAGTACGG
CGGCTTCGAC
AGCCCCACCG
TGGCCTATTC
TGTGCTGGTG
GTGGCCAAAG
TGGAAAAGGG
CAAGTCCAAG
AAACTGAAGA
GTGTGAAAGA
GCTGCTGGGG
ATCACCATCA
TGGAAAGAAG
CAGCTTCGAG
AAGAATCCCA
TCGACTTTCT
GGAAGCCAAG
GGCTACAAAG
AAGTGAAAAA
GGACCTGATC
ATCAAGCTGC
CTAAGTACTC
CCTGTTCGAG
CTGGAAAACG
GCCGGAAGAG
AATGCTGGCC
TCTGCCGGCG
AACTGCAGAA
GGGAAACGAA
CTGGCCCTGC
CCTCCAAATA
TGTGAACTTC
CTGTACCTGG
CCAGCCACTA
TGAGAAGCTG
AAGGGCTCCC
CCGAGGATAA
TGAGCAGAAA
CAGCTGTTTG
TGGAACAGCA
CAAGCACTAC
CTGGACGAGA
TCATCGAGCA
GATCAGCGAG
TTCTCCAAGA
GAGTGATCCT
GGCCGACGCT
AATCTGGACA
AAGTGCTGTC
CGCCTACAAC
AAGCACCGGG
ATAAGCCCAT
CAGAGAGCAG
GCCGAGAATA
TCATCCACCT
GTTTACCCTG
ACCAATCTGG
GAGCCCCTGC
CGCCTTCAAG
TACTTTGACA
CCACCATCGA
CCGGAAGAGG
TACACCAGCA
CCAAAGAGGT
GCTGGACGCC
ACCCTGATCC
ACCAGAGCAT
CACCGGCCTG
TACGAGACAC
GGATCGACCT
GTCTCAGCTG
GGAGGCGACA
AAAGGCCGGC
GGCCACGAAA
AAGGCCGGCC
AGGCAAAAAA
GAAAAAGTAA
GGATCCGGGG
TTGGGGTTGC
GCCTTTTCCA
AGGCAGCCCT
GGGTTTGCGC
AGGGACGCGG
CTGCTCTGGG
CGTGGTTCCG
GGAAACGCAG
CGGCGCCGAC
CCTGGGTCTC
GCACATTCTT
CACGTCCGTT
CGCAGCGTCA
CCCGGATCTT
CGCCGCTACC
CTTGTGGGCC
CCCCGGCGAC
GCTTCCTGCT
CCGCCCCTAA
GTCGGGAAGG
TTCCTTGCGG
TTCGCGGCGT
GCCGGACGTG
ACAAACGGAA
GCCGCACGTC
TCACTAGTAC
CCTCGCAGAC
GGACAGCGCC
AGGGAGCAAT
GGCAGCGCGC
CGACCGCGAT
GGGCTGTGGC
CAATAGCGGC
TGCTCAGCAG
GGCGCGCCGA
GAGCAGCGGC
CGGGAAGGGG
CGGTGCGGGA
GGCGGGGTGT
GGGGCGGTAG
TGTGGGCCCT
GTTCCTGCCC
GCGCGGTGTT
CCGCATTCTG
CAAGCCTCCG
GAGCGCACGT
CGGCAGTCGG
CTCCCTCGTT
GACCGAATCA
CCGACCTCTC
TCCCCAGCAA
TTCACCATGA
CCGAGTACAA
GCCCACGGTG
CGCCTCGCCA
CCCGCGACGA
CGTCCCCAGG
GCCGTACGCA
CCCTCGCCGC
CGCGTTCGCC
GACTACCCCG
CCACGCGCCA
CACCGTCGAT
CCGGACCGCC
ACATCGAGCG
GGTCACCGAG
CTGCAAGAAC
TCTTCCTCAC
GCGCGTCGGG
CTCGACATCG
GCAAGGTGTG
GGTCGCGGAC
GACGGCGCCG
CGGTGGCGGT
CTGGACCACG
CCGGAGAGCG
TCGAAGCGGG
GGCGGTGTTC
GCCGAGATCG
GCCCGCGCAT
GGCCGAGTTG
AGCGGTTCCC
GGCTGGCCGC
GCAGCAACAG
ATGGAAGGCC
TCCTGGCGCC
GCACCGGCCC
AAGGAGCCCG
CGTGGTTCCT
GGCCACCGTC
GGCGTCTCGC
CCGACCACCA
GGGCAAGGGT
CTGGGCAGCG
CCGTCGTGCT
CCCCGGAGTG
GAGGCGGCCG
AGCGCGCCGG
GGTGCCCGCC
TTCCTGGAGA
CCTCCGCGCC
CCGCAACCTC
CCCTTCTACG
AGCGGCTCGG
CTTCACCGTC
ACCGCCGACG
TCGAGGTGCC
CGAAGGACCG
CGCACCTGGT
GCATGACCCG
CAAGCCCGGT
GCCGAAGGTA
GAGGTTCTCT
CCTCACTTGT
GGTGATGTTG
AAGAAAACCC
TGGTCCAATG
TCTAGACTGG
ACAAGAGCAA
AGTCATAAAC
GGAGCTCTGG
AATTACTCAA
TGGTGTCGGT
ATCGAAGGCC
TGACGACAAG
GAAACTCGCT
CAAAAGCTGG
GAGTTGAGCA
GCCTACCCTG
TACTGGCACG
TGAAGAACAA
GCGGGCCCTG
CTCGATGCCC
TGCCAATCGA
GATGCTGGAC
AGGCATCATA
CCCACTTCTG
CCCCCTGGAA
GGCGAGTCAT
GGCAAGACTT
TCTGCGGAAC
AACGCCAAGT
CATACCGCTG
TGCTCTCCTC
TCACATCGCG
ACGGGGCTAA
AGTGCATCTC
GGCACCCGCC
CAACAGAGAA
ACAGTACGAA
ACCCTGGAAA
ATCAGCTCGC
GTTCCTGTGT
CAGCAAGGCT
TCTCCCTGGA
GAACGCACTG
TACGCTCTGT
CCGCCGTGGG
CCACTTTACA
CTGGGCTGCG
TATTGGAGGA
ACAGGAGCAT
CAAGTAGCAA
AAGAGGAAAG
AGAGACACCT
ACCACCGATT
CTATGCCCCC
ACTTCTGAGA
CAAGCAATTG
AGCTGTTCGA
CCGGCAGGGA
GCCGAACCTG
CCTTCCTTTT
CGGCCTGGAA
CTAATCATAT
GTGGCCTGGA
GAAACAGCTA
AAGTGCGAAA
GCGGCGGGCC
GACCGACGCC
CTTGACGATT
TTGACTTAGA
CATGCTCCCA
GCCGATGCCC
TTGACGACTT
TGACCTTGAT
ATGCTGCCTG
CTGACGCTCT
TGACGATTTT
GACCTTGACA
TGCTCCCCGG
GTAACTAAGT
AAGGATCGAT
CCAAGATATC
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAGCTGA
CGTCCTTTCC
ATGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCC
TGTTTCGCCT
CGGCGTCCGG
ACTAGAGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATCTGCTTTT
TGCTTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTAGTAGTT
CATGTCATCT
TATTATTCAG
TATTTATAAC
TTGCAAAGAA
ATGAATATCA
GAGAGTGAGA
GGAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGGCTCTAG
CTATCCCGCC
CCTAACTCCG
CCCATCCCGC
CCCTAACTCC
GCCCAGTTCC
GCCCATTCTC
CGCCCCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCGGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
TTCAAATATG
TATCCGCTCA
TGAGACAATA
ACCCTGATAA
ATGCTTCAAT
AATATTGAAA
AAGGAAGAGT
ATGAGTATTC
AACATTTCCG
TGTCGCCCTT
ATTCCCTTTT
TTGCGGCATT
TTGCCTTCCT
GTTTTTGCTC
ACCCAGAAAC
GCTGGTGAAA
GTAAAAGATG
CTGAAGATCA
GTTGGGTGCA
CGAGTGGGTT
ACATCGAACT
GGATCTCAAC
AGCGGTAAGA
TCCTTGAGAG
TTTTCGCCCC
GAAGAACGTT
TTCCAATGAT
GAGCACTTTT
AAAGTTCTGC
TATGTGGCGC
GGTATTATCC
CGTATTGACG
CCGGGCAAGA
GCAACTCGGT
CGCCGCATAC
ACTATTCTCA
GAATGACTTG
GTTGAGTACT
CACCAGTCAC
AGAAAAGCAT
CTTACGGATG
GCATGACAGT
AAGAGAATTA
TGCAGTGCTG
CCATAACCAT
GAGTGATAAC
ACTGCGGCCA
ACTTACTTCT
GACAACGATC
GGAGGACCGA
AGGAGCTAAC
CGCTTTTTTG
CACAACATGG
GGGATCATGT
AACTCGCCTT
GATCGTTGGG
AACCGGAGCT
GAATGAAGCC
ATACCAAACG
ACGAGCGTGA
CACCACGATG
CCTGTAGCAA
TGGCAACAAC
GTTGCGCAAA
CTATTAACTG
GCGAACTACT
TACTCTAGCT
TCCCGGCAAC
AATTAATAGA
CTGGATGGAG
GCGGATAAAG
TTGCAGGACC
ACTTCTGCGC
TCGGCCCTTC
CGGCTGGCTG
GTTTATTGCT
GATAAATCTG
GAGCCGGTGA
GCGTGGGTCT
CGCGGTATCA
TTGCAGCACT
GGGGCCAGAT
GGTAAGCCCT
CCCGTATCGT
AGTTATCTAC
ACGACGGGGA
GTCAGGCAAC
TATGGATGAA
CGAAATAGAC
AGATCGCTGA
GATAGGTGCC
TCACTGATTA
AGCATTGGTA
ACTGTCAGAC
CAAGTTTACT
CATATATACT
TTAGATTGAT
TTAAAACTTC
ATTTTTAATT
TAAAAGGATC
TAGGTGAAGA
TCCTTTTTGA
TAATCTCATG
ACCAAAATCC
CTTAACGTGA
GTTTTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTTC
TTCTAGTGTA
GCCGTAGTTA
GGCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCCAA
TACGCAAACC
GCCTCTCCCC
GCGCGTTGGC
CGATTCATTA
ATGCAGCTGG
CACGACAGGT
TTCCCGACTG
GAAAGCGGGC
AGTGAGCGCA
ACGCAATTAA
TGTGAGTTAG
CTCACTCATT
AGGCACCCCA
GGCTTTACAC
TTTATGCTTC
CGGCTCGTAT
GTTGTGTGGA
ATTGTGAGCG
GATAACAATT
TCACACAGGA
AACAGCTATG
ACCATGATTA
CGCCAAGCGC
GCAATTAACC
CTCACTAAAG
GGAACAAAAG
CTGGAGCTGC
AAGCTTAATG
TAGTCTTATG
CAATACTCTT
GTAGTCTTGC
AACATGGTAA
CGATGAGTTA
GCAACATGCC
TTACAAGGAG
AGAAAAAGCA
CCGTGCATGC
CGATTGGTGG
AAGTAAGGTG
GTACGATCGT
GCCTTATTAG
GAAGGCAACA
GACGGGTCTG
ACATGGATTG
GACGAACCAC
TGAATTGCCG
CATTGCAGAG
ATATTGTATT
TAAGTGCCTA
GCTCGATACA
TAAACGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTGG
CGCCCGAACA
GGGACTTGAA
AGCGAAAGGG
AAACCAGAGG
AGCTCTCTCG
ACGCAGGACT
CGGCTTGCTG
AAGCGCGCAC
GGCAAGAGGC
GAGGGGCGGC
GACTGGTGAG
TACGCCAAAA
ATTTTGACTA
GCGGAGGCTA
GAAGGAGAGA
GATGGGTGCG
AGAGCGTCAG
TATTAAGCGG
GGGAGAATTA
GATCGCGATG
GGAAAAAATT
CGGTTAAGGC
CAGGGGGAAA
GAAAAAATAT
AAATTAAAAC
ATATAGTATG
GGCAAGCAGG
GAGCTAGAAC
GATTCGCAGT
TAATCCTGGC
CTGTTAGAAA
CATCAGAAGG
CTGTAGACAA
ATACTGGGAC
AGCTACAACC
ATCCCTTCAG
ACAGGATCAG
AAGAACTTAG
ATCATTATAT
AATACAGTAG
CAACCCTCTA
TTGTGTGCAT
CAAAGGATAG
AGATAAAAGA
CACCAAGGAA
GCTTTAGACA
AGATAGAGGA
AGAGCAAAAC
AAAAGTAAGA
CCACCGCACA
GCAAGCGGCC
GCTGATCTTC
AGACCTGGAG
GAGGAGATAT
GAGGGACAAT
TGGAGAAGTG
AATTATATAA
ATATAAAGTA
GTAAAAATTG
AACCATTAGG
AGTAGCACCC
ACCAAGGCAA
AGAGAAGAGT
GGTGCAGAGA
GAAAAAAGAG
CAGTGGGAAT
AGGAGCTTTG
TTCCTTGGGT
TCTTGGGAGC
AGCAGGAAGC
ACTATGGGCG
CAGCGTCAAT
GACGCTGACG
GTACAGGCCA
GACAATTATT
GTCTGGTATA
GTGCAGCAGC
AGAACAATTT
GCTGAGGGCT
ATTGAGGCGC
AACAGCATCT
GTTGCAACTC
ACAGTCTGGG
GCATCAAGCA
GCTCCAGGCA
AGAATCCTGG
CTGTGGAAAG
ATACCTAAAG
GATCAACAGC
TCCTGGGGAT
TTGGGGTTGC
TCTGGAAAAC
TCATTTGCAC
CACTGCTGTG
CCTTGGAATG
CTAGTTGGAG
TAATAAATCT
CTGGAACAGA
TTTGGAATCA
CACGACCTGG
ATGGAGTGGG
ACAGAGAAAT
TAACAATTAC
ACAAGCTTAA
TACACTCCTT
AATTGAAGAA
TCGCAAAACC
AGCAAGAAAA
GAATGAACAA
GAATTATTGG
AATTAGATAA
ATGGGCAAGT
TTGTGGAATT
GGTTTAACAT
AACAAATTGG
CTGTGGTATA
TAAAATTATT
CATAATGATA
GTAGGAGGCT
TGGTAGGTTT
AAGAATAGTT
TTTGCTGTAC
TTTCTATAGT
GAATAGAGTT
AGGCAGGGAT
ATTCACCATT
ATCGTTTCAG
ACCCACCTCC
CAACCCCGAG
GGGAC