名称: | pCHO1.0 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达,抗体表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Kan |
筛选标记: | MTX,Puro |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | PGK-F:caatagcagctttgctccttc |
反向引物: | CMV-R:CCGCAATCTCCAAGGAACC |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GATTGGCTAT
TGGCCATTGC
ATACGTTGTA
TCCATATCAT
AATATGTACA
ATTCGCCCTT
CATTTAATTC
TACCGGGTAG
GGGAGGCGCT
TTTCCCAAGG
CAGTCTGGAG
CATGCGCTTT
AGCAGCCCCG
CTGGGCACTT
GGCGCTACAC
AAGTGGCCTC
TGGCCTCGCA
CACATTCCAC
ATCCACCGGT
AGGCGCCAAC
CGGCTCCGTT
CTTTGGTGGC
CCCTTCGCGC
CACCTTCTAC
TCCTCCCCTA
GTCAGGAAGT
TCCCCCCCGC
CCCGCAGCTC
GCGTCGTGCA
GGACGTGACA
AATGGAAGTA
GCACGTCTCA
CTAGTCTCGT
GCAGATGGAC
AGCACCGCTG
AGCAATGGAA
GCGGGTAGGC
CTTTGGGGCA
GCGGCCAATA
GCAGCTTTGC
TCCTTCGCTT
TCTGGGCTCA
GAGGCTGGGA
AGGGGTGGGT
CCGGGGGCGG
GCTCAGGGGC
GGGCTCAGGG
GCGGGGCGGG
CGCCCGAAGG
TCCTCCGGAG
GCCCGGCATT
CTGCACGCTT
CAAAAGCGCA
CGTCTGCCGC
GCTGTTCTCC
TCTTCCTCAT
CTCCGGGCCT
TTCTCGAGCT
CAAGCTTGGG
GGGGGGGACA
GCTCAGGGCT
GCGATTTCGC
GCCAAACTTG
ACGGCAATCC
TAGCGTGAAG
GCTGGTAGGA
TTTTATCCCC
GCTGCCATCA
TGGTTCGACC
ATTGAACTGC
ATCGTCGCCG
TGTCCCAAAA
TATGGGGATT
GGCAAGAACG
GAGACCTACC
CTGGCCTCCG
CTCAGGAACG
AGTTCAAGTA
CTTCCAAAGA
ATGACCACAA
CCTCTTCAGT
GGAAGGTAAA
CAGAATCTGG
TGATTATGGG
TAGGAAAACC
TGGTTCTCCA
TTCCTGAGAA
GAATCGACCT
TTAAAGGACA
GAATTAATAT
AGTTCTCAGT
AGAGAACTCA
AAGAACCACC
ACGAGGAGCT
CATTTTCTTG
CCAAAAGTTT
GGATGATGCC
TTAAGACTTA
TTGAACAACC
GGAATTGGCA
AGTAAAGTAG
ACATGGTTTG
GATAGTCGGA
GGCAGTTCTG
TTTACCAGGA
AGCCATGAAT
CAACCAGGCC
ACCTCAGACT
CTTTGTGACA
AGGATCATGC
AGGAATTTGA
AAGTGACACG
TTTTTCCCAG
AAATTGATTT
GGGGAAATAT
AAACTTCTCC
CAGAATACCC
AGGCGTCCTC
TCTGAGGTCC
AGGAGGAAAA
AGGCATCAAG
TATAAGTTTG
AAGTCTACGA
GAAGAAAGAC
TAACAGGAAG
ATGCTTTCAA
GTTCTCTGCT
CCCCTCCTAA
AGCTATGCAT
TTTTATAAGA
CCATGGGACT
TTTGCTGGCT
TTAGATCGTA
CAAGTAAAGC
GGCCGCGACT
CTAGATCATA
ATCAGCCATA
CCACATTTGT
AGAGGTTTTA
CTTGCTTTAA
AAAACCTCCC
ACACCTCCCC
CTGAACCTGA
AACATAAAAT
GAATGCAATT
GTTGTTGTTA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
AAGGCGTAAA
TTGTAAGCGT
TAATAAATGA
AGTTCCTATA
CTATTTGAAG
AATAGGAACT
TCGGAATAGG
AACTTCTCGG
CCGATGGCCG
CGAATTGCAG
CGGAGCTTCT
CTGCAGAGGT
GAATTTTTTT
ATTGGTCCCC
ACCCTAAAAG
ACCCAGCGTA
TATTTATTAT
TGTACTTATT
ACAGCTGACC
TGGTTGTGGC
TGCTTGTGGT
ACTCCAGGAT
TCTGCTCTTC
ATGGATGTGG
GGATGACAGT
GGGAGAGATC
CTTCCCTGCC
AACACCTGGC
TCCATCCCTG
CAGTTATGGG
GTTGAAAATT
CCCCTTTTTC
CCTTAAGCTC
TCCCCCTGGT
TCTTTAAATT
ACATGATGTT
AGAATTTTTC
AATAAGCAAC
CCATGCACTT
GAGAAGAAAT
TCAACCCCTC
TTTTATTAAG
TTCCGGGATA
CCTGTGAACA
GCCCCCCCTC
CTTTTTTTTT
TTTTTTTTTT
TTTGAGACAG
AGTCTTACTC
TCAGTCGCAC
AGGCTGGAGT
GCATGCAGTG
CCGTGATCTC
GGCTCACTGC
AACCTCTGCC
TCTCGGGTTC
AAGCGGTTCT
CCTGCCTCAG
CCTCCAAAGT
AGCTGGGATT
TCAGGCGTTC
ACTACCACGC
CGGGCTAATT
TTTTTATTTT
TAGTAAAGAC
GGGGTTTCTC
CATATTGGGT
AGGCTGGTCT
CGAACTCCTG
ACTTCAGGTG
ATTTGCCTGC
CTCGGCCTCC
CAAAGTGCTG
GGATTACAGG
CGTGACCCAC
CGCAAGCAGC
CTAACCCCTC
TTTAATTTTT
AAAATTTTTA
AAAATTTTTT
TGAGACGGAA
TCTCGCTCTG
TCGCCAGGCT
GGAGAGCAGT
GGCGCGATCT
CGGCTAACTG
CAACCTCCGC
CTCTCGCCTG
ACACCTCTTT
TAATGAAAAC
CTTTCCATTA
CATCTCTGAA
ATGCTCCCAC
TTTGCACGGA
AAAAACGGCT
GCGAAGCCCC
CAATAGGAAA
CGCAACACGT
AGCAGGTGCT
CAGTGCATTT
AAAAATTTTG
AAGAATTTTA
TTATTAATAA
TGCGAAACTG
ACTACCCCTC
TGCTAAAAAC
TCATGCCCCA
AATGGCGCAT
GCGCTTGACT
TGCAACTTAA
CCAAACGCGT
CACAGGTACA
GGTGTAGGTG
GTTAGGGACC
CCGGGCGCTC
CCATCTGCTC
CCTGCCGCGC
CCTCTGCCTT
TCAGAGCCTC
AATCCTTTCG
GAAGATACTT
TTCAGTTTTC
CACTGGGTTA
AATGGTGATA
GGAAGAGCAA
TCATCCGTTA
AATGAAACCC
AAGGTTGAGA
CAGCGCTTTG
TAAAGCAAAG
TGCCCGGAGC
ATTCGTGAGG
GGGAGGAGTG
TACAGGTTTA
TTTTAGAGTC
CAGGCTGGAA
TTTGCATTGC
TTTTTGTGGA
TCCACACCTC
CCCCTCTCCC
AGCCCAGGAA
AAGCACTGCA
GTGGCTCTCC
TGTTTCACAA
AGTGAGTTTA
AGGAAAGATG
TACCGCAGGG
GGTTCATTTG
CACTGTGAGA
ACATCATTCA
TGCAAGAAAT
GCTGAGTACC
TACTATGTGC
TAAACAGCGG
GTTGGGCTTT
TCATAAGGGA
TAGAGTTACA
CAATTGAATG
CCAATTCATG
AGATATGAAA
TACATTCAAC
TGTTTCTTAG
GGTCAGATTA
GGACACAAAT
GCTAAACAGT
CTTCCTGCGC
CTCCCCCTGG
AGAGGAAATG
AAATAGAAAA
TGAAATTGTG
CCAGTAAACC
GCTTAGCCGG
GTGCCTGGGA
CATGGCAAAC
CAGTCCTGAA
ATAAATAACT
GTTTTATTAA
TAGCAATCTT
AGCTAATTAA
AATAGATAGC
GTTTATTGAG
CGTTGGGTAT
CAGGCACGGT
CCTAATTCTT
TTAGATGTCT
TTAGTTCGTT
TCACTCTCCC
CCAAACAATA
GGGTGGTATT
GATCACCTCC
GAGCAGGTGA
AATTGAGGCA
CAGAGAAATC
CTAGTAGCTG
GTAGAAGAAC
ACGCAGTGTG
GTCAAGCTAG
CAAGGTGTTT
GGTCCACTGC
TATATCTACA
AAACCCCTAA
CAATGCCTGG
TGTATAGATG
CTCAGTATGC
ATTTGTGGGA
TCAGTGATTC
CGATGCCTGC
TTCTTATAAA
GTTTTTATTT
AGAAATAATT
ACAGGTAAGG
AGTTGCAAAA
ACAGTATAGT
GGGATCGAGT
GTCCTTTTTC
CCTCGGCTTC
TCCCAGGGGT
ATCGTCTTAC
GTAACAATGT
CCAAACAGGG
AAATTGACTT
GGGTATAATC
CACAGACTCT
ATTCACCTTG
TAGATTGGTT
TTAATAGAAG
TATATCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GGATACTGGA
CAACTTGTAA
GCTAATATCG
TTGCTATGGT
TCTCGTTCTC
AGCTAAAACG
GCGCTCTTTA
CTTTGTGCAC
CTGAACACTG
CACACCGAGG
GCGACCACCG
CCCCCGAGAT
GCCCAGCTTC
TATTCTAGAG
CGCCGCGCCG
GCGCCGAATG
GGTTAACGGG
CGGGGGGACA
CGCCTCCGTG
CGCTTGCGCG
GCGTCCCTTC
GCCCCGCCTT
CGCAGCGCAG
TCACATGACC
CGCCCAACCG
GCGTCCGCCT
ATAAAAAGCT
GAGTGTTGAC
GTCAGCGTTC
TCTTCCGCCG
TCGTCGCCGC
CATCCTCGGC
GCGACTCGCT
TCTTTCGGTT
CTACCTGGGA
GAATCCACCG
CCATCCGCCA
ACGCGTCAGG
TGAGTAACCC
TGCCTCGCTG
GTGCCCCGGA
GCGGCGGCGG
GGCCTCCGCG
GGGCCTACGC
TGCCTAGCCT
GGGACGCTGG
GGCCCCCCGT
GCCAGGAGGA
GACGTAGCGG
CGGCGGGGCC
GGAGGACCCG
GGGCTGGGGA
AGCGGCCGCC
GCCATGTCTG
TGCGCCGCGC
TGTTCACCGA
GCCCTTTCTC
CGTTTCCGAG
GGCGCCATAA
CCTTGCCCAG
ACCTGGTTGG
AGCTGGGTTG
TTAGCGGGGA
TGGGGGTGGG
GAATGATGAT
GTGGCAGGCG
TAGTAATGGC
GGGCACCGCG
GTGGAAGCGG
GGAGACAGGG
AGGCGCCTTA
TGTAACCCGC
GGGCCGCGAG
TTTGAGATCG
ATTTTCTGCC
GGGGGACTAG
GGGCGGCAGG
GAAATGGCAG
AACGAGCAAA
GCGACACCTG
AAAGGCTCCC
CTTTTCCTTC
CAAATACCTT
CTCCTGATGC
TGTTAATCGT
CGACCTTAGG
CACTTGTCCT
TCCTATGACT
CCCAGATGTA
CAAAGACTCT
TATTGAGACA
CGAGGTGTAG
GCTTGTGGCA
GTTAGGGCCG
TTCCGGCTCC
CCGTTATTTC
CTGGGGTGGG
TGGCCTTGTC
TGATCCCGTT
TGCCAAGGGG
TGACCTTGCA
TTTTATGATG
AAGCTTCTTG
CTCGGGGAAG
TTTGGGTGGC
CCGGTGACAA
CGTGGAGGGG
GCTTTAGGAG
AGGATTCATC
CCTTACGTGT
TTGGCCCCAA
ATGAGACTTT
AGTATTTGTA
CCTGGTATCA
AGGAAATCTG
TTGACACTCA
GTTTTATTCC
TGAGCACTTT
TATTTCTGGG
TTGTCAATCA
TGAATGACAC
CTATTAACGG
TGGTTCCTTG
GAGATTGCGG
GGTGGCTCTT
AAGGGGTGTT
AGGCTGGCAA
GATTCGGTGG
GCTTGGGGCA
CCCCAGCACC
AACCCCCTCC
TGGCCTTGGG
CATGCTGGTT
CGTTTGGACT
GAACGCTCCG
TGCCATGCTG
TGTTCCTGGA
AATCACGAGC
TGGTCTGAGC
CTCCTTGTCT
TGCCCAGGTG
AACTAGATCC
CCTACCTGCG
CCTCTCTTCC
CCAGACCTGC
GCGCTACTGC
GGCTCGGGCG
GTCGCTCGCC
TGGCTCTGCT
CCATTTGACT
GTCTGTGTGC
AGTCGCAGAA
CTTCGAAGAG
GGTTTTGCGC
TCCATCCGTG
GCGTTTCGCT
TTTGTTCGGT
TTTGTTGTTT
ATTTCATTTT
TTTTTTCCGG
AGAGAGGCGA
GGCGGTGGTC
CACACCCGCC
CGAGGAGGAA
CGGTTCCGGG
CCGCCTAGGA
CTGTATACTC
TATATTATAC
TCTATGTTAT
ACTCTGTAAT
CCTACTCAAT
AAACGTGTCA
CGCCTGTGAA
ACCGTACTAA
GTCTCCCGTG
TCTTCTTATC
ACCATCAGGT
GACATCCTCG
CCCAGGCTGT
CAATCATGCC
GGTATCGATT
CCAGTAGCAC
CGGCCCCACG
CTGACAACCC
ACTCTTGCAG
CGTTAGCAGC
GCCCCTCTTA
ACAAGCCGAC
CCCCACCAGC
GTCGCGGTTA
CTAACACTCC
TCTCCCCGGG
GCATCCGCTA
CTCCCGAGCT
CGAATTCGTT
TATCGGCCAC
CGAGGCCAAG
ATCTGGCCGC
CATATACTGA
GTCATTAGGG
ACTTTCCAAT
GGGTTTTGCC
CAGTACATAA
GGTCAATAGG
GGTGAATCAA
CAGGAAAGTC
CCATTGGAGC
CAAGTACACT
GAGTCAATAG
GGACTTTCCA
TTGGGTTTTG
CCCAGTACAA
AAGGTCAATA
GGGGGTGAGT
CAATGGGTTT
TTCCCATTAT
TGGCACGTAC
ATAAGGTCAA
TAGGGGTGAG
TCATTGGGTT
TTTCCAGCCA
ATTTATAAAA
CGCCATGTAC
TTTCCCACCA
TTGACGTCAA
TGGGCTATTG
AAACTAATGC
AACGTGACCT
TTAAACGGTA
CTTTCCCATA
GCTGATTAAT
GGGAAAGTAC
CGTTCTCGAG
CCAATACACG
TCAATGGGAA
GTGAAAGGGC
AGCCAAAACG
TAACACCGCC
CCGGTTTTCC
CCTGGAAATT
CCATATTGGC
ACGCATTCTA
TTGGCTGAGC
TGCGTTCTAC
GTGGGTATAA
GAGGCGCGAC
CAGCGTCGGT
AGGGACCGGT
TTGCCGCCAG
AACACAGGTA
AGTGCCGTGT
GTGGTTCCCG
CGGGCCTGGC
CTCTTTACGG
GTTATGGCCC
TTGCGTGCCT
TGAATTACTT
CCACCTGGCT
GCAGTACGTG
ATTCTTGATC
CCGAGCTTCG
GGTTGGAAGT
GGGTGGGAGA
GTTCGAGGCC
TTGCGCTTAA
GGAGCCCCTT
CGCCTCGTGC
TTGAGTTGAG
GCCTGGCCTG
GGCGCTGGGG
CCGCCGCGTG
CGAATCTGGT
GGCACCTTCG
CGCCTGTCTC
GCTGCTTTCG
ATAAGTCTCT
AGCCATTTAA
AATTTTTGAT
GACCTGCTGC
GACGCTTTTT
TTCTGGCAAG
ATAGTCTTGT
AAATGCGGGC
CAAGATCTGC
ACACTGGTAT
TTCGGTTTTT
GGGGCCGCGG
GCGGCGACGG
GGCCCGTGCG
TCCCAGCGCA
CATGTTCGGC
GAGGCGGGGC
CTGCGAGCGC
GGCCACCGAG
AATCGGACGG
GGGTAGTCTC
AAGATGGCCG
GCCTGCTCTG
GTGCCTGGCC
TCGCGCCGCC
GTGTATCGCC
CCGCCCTGGG
CGGCAAGGCT
GGCCCGGTCG
GCACCAGTTG
CGTGAGCGGA
AAGATGGCCG
CTTCCCGGCC
CTGCTGCAGG
GAGCTCAAAA
TGGAGGACGC
GGCGCTCGGG
AGAGCGGGCG
GGTGAGTCAC
CCACACAAAG
GAAAAGGGCC
TTTCCGTCCT
CAGCCGTCGC
TTCATGTGAC
TCCACGGAGT
ACCGGGCGCC
GTCCAGGCAC
CTCGATTAGT
TCTCGAGCTT
TTGGAGTACG
TCGTCTTTAG
GTTGGGGGGA
GGGGTTTTAT
GCGATGGAGT
TTCCCCACAC
TGAGTGGGTG
GAGACTGAAG
TTAGGCCAGC
TTGGCACTTG
ATGTAATTCT
CCTTGGAATT
TGCCCTTTTT
GAGTTTGGAT
CTTGGTTCAT
TCTCAAGCCT
CAGACAGTGG
TTCAAAGTTT
TTTTCTTCCA
TTTCAGGTGT
CGTGAGGAAT
TTCAGGTACT
AGCGCTACCG
GACTCAGATC
CCCTACCTGC
GCCTCTCTTC
CCCAGACCTG
CGCGCTACTG
CGGCTCGGGC
GGTCGCTCGC
CTGGCTCTGC
TCCATTTGAC
TGTCTGTGTG
CAGTCGCAGA
ACTTCGAAGA
GGGTTTTGCG
CTCCATCCGT
GGCGTTTCGC
TTTTGTTCGG
TTTTGTTGTT
TATTTCATTT
TTTTTTTCCG
GAGAGAGGCG
AGGCGGTGGT
CCACACCCGC
CCGAGGAGGA
AGGATCTCGA
GCTCAAGCTT
GATATCGGCG
TTAATTAAGC
TTCGATCCAG
ACATGATAAG
ATACATTGAT
GAGTTTGGAC
AAACCACAAC
TAGAATGCAG
TGAAAAAAAT
GCTTTATTTG
TGAAATTTGT
GATGCTATTG
CTTTATTTGT
AACCATTATA
AGCTGCAATA
AACAAGTTAA
CAACAACAAT
TGCATTCATT
TTATGTTTCA
GGTTCAGGGG
GAGGTGTGGG
AGGTTTTTTA
AAGCAAGTAA
AACCTCTACA
AATGTGGTAT
GGCTGATTAT
GATCCGGCTG
CCTCGCGCGT
TTCGGTGATG
ACGGTGAAAA
CCTCTGACAC
ATGCAGCTCC
CGGAGACGGT
CACAGCTTGT
CTGTAAGCGG
ATGCCGGGAG
CAGACAAGCC
CGTCAGGGCG
CGTCAGCGGG
TGTTGGCGGG
TGTCGGGGCG
CAGCCATGAC
CCAGTCACGT
AGCGATAGCG
GAGTGTATTC
GAGCTCGGAC
ATTGATTATT
GACTAGACTG
GCCACCGAGG
CCGTCGACTG
GGGCTCGAGA
TCCACTAGTT
CTAGAATGGT
GGGGTTGCGC
CTTTTCCAAG
GCAGCCCTGG
GTTTGCGCAG
GGACGCGGCT
GCTCTGGGCG
TGGTTCCGGG
AAACGCAGCG
GCGCCGACCC
TGGGTCTCGC
ACATTCTTCA
CGTCCGTTCG
CAGCGTCACC
CGGATCTTCG
CCGCTACCCT
TGTGGGCCCC
CCGGCGACGC
TTCCTGCTCC
GCCCCTAAGT
CGGGAAGGTT
CCTTGCGGTT
CGCGGCGTGC
CGGACGTGAC
AAACGGAAGC
CGCACGTCTC
ACTAGTACCC
TCGCAGACGG
ACAGCGCCAG
GGAGCAATGG
CAGCGCGCCG
ACCGCGATGG
GCTGTGGCCA
ATAGCGGCTG
CTCAGCAGGG
CGCGCCGAGA
GCAGCGGCCG
GGAAGGGGCG
GTGCGGGAGG
CGGGGTGTGG
GGCGGTAGTG
TGGGCCCTGT
TCCTGCCCGC
GCGGTGTTCC
GCATTCTGCA
AGCCTCCGGA
GCGCACGTCG
GCAGTCGGCT
CCCTCGTTCT
CGAGTTGAGA
CGACCTTCCA
TGACCGAGTA
CAAGCCCACG
GTGCGCCTCG
CCACCCGCGA
CGACGTCCCC
CGGGCCGTAC
GCACCCTCGC
CGCCGCGTTC
GCCGACTACC
CCGCCACGCG
CCACACCGTC
GACCCGGACC
GCCACATCGA
GCGGGTCACC
GAGCTGCAAG
AACTCTTCCT
CACGCGCGTC
GGGCTCGACA
TCGGCAAGGT
GTGGGTCGCG
GACGACGGCG
CCGCGGTGGC
GGTCTGGACC
ACGCCGGAGA
GCGTCGAAGC
GGGGGCGGTG
TTCGCCGAGA
TCGGCCCGCG
CATGGCCGAG
TTGAGCGGTT
CCCGGCTGGC
CGCGCAGCAA
CAGATGGAAG
GCCTCCTGGC
GCCGCACCGG
CCCAAGGAGC
CCGCGTGGTT
CCTGGCCACC
GTCGGCGTCT
CGCCCGACCA
CCAGGGCAAG
GGTCTGGGCA
GCGCCGTCGT
GCTCCCCGGA
GTGGAGGCGG
CCGAGCGCGC
CGGGGTGCCC
GCCTTCCTGG
AGACCTCCGC
GCCCCGCAAC
CTCCCCTTCT
ACGAGCGGCT
CGGCTTCACC
GTCACCGCCG
ACGTCGAGGT
GCCCGAAGGA
CCGCGCACCT
GGTGCATGAC
CCGCAAGCCC
GGTGCCTGAC
GCCCGCCCCA
CGACCCGCAG
CGCCCGACCG
AAAGGAGCGC
ACGACCCCAT
GGCTCCGACC
GAAGCCACCC
GGGGCGGCCC
CGCCGACCCC
GCACCCGCCC
CCGAGGCCCA
CCGACTCTAG
AGGATCATAA
TCAGCCATAC
CACATTTGTA
GAGGTTTTAC
TTGCTTTAAA
AAACCTCCCA
CACCTCCCCC
TGAACCTGAA
ACATAAAATG
AATGCAATTG
TTGTTGTTAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGG
ATCGGTACCG
GTCGACGAGC
GTCACAGCAC
AACCTGCAAA
AACGGAGCTG
GGCTGCAGCT
GGGGCTGGCA
TGGACTTTCA
TTTCAGAGAT
TCGGTTTTTA
AGAAGATGCA
TGCCTAACGT
GTTCTTTTTT
TTTTCCAATG
ATTTGTAATA
TACATTTTAT
GACTGGAAAC
TTTTTTGTAC
AACACTCCAA
TAAACATTTT
GATTTTAGGT
TCTGCCTCTG
AGTTTATTCC
TGAGGGGAAG
CTCGAGCCGG
GGCCTCTGCC
CTAATGAAGC
GGATGTCTAA
GAAAGATCCC
TCCACCCCCA
AGGAAAAAGG
TCACTGGCTA
GTGTAGCTAG
TGTAAACAGG
ACCCAGGCGA
TGCATGGGAC
CCTGCCCTTT
TTTTTCTAGT
GAGCCTCCGA
CGCTGTTGCA
CAAGCTGACT
CTTCGTCACG
TGATGCGACC
GGGCTCCGCC
CCGGCGGCAA
CACGCTGTAT
AGACGCGCCG
GGTGCCTCGT
GCGTGACGAA
GAGTCAGCTT
GTGCAACAGC
GTCGCAGGCT
CACATTACCC
TGTTATCCCT
AGTTGACCTC
GTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCGGGGG
GGGGGGGCGC
TGAGGTCTGC
CTCGTGAAGA
AGGTGTTGCT
GACTCATACC
AGGCCTGAAT
CGCCCCATCA
TCCAGCCAGA
AAGTGAGGGA
GCCACGGTTG
ATGAGAGCTT
TGTTGTAGGT
GGACCAGTTG
GTGATTTTGA
ACTTTTGCTT
TGCCACGGAA
CGGTCTGCGT
TGTCGGGAAG
ATGCGTGATC
TGATCCTTCA
ACTCAGCAAA
AGTTCGATTT
ATTCAACAAA
GCCGCCGTCC
CGTCAAGTCA
GCGTAATGCT
CTGCCAGTGT
TACAACCAAT
TAACCAATTC
TGATTAGAAA
AACTCATCGA
GCATCAAATG
AAACTGCAAT
TTATTCATAT
CAGGATTATC
AATACCATAT
TTTTGAAAAA
GCCGTTTCTG
TAATGAAGGA
GAAAACTCAC
CGAGGCAGTT
CCATAGGATG
GCAAGATCCT
GGTATCGGTC
TGCGATTCCG
ACTCGTCCAA
CATCAATACA
ACCTATTAAT
TTCCCCTCGT
CAAAAATAAG
GTTATCAAGT
GAGAAATCAC
CATGAGTGAC
GACTGAATCC
GGTGAGAATG
GCAAAAGCTT
ATGCATTTCT
TTCCAGACTT
GTTCAACAGG
CCAGCCATTA
CGCTCGTCAT
CAAAATCACT
CGCATCAACC
AAACCGTTAT
TCATTCGTGA
TTGCGCCTGA
GCGAGACGAA
ATACGCGATC
GCTGTTAAAA
GGACAATTAC
AAACAGGAAT
CGAATGCAAC
CGGCGCAGGA
ACACTGCCAG
CGCATCAACA
ATATTTTCAC
CTGAATCAGG
ATATTCTTCT
AATACCTGGA
ATGCTGTTTT
CCCGGGGATC
GCAGTGGTGA
GTAACCATGC
ATCATCAGGA
GTACGGATAA
AATGCTTGAT
GGTCGGAAGA
GGCATAAATT
CCGTCAGCCA
GTTTAGTCTG
ACCATCTCAT
CTGTAACATC
ATTGGCAACG
CTACCTTTGC
CATGTTTCAG
AAACAACTCT
GGCGCATCGG
GCTTCCCATA
CAATCGATAG
ATTGTCGCAC
CTGATTGCCC
GACATTATCG
CGAGCCCATT
TATACCCATA
TAAATCAGCA
TCCATGTTGG
AATTTAATCG
CGGCCTCGAG
CAAGACGTTT
CCCGTTGAAT
ATGGCTCATA
ACACCCCTTG
TATTACTGTT
TATGTAAGCA
GACAGTTTTA
TTGTTCATGA
TGATATATTT
TTATCTTGTG
CAATGTAACA
TCAGAGATTT
TGAGACACAA
CGTGGCTTTC
CCCCCCCCCC
CATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGACGTC
TAAGAAACCA
TTATTATCAT
GACATTAACC
TATAAAAATA
GGCGTATCAC
GAGGCCCTTT
CGTCTCGCGC
GTTTCGGTGA
TGACGGTGAA
AACCTCTGAC
ACATGCAGCT
CCCGGAGACG
GTCACAGCTT
GTCTGTAAGC
GGATGCCGGG
AGCAGACAAG
CCCGTCAGGG
CGCGTCAGCG
GGTGTTGGCG
GGTGTCGGGG
CTGGCTTAAC
TATGCGGCAT
CAGAGCAGAT
TGTACTGAGA
GTGCACCA