名称: | pTALETF_v2 (NN) |
---|---|
别称: | Plasmid#32185 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | TALEN,GateWay |
片段物种: | |
原核抗性: | Amp,Chl |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DB3.1 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CTCATGACCA
AAATCCCTTA
ACGTGAGTTA
CGCGCGCGTC
GTTCCACTGA
GCGTCAGACC
CCGTAGAAAA
GATCAAAGGA
TCTTCTTGAG
ATCCTTTTTT
TCTGCGCGTA
ATCTGCTGCT
TGCAAACAAA
AAAACCACCG
CTACCAGCGG
TGGTTTGTTT
GCCGGATCAA
GAGCTACCAA
CTCTTTTTCC
GAAGGTAACT
GGCTTCAGCA
GAGCGCAGAT
ACCAAATACT
GTTCTTCTAG
TGTAGCCGTA
GTTAGCCCAC
CACTTCAAGA
ACTCTGTAGC
ACCGCCTACA
TACCTCGCTC
TGCTAATCCT
GTTACCAGTG
GCTGCTGCCA
GTGGCGATAA
GTCGTGTCTT
ACCGGGTTGG
ACTCAAGACG
ATAGTTACCG
GATAAGGCGC
AGCGGTCGGG
CTGAACGGGG
GGTTCGTGCA
CACAGCCCAG
CTTGGAGCGA
ACGACCTACA
CCGAACTGAG
ATACCTACAG
CGTGAGCTAT
GAGAAAGCGC
CACGCTTCCC
GAAGGGAGAA
AGGCGGACAG
GTATCCGGTA
AGCGGCAGGG
TCGGAACAGG
AGAGCGCACG
AGGGAGCTTC
CAGGGGGAAA
CGCCTGGTAT
CTTTATAGTC
CTGTCGGGTT
TCGCCACCTC
TGACTTGAGC
GTCGATTTTT
GTGATGCTCG
TCAGGGGGGC
GGAGCCTATG
GAAAAACGCC
AGCAACGCGG
CCTTTTTACG
GTTCCTGGCC
TTTTGCTGGC
CTTTTGCTCA
CATGTTCTTT
CCTGCGTTAT
CCCCTGATTC
TGTGGATAAC
CGTATTACCG
CCTTTGAGTG
AGCTGATACC
GCTCGCCGCA
GCCGAACGAC
CGAGCGCAGC
GAGTCAGTGA
GCGAGGAAGC
GGAAGGCGAG
AGTAGGGAAC
TGCCAGGCAT
CAAACTAAGC
AGAAGGCCCC
TGACGGATGG
CCTTTTTGCG
TTTCTACAAA
CTCTTTCTGT
GTTGTAAAAC
GACGGCCAGT
CTTAAGCTCG
GGCCCCCTGG
GCGGTTCTGA
TAACGAGTAA
TCGTTAATCC
GCAAATAACG
TAAAAACCCG
CTTCGGCGGG
TTTTTTTATG
GGGGGAGTTT
AGGGAAAGAG
CATTTGTCAG
AATATTTAAG
GGCGCCTGTC
ACTTTGCTTG
ATATATGAGA
ATTATTTAAC
CTTATAAATG
AGAAAAAAGC
AACGCACTTT
AAATAAGATA
CGTTGCTTTT
TCGATTGATG
AACACCTATA
ATTAAACTAT
TCATCTATTA
TTTATGATTT
TTTGTATATA
CAATATTTCT
AGTTTGTTAA
AGAGAATTAA
GAAAATAAAT
CTCGAAAATA
ATAAAGGGAA
AATCAGTTTT
TGATATCAAA
ATTATACATG
TCAACGATAA
TACAAAATAT
AATACAAACT
ATAAGATGTT
ATCAGTATTT
ATTATCATTT
AGAATAAATT
TTGTGTCGCC
CTTAATTGTG
AGCGGATAAC
AATTACGAGC
TTCATGCACA
GTGGCGTTGA
CATTGATTAT
TGACTAGTTA
TTAATAGTAA
TCAATTACGG
GGTCATTAGT
TCATAGCCCA
TATATGGAGT
TCCGCGTTAC
ATAACTTACG
GTAAATGGCC
CGCCTGGCTG
ACCGCCCAAC
GACCCCCGCC
CATTGACGTC
AATAATGACG
TATGTTCCCA
TAGTAACGCC
AATAGGGACT
TTCCATTGAC
GTCAATGGGT
GGAGTATTTA
CGGTAAACTG
CCCACTTGGC
AGTACATCAA
GTGTATCATA
TGCCAAGTAC
GCCCCCTATT
GACGTCAATG
ACGGTAAATG
GCCCGCCTGG
CATTATGCCC
AGTACATGAC
CTTATGGGAC
TTTCCTACTT
GGCAGTACAT
CTACGTATTA
GTCATCGCTA
TTACCATGGT
GATGCGGTTT
TGGCAGTACA
TCAATGGGCG
TGGATAGCGG
TTTGACTCAC
GGGGATTTCC
AAGTCTCCAC
CCCATTGACG
TCAATGGGAG
TTTGTTTTGG
CACCAAAATC
AACGGGACTT
TCCAAAATGT
CGTAACAACT
CCGCCCCATT
GACGCAAATG
GGCGGTAGGC
GTGTACGGTG
GGAGGTCTAT
ATAAGCAGAG
CTCTCTGGCT
AACTAGAGAA
CCCACTGCTT
ACTGGCTTAT
CGAAATTAAT
ACGACTCACT
ATAGGGGCGT
ACGGCCACCA
TGTCGCGGAC
CCGGCTCCCT
TCCCCACCCG
CACCCAGCCC
AGCGTTTTCG
GCCGACTCGT
TCTCAGACCT
GCTTAGGCAG
TTCGACCCCT
CACTGTTTAA
CACATCGTTG
TTCGACTCCC
TTCCTCCGTT
TGGGGCGCAC
CATACGGAGG
CGGCCACCGG
GGAGTGGGAT
GAGGTGCAGT
CGGGATTGAG
AGCTGCGGAT
GCACCACCCC
CAACCATGCG
GGTGGCCGTC
ACCGCTGCCC
GACCGCCGAG
GGCGAAGCCC
GCACCAAGGC
GGAGGGCAGC
GCAACCGTCC
GACGCAAGCC
CCGCAGCGCA
AGTAGATTTG
AGAACTTTGG
GATATTCACA
GCAGCAGCAG
GAAAAGATCA
AGCCCAAAGT
GAGGTCGACA
GTCGCGCAGC
ATCACGAAGC
GCTGGTGGGT
CATGGGTTTA
CACATGCCCA
CATCGTAGCC
TTGTCGCAGC
ACCCTGCAGC
CCTTGGCACG
GTCGCCGTCA
AGTACCAGGA
CATGATTGCG
GCGTTGCCGG
AAGCCACACA
TGAGGCGATC
GTCGGTGTGG
GGAAACAGTG
GAGCGGAGCC
CGAGCGCTTG
AGGCCCTGTT
GACGGTCGCG
GGAGAGCTGA
GAGGGCCTCC
CCTTCAGCTG
GACACGGGCC
AGTTGCTGAA
GATCGCGAAG
CGGGGAGGAG
TCACGGCGGT
CGAGGCGGTG
CACGCGTGGC
GCAATGCGCT
CACGGGAGCA
CCCCTCAACC
TGACAGAGAC
CGCGGCCGCA
TTAGGCACCC
CAGGCTTTAC
ACTTTATGCT
TCCGGCTCGT
ATAATGTGTG
GATTTTGAGT
TAGGATCCGT
CGAGATTTTC
AGGAGCTAAG
GAAGCTAAAA
TGGAGAAAAA
AATCACTGGA
TATACCACCG
TTGATATATC
CCAATGGCAT
CGTAAAGAAC
ATTTTGAGGC
ATTTCAGTCA
GTTGCTCAAT
GTACCTATAA
CCAGACCGTT
CAGCTGGATA
TTACGGCCTT
TTTAAAGACC
GTAAAGAAAA
ATAAGCACAA
GTTTTATCCG
GCCTTTATTC
ACATTCTTGC
CCGCCTGATG
AATGCTCATC
CGGAATTCCG
TATGGCAATG
AAAGACGGTG
AGCTGGTGAT
ATGGGATAGT
GTTCACCCTT
GTTACACCGT
TTTCCATGAG
CAAACTGAAA
CGTTTTCATC
GCTCTGGAGT
GAATACCACG
ACGATTTCCG
GCAGTTTCTA
CACATATATT
CGCAAGATGT
GGCGTGTTAC
GGTGAAAACC
TGGCCTATTT
CCCTAAAGGG
TTTATTGAGA
ATATGTTTTT
CGTCTCAGCC
AATCCCTGGG
TGAGTTTCAC
CAGTTTTGAT
TTAAACGTGG
CCAATATGGA
CAACTTCTTC
GCCCCCGTTT
TCACCATGGG
CAAATATTAT
ACGCAAGGCG
ACAAGGTGCT
GATGCCGCTG
GCGATTCAGG
TTCATCATGC
CGTTTGTGAT
GGCTTCCATG
TCGGCAGAAT
GCTTAATGAA
TTACAACAGT
ACTGCGATGA
GTGGCAGGGC
GGGGCGTAAA
GATCTGGATC
CGGCTTACTA
AAAGCCAGAT
AACAGTATGC
GTATTTGCGC
GCTGATTTTT
GCGGTATAAG
AATATATACT
GATATGTATA
CCCGAAGTAT
GTCAAAAAGA
GGTATGCTAT
GAAGCAGCGT
ATTACAGTGA
CAGTTGACAG
CGACAGCTAT
CAGTTGCTCA
AGGCATATAT
GATGTCAATA
TCTCCGGTCT
GGTAAGCACA
ACCATGCAGA
ATGAAGCCCG
TCGTCTGCGT
GCCGAACGCT
GGAAAGCGGA
AAATCAGGAA
GGGATGGCTG
AGGTCGCCCG
GTTTATTGAA
ATGAACGGCT
CTTTTGCTGA
CGAGAACAGG
GGCTGGTGAA
ATGCAGTTTA
AGGTTTACAC
CTATAAAAGA
GAGAGCCGTT
ATCGTCTGTT
TGTGGATGTA
CAGAGTGATA
TTATTGACAC
GCCCGGGCGA
CGGATGGTGA
TCCCCCTGGC
CAGTGCACGT
CTGCTGTCAG
ATAAAGTCTC
CCGTGAACTT
TACCCGGTGG
TGCATATCGG
GGATGAAAGC
TGGCGCATGA
TGACCACCGA
TATGGCCAGT
GTGCCGGTCT
CCGTTATCGG
GGAAGAAGTG
GCTGATCTCA
GCCACCGCGA
AAATGACATC
AAAAACGCCA
TTAACCTGAT
GTTCTGGGGA
ATATAAATGT
CAGGCTCCCT
TATACACAGC
CAGTCTGCAG
GTCGACGGTC
TCGACTCACG
CCTGAGCAGG
TAGTGGCTAT
TGCATCCAAT
AACGGGGGCA
GACCCGCACT
GGAGTCAATC
GTGGCCCAGC
TTTCGAGGCC
GGACCCCGCG
CTGGCCGCAC
TCACTAATGA
TCATCTTGTA
GCGCTGGCCT
GCCTCGGCGG
ACGACCCGCC
TTGGATGCGG
TGAAGAAGGG
GCTCCCGCAC
GCGCCTGCAT
TGATTAAGCG
GACCAACAGA
AGGATTCCCG
AGAGGACATC
ACATCGAGTG
GCAGATCACG
CGCAAGTGGT
CCGCGTGCTC
GGATTCTTCC
AGTGTCACTC
CCACCCCGCA
CAAGCGTTCG
ATGACGCCAT
GACTCAATTT
GGTATGTCGA
GACACGGACT
GCTGCAGCTC
TTTCGTAGAG
TCGGTGTCAC
AGAACTCGAG
GCCCGCTCGG
GCACACTGCC
TCCCGCCTCC
CAGCGGTGGG
ACAGGATTCT
CCAAGCGAGC
GGTATGAAAC
GCGCGAAGCC
TTCACCTACG
TCAACTCAGA
CACCTGACCA
GGCGAGCCTT
CATGCGTTCG
CAGACTCGCT
GGAGAGGGAT
TTGGACGCGC
CCTCGCCCAT
GCATGAAGGG
GACCAAACTC
GCGCGTCAGC
TAGCCCCAAG
AAGAAGAGAA
AGGTGGAGGC
CAGCGGTTCC
GGACGGGCTG
ACGCATTGGA
CGATTTTGAT
CTGGATATGC
TGGGAAGTGA
CGCCCTCGAT
GATTTTGACC
TTGACATGCT
TGGTTCGGAT
GCCCTTGATG
ACTTTGACCT
CGACATGCTC
GGCAGTGACG
CCCTTGATGA
TTTCGACCTG
GACATGCTGA
TTAACTCTAG
AGGCAGTGGA
GAGGGCAGAG
GAAGTCTGCT
AACATGCGGT
GACGTCGAGG
AGAATCCTGG
CCCAGTGAGC
AAGGGCGAGG
AGCTGTTCAC
CGGGGTGGTG
CCCATCCTGG
TCGAGCTGGA
CGGCGACGTA
AACGGCCACA
AGTTCAGCGT
GTCCGGCGAG
GGCGAGGGCG
ATGCCACCTA
CGGCAAGCTG
ACCCTGAAGT
TCATCTGCAC
CACCGGCAAG
CTGCCCGTGC
CCTGGCCCAC
CCTCGTGACC
ACCCTGACCT
ACGGCGTGCA
GTGCTTCAGC
CGCTACCCCG
ACCACATGAA
GCAGCACGAC
TTCTTCAAGT
CCGCCATGCC
CGAAGGCTAC
GTCCAGGAGC
GCACCATCTT
CTTCAAGGAC
GACGGCAACT
ACAAGACCCG
CGCCGAGGTG
AAGTTCGAGG
GCGACACCCT
GGTGAACCGC
ATCGAGCTGA
AGGGCATCGA
CTTCAAGGAG
GACGGCAACA
TCCTGGGGCA
CAAGCTGGAG
TACAACTACA
ACAGCCACAA
CGTCTATATC
ATGGCCGACA
AGCAGAAGAA
CGGCATCAAG
GTGAACTTCA
AGATCCGCCA
CAACATCGAG
GACGGCAGCG
TGCAGCTCGC
CGACCACTAC
CAGCAGAACA
CCCCCATCGG
CGACGGCCCC
GTGCTGCTGC
CCGACAACCA
CTACCTGAGC
ACCCAGTCCG
CCCTGAGCAA
AGACCCCAAC
GAGAAGCGCG
ATCACATGGT
CCTGCTGGAG
TTCGTGACCG
CCGCCGGGAT
CACTCTCGGC
ATGGACGAGC
TGTACAAGTA
AGAATTCAAA
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCACAACAC
TCAACCCTAT
CTCGGTCTAT
TCTTTTGATT
TATAAGGGAT
TTTGCCGATT
TCGGCCTATT
GGTTAAAAAA
TGAGCTGATT
TAACAAAAAT
TTAACGCGAA
TTAATTCTGT
GGAATGTGTG
TCAGTTAGGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CATAGTCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCTGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGCTTTT
GCAAAAAGCT
CCCGGGAGCT
TGTATATCCA
TTTTCGGATC
TGATCAGCAC
GTGATGAAAA
AGCCTGAACT
CACCGCGACG
TCTGTCGAGA
AGTTTCTGAT
CGAAAAGTTC
GACAGCGTCT
CCGACCTGAT
GCAGCTCTCG
GAGGGCGAAG
AATCTCGTGC
TTTCAGCTTC
GATGTAGGAG
GGCGTGGATA
TGTCCTGCGG
GTAAATAGCT
GCGCCGATGG
TTTCTACAAA
GATCGTTATG
TTTATCGGCA
CTTTGCATCG
GCCGCGCTCC
CGATTCCGGA
AGTGCTTGAC
ATTGGGGAAT
TCAGCGAGAG
CCTGACCTAT
TGCATCTCCC
GCCGTGCACA
GGGTGTCACG
TTGCAAGACC
TGCCTGAAAC
CGAACTGCCC
GCTGTTCTGC
AGCCGGTCGC
GGAGGCCATG
GATGCGATCG
CTGCGGCCGA
TCTTAGCCAG
ACGAGCGGGT
TCGGCCCATT
CGGACCGCAA
GGAATCGGTC
AATACACTAC
ATGGCGTGAT
TTCATATGCG
CGATTGCTGA
TCCCCATGTG
TATCACTGGC
AAACTGTGAT
GGACGACACC
GTCAGTGCGT
CCGTCGCGCA
GGCTCTCGAT
GAGCTGATGC
TTTGGGCCGA
GGACTGCCCC
GAAGTCCGGC
ACCTCGTGCA
CGCGGATTTC
GGCTCCAACA
ATGTCCTGAC
GGACAATGGC
CGCATAACAG
CGGTCATTGA
CTGGAGCGAG
GCGATGTTCG
GGGATTCCCA
ATACGAGGTC
GCCAACATCT
TCTTCTGGAG
GCCGTGGTTG
GCTTGTATGG
AGCAGCAGAC
GCGCTACTTC
GAGCGGAGGC
ATCCGGAGCT
TGCAGGATCG
CCGCGGCTCC
GGGCGTATAT
GCTCCGCATT
GGTCTTGACC
AACTCTATCA
GAGCTTGGTT
GACGGCAATT
TCGATGATGC
AGCTTGGGCG
CAGGGTCGAT
GCGACGCAAT
CGTCCGATCC
GGAGCCGGGA
CTGTCGGGCG
TACACAAATC
GCCCGCAGAA
GCGCGGCCGT
CTGGACCGAT
GGCTGTGTAG
AAGTACTCGC
CGATAGTGGA
AACCGACGCC
CCAGCACTCG
TCCGAGGGCA
AAGGAATAGC
ACGTGCTACG
AGATTTCGAT
TCCACCGCCG
CCTTCTATGA
AAGGTTGGGC
TTCGGAATCG
TTTTCCGGGA
CGCCGGCTGG
ATGATCCTCC
AGCGCGGGGA
TCTCATGCTG
GAGTTCTTCG
CCCACCCCAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGT
ATACCGTCGA
CCTCTAGCTA
GAGCTTGGCG
TAATCATGGT
CATTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGCGCTG
CGATGATACC
GCGAGAACCA
CGCTCACCGG
CTCCGGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATCGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATATTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTCAGTGTTA
CAACCAATTA
ACCAATTCTG
AACATTATCG
CGAGCCCATT
TATACCTGAA
TATGGCTCAT
AACACCCCTT
G