名称: | pCP-Cre |
---|---|
别称: | |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | |
片段物种: | |
原核抗性: | Chl |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | |
拷贝数: | |
感受态: | DH5a |
温度: | 30度 |
背景载体: | |
正向引物: | pCP-F:TCCCAGCCCTGTGTATAAC |
反向引物: | AmpNR:GAATAAGGGCGACACGGAAATG |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GAGACACAAC
GTGGCTTTGT
TGAATAAATC
GAACTTTTGC
TGAGTTGAAG
GATCAGATCA
CGCATCTTCC
CGACAACGCA
GACCGTTCCG
TGGCAAAGCA
AAAGTTCAAA
ATCACCAACT
GGTCCACCTA
CAACAAAGCT
CTCATCAACC
GTGGCTCCCT
CACTTTCTGG
CTGGATGATG
GGGCGATTCA
GGCCTGGTAT
GAGTCAGCAA
CACCTTCTTC
ACGAGGCAGA
CCTCAGCGCC
ACAGGTGCGG
TTGCTGGCGC
TAACCGTTTT
TATCAGGCTC
TGGGAGGCAG
AATAAATGAT
CATATCGTCA
ATTATTACCT
CCACGGGGAG
AGCCTGAGCA
AACTGGCCTC
AGGCATTTGA
GAAGCACACG
GTCACACTGC
TTCCGGTAGT
CAATAAACCG
GTAAACCAGC
AATAGACATA
AGCGGCTATT
TAACGACCCT
GCCCTGAACC
GACGACCGGG
TCGAATTTGC
TTTCGAATTT
CTGCCATTCA
TCCGCTTATT
ATCACTTATT
CAGGCGTAGC
AACCAGGCGT
TTAAGGGCAC
CAATAACTGC
CTTAAAAAAA
TTACGCCCCG
CCCTGCCACT
CATCGCAGTA
CTGTTGTAAT
TCATTAAGCA
TTCTGCCGAC
ATGGAAGCCA
TCACAAACGG
CATGATGAAC
CTGAATCGCC
AGCGGCATCA
GCACCTTGTC
GCCTTGCGTA
TAATATTTGC
CCATGGTGAA
AACGGGGGCG
AAGAAGTTGT
CCATATTGGC
CACGTTTAAA
TCAAAACTGG
TGAAACTCAC
CCAGGGATTG
GCTGAGACGA
AAAACATATT
CTCAATAAAC
CCTTTAGGGA
AATAGGCCAG
GTTTTCACCG
TAACACGCCA
CATCTTGCGA
ATATATGTGT
AGAAACTGCC
GGAAATCGTC
GTGGTATTCA
CTCCAGAGCG
ATGAAAACGT
TTCAGTTTGC
TCATGGAAAA
CGGTGTAACA
AGGGTGAACA
CTATCCCATA
TCACCAGCTC
ACCGTCTTTC
ATTGCCATAC
GGAATTCCGG
ATGAGCATTC
ATCAGGCGGG
CAAGAATGTG
AATAAAGGCC
GGATAAAACT
TGTGCTTATT
TTTCTTTACG
GTCTTTAAAA
AGGCCGTAAT
ATCCAGCTGA
ACGGTCTGGT
TATAGGTACA
TTGAGCAACT
GACTGAAATG
CCTCAAAATG
TTCTTTACGA
TGCCATTGGG
ATATATCAAC
GGTGGTATAT
CCAGTGATTT
TTTTCTCCAT
TTTAGCTTCC
TTAGCTCCTG
AAAATCTCGA
TAACTCAAAA
AATACGCCCG
GTAGTGATCT
TATTTCATTA
TGGTGAAAGT
TGGAACCTCT
TACGTGCCGA
TCAACGTCTC
ATTTTCGCCA
AAAGTTGGCC
CAGGGCTTCC
CGGTATCAAC
AGGGACACCA
GGATTTATTT
ATTCTGCGAA
GTGATCTTCC
GTCACAGGTA
TTTATTCGGC
GCAAAGTGCG
TCGGGTGATG
CTGCCAACTT
ACTGATTTAG
TGTATGATGG
TGTTTTTGAG
GTGCTCCAGT
GGCTTCTGTT
TCTATCAGCT
GTCCCTCCTG
TTCAGCTACT
GACGGGGTGG
TGCGTAACGG
CAAAAGCACC
GCCGGACATC
AGCGCTTGTT
TCGGCGTGGG
TATGGTGGCA
GGCCCCGTGG
CCGGGGGACT
GTTGGGCGCC
TGTAGTGCCA
TTTACCCCCA
TTCACTGCCA
GAGCCGTGAG
CGCAGCGAAC
TGAATGTCAC
GAAAAAGACA
GCGACTCAGG
TGCCTGATGG
TCGGAGACAA
AAGGAATATT
CAGCGATTTG
CCCGAGCTTG
CGAGGGTGCT
ACTTAAGCCT
TTAGGGTTTT
AAGGTCTGTT
TTGTAGAGGA
GCAAACAGCG
TTTGCGACAT
CCTTTTGTAA
TACTGCGGAA
CTGACTAAAG
TAGTGAGTTA
TACACAGGGC
TGGGATCTAT
TCTTTTTATC
TTTTTTTATT
CTTTCTTTAT
TCTATAAATT
ATAACCACTT
GAATATAAAC
AAAAAAAACA
CACAAAGGTC
TAGCGGAATT
TACAGAGGGT
CTAGCAGAAT
TTACAAGTTT
TCCAGCAAAG
GTCTAGCAGA
ATTTACAGAT
ACCCACAACT
CAAAGGAAAA
GGACTAGTAA
TTATCATTGA
CTAGCCCATC
TCAATTGGTA
TAGTGATTAA
AATCACCTAG
ACCAATTGAG
ATGTATGTCT
GAATTAGTTG
TTTTCAAAGC
AAATGAACTA
GCGATTAGTC
GCTATGACTT
AACGGAGCAT
GAAACCAAGC
TAATTTTATG
CTGTGTGGCA
CTACTCAACC
CCACGATTGA
AAACCCTACA
AGGAAAGAAC
GGACGGTATC
GTTCACTTAT
AACCAATACG
TTCAGATGAT
GAACATCAGT
AGGGAAAATG
CTTATGGTGT
ATTAGCTAAA
GCAACCAGAG
AGCTGATGAC
GAGAACTGTG
GAAATCAGGA
ATCCTTTGGT
TAAAGGCTTT
GAGATTTTCC
AGTGGACAAA
CTATGCCAAG
TTCTCAAGCG
AAAAATTAGA
ATTAGTTTTT
AGTGAAGAGA
TATTGCCTTA
TCTTTTCCAG
TTAAAAAAAT
TCATAAAATA
TAATCTGGAA
CATGTTAAGT
CTTTTGAAAA
CAAATACTCT
ATGAGGATTT
ATGAGTGGTT
ATTAAAAGAA
CTAACACAAA
AGAAAACTCA
CAAGGCAAAT
ATAGAGATTA
GCCTTGATGA
ATTTAAGTTC
ATGTTAATGC
TTGAAAATAA
CTACCATGAG
TTTAAAAGGC
TTAACCAATG
GGTTTTGAAA
CCAATAAGTA
AAGATTTAAA
CACTTACAGC
AATATGAAAT
TGGTGGTTGA
TAAGCGAGGC
CGCCCGACTG
ATACGTTGAT
TTTCCAAGTT
GAACTAGATA
GACAAATGGA
TCTCGTAACC
GAACTTGAGA
ACAACCAGAT
AAAAATGAAT
GGTGACAAAA
TACCAACAAC
CATTACATCA
GATTCCTACC
TACATAACGG
ACTAAGAAAA
ACACTACACG
ATGCTTTAAC
TGCAAAAATT
CAGCTCACCA
GTTTTGAGGC
AAAATTTTTG
AGTGACATGC
AAAGTAAGTA
TGATCTCAAT
GGTTCGTTCT
CATGGCTCAC
GCAAAAACAA
CGAACCACAC
TAGAGAACAT
ACTGGCTAAA
TACGGAAGGA
TCTGAGGTTC
TTATGGCTCT
TGTATCTATC
AGTGAAGCAT
CAAGACTAAC
AAACAAAAGT
AGAACAACTG
TTCACCGTTA
CATATCAAAG
GGAAAACTGT
CCATATGCAC
AGATGAAAAC
GGTGTAAAAA
AGATAGATAC
ATCAGAGCTT
TTACGAGTTT
TTGGTGCATT
TAAAGCTGTT
CACCATGAAC
AGATCGACAA
TGTAACAGAT
GAACAGCATG
TAACACCTAA
TAGAACAGGT
GAAACCAGTA
AAACAAAGCA
ACTAGAACAT
GAAATTGAAC
ACCTGAGACA
ACTTGTTACA
GCTCAACAGT
CACACATAGA
CAGCCTGAAA
CAGGCGATGC
TGCTTATCGA
ATCAAAGCTG
CCGACAACAC
GGGAGCCAGT
GACGCCTCCC
GTGGGGAAAA
AATCATGGCA
ATTCTGGAAG
AAATAGCGCC
TGTTTCGTTT
CAGGCAGGTT
ATCAGGGAGT
GTCAGCGTCC
TGCGGTTCTC
CGGGGCGTTC
GGGTCATGCA
GCCCGTAATG
GTGATTTACC
AGCGTCTGCC
AGGCATCAAT
TCTAGGCCTG
TCTGCGCGGT
CGTAGTACGG
CTGGAGGCGT
TTTCCGGTCT
GTAGCTCCAT
GTTCGGAATG
ACAAAATTCA
GCTCAAGCCG
TCCCTTGTCC
TGGTGCTCCA
CCCACAGGAT
GCTGTACTGA
TTTTTTTCGA
GACCGGGCAT
CAGTACACGC
TCAAAGCTCG
CCATCACTTT
TTCACGTCCT
CCCGGCGGCA
GCTCCTTCTC
CGCGAACGAC
AGAACACCGG
ACGTGTATTT
CTTCGCAAAT
GGCGTGGCAT
CGATGAGTTC
CCGGACTTCT
TCCGGATTAC
CCTGAAGCAC
CGTTGCGCCT
TCGCGGTTAC
GCTCCCTCCC
CAGCAGGTAA
TCAACCGGAC
CACTGCCACC
ACCTTTTCCC
CTGGCATGAA
ATTTAACTAT
CATCCCGCGC
CCCCTGTTCC
CTGACAGCCA
GACGCAGCCG
GCGCAGCTCA
TCCCCGATGG
CCATCAGTGC
GGCCACCACC
TGAACCCGGT
CACCGGAAGA
CCACTGCCCG
CTGTTCACCT
TACGGGCTGT
CTGATTCAGG
TTATTTCCGA
TGGCGGCCAG
CTGACGCAGT
AACGGCGGTG
CCAGTGTCGG
CAGTTTTCCG
GAACGGGCAA
CCGGCTCCCC
CAGGCAGACC
CGCCGCATCC
ATACCGCCAG
TTGTTTACCC
TCACAGCGTT
CAAGTAACCG
GGCATGTTCA
TCATCAGTAA
CCCGTATTGT
GAGCATCCTC
TCGCGTTTCA
TCGGTATCAT
TACCCCATGA
ACAGAAATCC
CCCTTACACG
GAGGCATCAG
TGACTAAACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AAACTTGGTC
TGACAGTTAC
CAATGCTTAA
TCAGTGAGGC
ACCTATCTCA
GCGATCTGTC
TATTTCGTTC
ATCCATAGTT
GCCTGACTCC
CCGTCGTGTA
GATAACTACG
ATACGGGAGG
GCTTACCATC
TGGCCCCAGT
GCTGCAATGA
TACCGCGAGA
CCCACGCTCA
CCGGCTCCAG
ATTTATCAGC
AATAAACCAG
CCAGCCGGAA
GGGCCGAGCG
CAGAAGTGGT
CCTGCAACTT
TATCCGCCTC
CATCCAGTCT
ATTAATTGTT
GCCGGGAAGC
TAGAGTAAGT
AGTTCGCCAG
TTAATAGTTT
GCGCAACGTT
GTTGCCATTG
CTGCAGGCAT
CGTGGTGTCA
CGCTCGTCGT
TTGGTATGGC
TTCATTCAGC
TCCGGTTCCC
AACGATCAAG
GCGAGTTACA
TGATCCCCCA
TGTTGTGCAA
AAAAGCGGTT
AGCTCCTTCG
GTCCTCCGAT
CGTTGTCAGA
AGTAAGTTGG
CCGCAGTGTT
ATCACTCATG
GTTATGGCAG
CACTGCATAA
TTCTCTTACT
GTCATGCCAT
CCGTAAGATG
CTTTTCTGTG
ACTGGTGAGT
ACTCAACCAA
GTCATTCTGA
GAATAGTGTA
TGCGGCGACC
GAGTTGCTCT
TGCCCGGCGT
CAACACGGGA
TAATACCGCG
CCACATAGCA
GAACTTTAAA
AGTGCTCATC
ATTGGAAAAC
GTTCTTCGGG
GCGAAAACTC
TCAAGGATCT
TACCGCTGTT
GAGATCCAGT
TCGATGTAAC
CCACTCGTGC
ACCCAACTGA
TCTTCAGCAT
CTTTTACTTT
CACCAGCGTT
TCTGGGTGAG
CAAAAACAGG
AAGGCAAAAT
GCCGCAAAAA
AGGGAATAAG
GGCGACACGG
AAATGTTGAA
TACTCATACT
CTTCCTTTTT
CAATATTATT
GAAGCATTTA
TCAGGGTTAT
TGTCTCATGA
GCGGATACAT
ATTTGAATGT
ATTTAGAAAA
ATAAACAAAT
AGGGGTTCCG
CGCACATTTC
CCCGAAAAGT
GCCACCTGAC
GTCTAAGAAA
CCATTATTAT
CATGACATTA
ACCTATAAAA
ATAGGCGTAT
CACGAGGCCC
TTTCGTCTTC
AAGAATTTTA
TAAACCGTGG
AGCGGGCAAT
ACTGAGCTGA
TGAGCAATTT
CCGTTGCACC
AGTGCCCTTC
TGATGAAGCG
TCAGCACGAC
GTTCCTGTCC
ACGGTACGCC
TGCGGCCAAA
TTTGATTCCT
TTCAGCTTTG
CTTCCTGTCG
GCCCTCATTC
GTGCGCTCTA
GGATCCCTAA
TCGCCATCTT
CCAGCAGGCG
CACCATTGCC
CCTGTTTCAC
TATCCAGGGT
ACGGATATAG
TTCATGACAA
TATTTACATT
GGTCCAGCCA
CCAGCTTGCA
TGATCTCCGG
TATTGAAACT
CCAGCGCGGG
CCATATCTCG
CGCGGCTCCG
ACACGGGCAC
TGTGTCCAGA
CCAGGCCAGG
TATCTCTGAC
CAGAGTCATC
CTTAGCGCCG
TAAATCAATC
GATGAGTTGC
TTCAAAAATC
CCTTCCAGGG
CGCGAGTTGA
TAGCTGGCTG
GTGGCAGATG
GCGCGGCAAC
ACCATTTTTT
CTGACCCGGC
AAAACAGGTA
GTTATTCGGA
TCATCAGCTA
CACCAGAGAC
GGAAATCCAT
CGCTCGACCA
GTTTAGTTAC
CCCCAGGCTA
AGTGCCTTCT
CTACACCTGC
GGTGCTAACC
AGCGTTTTCG
TTCTGCCAAT
ATGGATTAAC
ATTCTCCCAC
CGTCAGTACG
TGAGATATCT
TTAACCCTGA
TCCTGGCAAT
TTCGGCTATA
CGTAACAGGG
TGTTATAAGC
AATCCCCAGA
AATGCCAGAT
TACGTATATC
CTGGCAGCGA
TCGCTATTTT
CCATGAGTGA
ACGAACCTGG
TCGAAATCAG
TGCGTTCGAA
CGCTAGAGCC
TGTTTTGCAC
GTTCACCGGC
ATCAACGTTT
TCTTTTCGGA
TCCGCCGCAT
AACCAGTGAA
ACAGCATTGC
TGTCACTTGG
TCGTGGCAGC
CCGGACCGAC
GATGAAGCAT
GTTTAGCTGG
CCCAAATGTT
GCTGGATAGT
TTTTACTGCC
AGACCGCGCG
CCTGAAGATA
TAGAAGATAA
TCGCGAACAT
CTTCAGGTTC
TGCGGGAAAC
CATTTCCGGT
TATTCAACTT
GCACCATGCC
GCCCACGACC
GGCAAACGGA
CAGAAGCATT
TTCCAGGTAT
GCTCAGAAAA
CGCCTGGCGA
TCCCTGAACA
TGTCCATCAG
GTTCTTGCGA
ACCTCATCAC
TCGTTGCATC
GACCGGTAAT
GCAGGCAAAT
TTTGGTGTAC
GGTCAGTAAA
TTGGACATGG
TGAATTCCAG
CACACCCGCG
GCCTTGTTAT
CCGCTCACAA
TTCCACACAT
TATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAAACGATCT
CGATCCTCTA
CGCCGGACGC
ATCGTGGCCG
GCATCACCGG
CGCCACAGGT
GCGGTTGCTG
GCGCCTATAT
CGCCGACATC
ACCGATGGGG
AAGATCGGGC
TCGCCACTTC
GGGCTCATGA
GCGCTTGTTT
CGGCGTGGGT
ATGGTGGCAG
GCCCCGTGGC
CGGGGGACTG
TTGGGCGCCA
TCTCCTTGCA
TGCACCATTC
CTTGCGGCGG
CGGTGCTCAA
CGGCCTCAAC
CTACTACTGG
GCTGCTTCCT
AATGCAGGAG
TCGCATAAGG
GAGAGCGTCG
AGATCCCGGA
CACCATCGAA
TGGCGCAAAA
CCTTTCGCGG
TATGGCATGA
TAGCGCCCGG
AAGAGAGTCA
ATTCAGGGTG
GTGAATGTGA
AACCAGTAAC
GTTATACGAT
GTCGCAGAGT
ATGCCGGTGT
CTCTTATCAG
ACCGTTTCCC
GCGTGGTGAA
CCAGGCCAGC
CACGTTTCTG
CGAAAACGCG
GGAAAAAGTG
GAAGCGGCGA
TGGCGGAGCT
GAATTACATT
CCCAACCGCG
TGGCACAACA
ACTGGCGGGC
AAACAGTCGT
TGCTGATTGG
CGTTGCCACC
TCCAGTCTGG
CCCTGCACGC
GCCGTCGCAA
ATTGTCGCGG
CGATTAAATC
TCGCGCCGAT
CAACTGGGTG
CCAGCGTGGT
GGTGTCGATG
GTAGAACGAA
GCGGCGTCGA
AGCCTGTAAA
GCGGCGGTGC
ACAATCTTCT
CGCGCAACGC
GTCAGTGGGC
TGATCATTAA
CTATCCGCTG
GATGACCAGG
ATGCCATTGC
TGTGGAAGCT
GCCTGCACTA
ATGTTCCGGC
GTTATTTCTT
GATGTCTCTG
ACCAGACACC
CATCAACAGT
ATTATTTTCT
CCCATGAAGA
CGGTACGCGA
CTGGGCGTGG
AGCATCTGGT
CGCATTGGGT
CACCAGCAAA
TCGCGCTGTT
AGCGGGCCCA
TTAAGTTCTG
TCTCGGCGCG
TCTGCGTCTG
GCTGGCTGGC
ATAAATATCT
CACTCGCAAT
CAAATTCAGC
CGATAGCGGA
ACGGGAAGGC
GACTGGAGTG
CCATGTCCGG
TTTTCAACAA
ACCATGCAAA
TGCTGAATGA
GGGCATCGTT
CCCACTGCGA
TGCTGGTTGC
CAACGATCAG
ATGGCGCTGG
GCGCAATGCG
CGCCATTACC
GAGTCCGGGC
TGCGCGTTGG
TGCGGACATC
TCGGTAGTGG
GATACGACGA
TACCGAAGAC
AGCTCATGTT
ATATCCCGCC
GTTAACCACC
ATCAAACAGG
ATTTTCGCCT
GCTGGGGCAA
ACCAGCGTGG
ACCGCTTGCT
GCAACTCTCT
CAGGGCCAGG
CGGTGAAGGG
CAATCAGCTG
TTGCCCGTCT
CACTGGTGAA
AAGAAAAACC
ACCCTGGCGC
CCAATACGCA
AACCGCCTCT
CCCCGCGCGT
TGGCCGATTC
ATTAATGCAG
CTGGCACGAC
AGGTTTCCCG
ACTGGAAAGC
GGGCAGTGAC
CCGGGGATCG
CAGTGGTGAG
TAACCATGCA
TCATCAGGAG
TACGGATAAA
ATGCTTGATG
GTCGGAAGAG
GCATAAATTC
CGTCAGCCAG
TTTAGTCTGA
CCATCTCATC
TGTAACATCA
TTGGCAACGC
TACCTTTGCC
ATGTTTCAGA
AACAACTCTG
GCGCATCGGG
CTTCCCATAC
AATCGATAGA
TTGTCGCACC
TGATTGCCCG
ACATTATCGC
GAGCCCATTT
ATACCCATAT
AAATCAGCAT
CCATGTTGGA
ATTTAATCGC
GGCCTCGAGC
AAGACGTTTC
CCGTTGAATA
TGGCTCATAA
CACCCCTTGT
ATTACTGTTT
ATGTAAGCAG
ACAGTTTTAT
TGTTCATGAT
GATATATTTT
TATCTTGTGC
AATGTAACAT
CAGAGATTTT